Cargando…
Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer
Many bacterial pathogens can cause septicemia and spread from the bloodstream into internal organs. During leptospirosis, individuals are infected by contact with Leptospira-containing animal urine-contaminated water. The spirochetes invade internal organs after septicemia to cause disease aggravati...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6513555/ https://www.ncbi.nlm.nih.gov/pubmed/31012847 http://dx.doi.org/10.7554/eLife.44594 |
_version_ | 1783417751378853888 |
---|---|
author | Li, Yang Li, Kai-Xuan Hu, Wei-Lin Ojcius, David M Fang, Jia-Qi Li, Shi-Jun Lin, Xu'ai Yan, Jie |
author_facet | Li, Yang Li, Kai-Xuan Hu, Wei-Lin Ojcius, David M Fang, Jia-Qi Li, Shi-Jun Lin, Xu'ai Yan, Jie |
author_sort | Li, Yang |
collection | PubMed |
description | Many bacterial pathogens can cause septicemia and spread from the bloodstream into internal organs. During leptospirosis, individuals are infected by contact with Leptospira-containing animal urine-contaminated water. The spirochetes invade internal organs after septicemia to cause disease aggravation, but the mechanism of leptospiral excretion and spreading remains unknown. Here, we demonstrated that Leptospira interrogans entered human/mouse endothelial and epithelial cells and fibroblasts by caveolae/integrin-β1-PI3K/FAK-mediated microfilament-dependent endocytosis to form Leptospira (Lep)-vesicles that did not fuse with lysosomes. Lep-vesicles recruited Rab5/Rab11 and Sec/Exo-SNARE proteins in endocytic recycling and vesicular transport systems for intracellular transport and release by SNARE-complex/FAK-mediated microfilament/microtubule-dependent exocytosis. Both intracellular leptospires and infected cells maintained their viability. Leptospiral propagation was only observed in mouse fibroblasts. Our study revealed that L. interrogans utilizes endocytic recycling and vesicular transport systems for transcytosis across endothelial or epithelial barrier in blood vessels or renal tubules, which contributes to spreading in vivo and transmission of leptospirosis. |
format | Online Article Text |
id | pubmed-6513555 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-65135552019-05-16 Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer Li, Yang Li, Kai-Xuan Hu, Wei-Lin Ojcius, David M Fang, Jia-Qi Li, Shi-Jun Lin, Xu'ai Yan, Jie eLife Microbiology and Infectious Disease Many bacterial pathogens can cause septicemia and spread from the bloodstream into internal organs. During leptospirosis, individuals are infected by contact with Leptospira-containing animal urine-contaminated water. The spirochetes invade internal organs after septicemia to cause disease aggravation, but the mechanism of leptospiral excretion and spreading remains unknown. Here, we demonstrated that Leptospira interrogans entered human/mouse endothelial and epithelial cells and fibroblasts by caveolae/integrin-β1-PI3K/FAK-mediated microfilament-dependent endocytosis to form Leptospira (Lep)-vesicles that did not fuse with lysosomes. Lep-vesicles recruited Rab5/Rab11 and Sec/Exo-SNARE proteins in endocytic recycling and vesicular transport systems for intracellular transport and release by SNARE-complex/FAK-mediated microfilament/microtubule-dependent exocytosis. Both intracellular leptospires and infected cells maintained their viability. Leptospiral propagation was only observed in mouse fibroblasts. Our study revealed that L. interrogans utilizes endocytic recycling and vesicular transport systems for transcytosis across endothelial or epithelial barrier in blood vessels or renal tubules, which contributes to spreading in vivo and transmission of leptospirosis. eLife Sciences Publications, Ltd 2019-04-23 /pmc/articles/PMC6513555/ /pubmed/31012847 http://dx.doi.org/10.7554/eLife.44594 Text en © 2019, Li et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Microbiology and Infectious Disease Li, Yang Li, Kai-Xuan Hu, Wei-Lin Ojcius, David M Fang, Jia-Qi Li, Shi-Jun Lin, Xu'ai Yan, Jie Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer |
title | Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer |
title_full | Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer |
title_fullStr | Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer |
title_full_unstemmed | Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer |
title_short | Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer |
title_sort | endocytic recycling and vesicular transport systems mediate transcytosis of leptospira interrogans across cell monolayer |
topic | Microbiology and Infectious Disease |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6513555/ https://www.ncbi.nlm.nih.gov/pubmed/31012847 http://dx.doi.org/10.7554/eLife.44594 |
work_keys_str_mv | AT liyang endocyticrecyclingandvesiculartransportsystemsmediatetranscytosisofleptospirainterrogansacrosscellmonolayer AT likaixuan endocyticrecyclingandvesiculartransportsystemsmediatetranscytosisofleptospirainterrogansacrosscellmonolayer AT huweilin endocyticrecyclingandvesiculartransportsystemsmediatetranscytosisofleptospirainterrogansacrosscellmonolayer AT ojciusdavidm endocyticrecyclingandvesiculartransportsystemsmediatetranscytosisofleptospirainterrogansacrosscellmonolayer AT fangjiaqi endocyticrecyclingandvesiculartransportsystemsmediatetranscytosisofleptospirainterrogansacrosscellmonolayer AT lishijun endocyticrecyclingandvesiculartransportsystemsmediatetranscytosisofleptospirainterrogansacrosscellmonolayer AT linxuai endocyticrecyclingandvesiculartransportsystemsmediatetranscytosisofleptospirainterrogansacrosscellmonolayer AT yanjie endocyticrecyclingandvesiculartransportsystemsmediatetranscytosisofleptospirainterrogansacrosscellmonolayer |