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Assessment of TCF7L2 expression after bariatric surgery

OBJECTIVE: To assess the influence of bariatric surgery on transcription factor 7-like 2 (TCF7L2) expression and its association with body mass index (BMI) and Type 2 diabetes mellitus (T2DM). METHODS: Prospective study performed between 2016 and 2018, where 26 obese patients undergoing bariatric su...

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Autores principales: Macedo, Carlos Eduardo S., da Conti, Guilherme, Catena, Andriu S., Bruneska, Danyelly, Rosa, Malu, Noronha, Clarissa G., Santa Cruz, Fernando, Ferraz, Álvaro A. B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6513086/
https://www.ncbi.nlm.nih.gov/pubmed/31083695
http://dx.doi.org/10.1371/journal.pone.0216627
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author Macedo, Carlos Eduardo S.
da Conti, Guilherme
Catena, Andriu S.
Bruneska, Danyelly
Rosa, Malu
Noronha, Clarissa G.
Santa Cruz, Fernando
Ferraz, Álvaro A. B.
author_facet Macedo, Carlos Eduardo S.
da Conti, Guilherme
Catena, Andriu S.
Bruneska, Danyelly
Rosa, Malu
Noronha, Clarissa G.
Santa Cruz, Fernando
Ferraz, Álvaro A. B.
author_sort Macedo, Carlos Eduardo S.
collection PubMed
description OBJECTIVE: To assess the influence of bariatric surgery on transcription factor 7-like 2 (TCF7L2) expression and its association with body mass index (BMI) and Type 2 diabetes mellitus (T2DM). METHODS: Prospective study performed between 2016 and 2018, where 26 obese patients undergoing bariatric surgery were divided into two subgroups: diabetics and non-diabetics. The RNAs were extracted from peripheral blood samples that were obtained from each patient in two different moments: before surgery and after 12 months of follow-up. The relative expression of TCF7L2 was determined according to the delta-Ct method. RESULTS: The linear regression model of BMI x delta-Ct showed a positive correlation (p = 0.037). In the subgroups, an inversely proportional relationship was found between delta-Ct and BMI in the diabetic group and a directly proportional relationship in the non-diabetic group (p>0.05 in both). In the postoperative period, the regression model was similar to the preoperative, except when analyzing the subgroups, where diabetic patients showed a directly proportional relationship (p>0.05). The relative expression of TCF7L2 showed an average of 1.16 ± 0.91, CI-95% 0.79–1.53. There was an increase in relative expression of 48% in the non-diabetic group (p = 0.021), and a decrease of 27% in the T2DM group (p>0.05) in the postoperative. There was a positive correlation between a greater decrease in BMI and increased relative expression (p = 0.027). CONCLUSION: Our results showed that generally, the TCF7L2 expression increase with a decrease in BMI, however, for patients with T2DM, it exhibits an inverse pattern, which is normalized one year after bariatric surgery.
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spelling pubmed-65130862019-05-31 Assessment of TCF7L2 expression after bariatric surgery Macedo, Carlos Eduardo S. da Conti, Guilherme Catena, Andriu S. Bruneska, Danyelly Rosa, Malu Noronha, Clarissa G. Santa Cruz, Fernando Ferraz, Álvaro A. B. PLoS One Research Article OBJECTIVE: To assess the influence of bariatric surgery on transcription factor 7-like 2 (TCF7L2) expression and its association with body mass index (BMI) and Type 2 diabetes mellitus (T2DM). METHODS: Prospective study performed between 2016 and 2018, where 26 obese patients undergoing bariatric surgery were divided into two subgroups: diabetics and non-diabetics. The RNAs were extracted from peripheral blood samples that were obtained from each patient in two different moments: before surgery and after 12 months of follow-up. The relative expression of TCF7L2 was determined according to the delta-Ct method. RESULTS: The linear regression model of BMI x delta-Ct showed a positive correlation (p = 0.037). In the subgroups, an inversely proportional relationship was found between delta-Ct and BMI in the diabetic group and a directly proportional relationship in the non-diabetic group (p>0.05 in both). In the postoperative period, the regression model was similar to the preoperative, except when analyzing the subgroups, where diabetic patients showed a directly proportional relationship (p>0.05). The relative expression of TCF7L2 showed an average of 1.16 ± 0.91, CI-95% 0.79–1.53. There was an increase in relative expression of 48% in the non-diabetic group (p = 0.021), and a decrease of 27% in the T2DM group (p>0.05) in the postoperative. There was a positive correlation between a greater decrease in BMI and increased relative expression (p = 0.027). CONCLUSION: Our results showed that generally, the TCF7L2 expression increase with a decrease in BMI, however, for patients with T2DM, it exhibits an inverse pattern, which is normalized one year after bariatric surgery. Public Library of Science 2019-05-13 /pmc/articles/PMC6513086/ /pubmed/31083695 http://dx.doi.org/10.1371/journal.pone.0216627 Text en © 2019 Macedo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Macedo, Carlos Eduardo S.
da Conti, Guilherme
Catena, Andriu S.
Bruneska, Danyelly
Rosa, Malu
Noronha, Clarissa G.
Santa Cruz, Fernando
Ferraz, Álvaro A. B.
Assessment of TCF7L2 expression after bariatric surgery
title Assessment of TCF7L2 expression after bariatric surgery
title_full Assessment of TCF7L2 expression after bariatric surgery
title_fullStr Assessment of TCF7L2 expression after bariatric surgery
title_full_unstemmed Assessment of TCF7L2 expression after bariatric surgery
title_short Assessment of TCF7L2 expression after bariatric surgery
title_sort assessment of tcf7l2 expression after bariatric surgery
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6513086/
https://www.ncbi.nlm.nih.gov/pubmed/31083695
http://dx.doi.org/10.1371/journal.pone.0216627
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