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Target-dependent nickase activities of CRISPR-Cas nucleases Cpf1 and Cas9

Clustered regularly interspaced short palindromic repeat (CRISPR) machineries are prokaryotic immune systems that have been adapted as versatile gene editing and manipulation tools. We found that CRISPR-Cas nucleases Cpf1 (also known as Cas12a) and Cas9 exhibit differential guide RNA sequence requir...

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Detalles Bibliográficos
Autores principales:	Hua Fu, Becky Xu, Smith, Justin D., Fuchs, Ryan T., Mabuchi, Megumu, Curcuru, Jennifer, Robb, G. Brett, Fire, Andrew Z.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6512873/ https://www.ncbi.nlm.nih.gov/pubmed/30833733 http://dx.doi.org/10.1038/s41564-019-0382-0

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