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Protective effects of glycerol and xylitol in keratinocytes exposed to hyperosmotic stress

Purpose: Our goal was to study whether glycerol and xylitol provide protection against osmotic stress in keratinocytes. Methods: The experiments were performed on HaCaT keratinocytes. Hyperosmotic stress was induced by the addition of sorbitol (450, 500 and 600 mOsm). Both polyols were applied at tw...

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Autores principales: Szél, Edit, Danis, Judit, Sőrés, Evelin, Tóth, Dániel, Korponyai, Csilla, Degovics, Döníz, Prorok, János, Acsai, Károly, Dikstein, Shabtay, Kemény, Lajos, Erős, Gábor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6514140/
https://www.ncbi.nlm.nih.gov/pubmed/31190939
http://dx.doi.org/10.2147/CCID.S197946
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author Szél, Edit
Danis, Judit
Sőrés, Evelin
Tóth, Dániel
Korponyai, Csilla
Degovics, Döníz
Prorok, János
Acsai, Károly
Dikstein, Shabtay
Kemény, Lajos
Erős, Gábor
author_facet Szél, Edit
Danis, Judit
Sőrés, Evelin
Tóth, Dániel
Korponyai, Csilla
Degovics, Döníz
Prorok, János
Acsai, Károly
Dikstein, Shabtay
Kemény, Lajos
Erős, Gábor
author_sort Szél, Edit
collection PubMed
description Purpose: Our goal was to study whether glycerol and xylitol provide protection against osmotic stress in keratinocytes. Methods: The experiments were performed on HaCaT keratinocytes. Hyperosmotic stress was induced by the addition of sorbitol (450, 500 and 600 mOsm). Both polyols were applied at two different concentrations (glycerol: 0.027% and 0.27%, xylitol: 0.045% and 0.45%). Cellular viability and cytotoxicity were assessed, intracellular Ca(2+) concentration was measured, and the RNA expression of inflammatory cytokines was determined by means of PCR. Differences among groups were analyzed with one-way ANOVA and Holm-Sidak post-hoc test. When the normality test failed, Kruskal–Wallis one-way analysis of variance on ranks, followed by Dunn’s method for pairwise multiple comparison was performed. Results: The higher concentrations of the polyols were effective. Glycerol ameliorated the cellular viability while xylitol prevented the rapid Ca(2+) signal. Both polyols suppressed the expression of IL-1α but only glycerol decreased the expression of IL-1β and NFAT5. Conclusions: Glycerol and xylitol protect keratinocytes against osmotic stress. Despite their similar chemical structure, the effect of these polyols displayed differences. Hence, joint application of glycerol and xylitol may be a useful therapeutic approach for different skin disorders.
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spelling pubmed-65141402019-06-12 Protective effects of glycerol and xylitol in keratinocytes exposed to hyperosmotic stress Szél, Edit Danis, Judit Sőrés, Evelin Tóth, Dániel Korponyai, Csilla Degovics, Döníz Prorok, János Acsai, Károly Dikstein, Shabtay Kemény, Lajos Erős, Gábor Clin Cosmet Investig Dermatol Original Research Purpose: Our goal was to study whether glycerol and xylitol provide protection against osmotic stress in keratinocytes. Methods: The experiments were performed on HaCaT keratinocytes. Hyperosmotic stress was induced by the addition of sorbitol (450, 500 and 600 mOsm). Both polyols were applied at two different concentrations (glycerol: 0.027% and 0.27%, xylitol: 0.045% and 0.45%). Cellular viability and cytotoxicity were assessed, intracellular Ca(2+) concentration was measured, and the RNA expression of inflammatory cytokines was determined by means of PCR. Differences among groups were analyzed with one-way ANOVA and Holm-Sidak post-hoc test. When the normality test failed, Kruskal–Wallis one-way analysis of variance on ranks, followed by Dunn’s method for pairwise multiple comparison was performed. Results: The higher concentrations of the polyols were effective. Glycerol ameliorated the cellular viability while xylitol prevented the rapid Ca(2+) signal. Both polyols suppressed the expression of IL-1α but only glycerol decreased the expression of IL-1β and NFAT5. Conclusions: Glycerol and xylitol protect keratinocytes against osmotic stress. Despite their similar chemical structure, the effect of these polyols displayed differences. Hence, joint application of glycerol and xylitol may be a useful therapeutic approach for different skin disorders. Dove 2019-05-08 /pmc/articles/PMC6514140/ /pubmed/31190939 http://dx.doi.org/10.2147/CCID.S197946 Text en © 2019 Szél et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Szél, Edit
Danis, Judit
Sőrés, Evelin
Tóth, Dániel
Korponyai, Csilla
Degovics, Döníz
Prorok, János
Acsai, Károly
Dikstein, Shabtay
Kemény, Lajos
Erős, Gábor
Protective effects of glycerol and xylitol in keratinocytes exposed to hyperosmotic stress
title Protective effects of glycerol and xylitol in keratinocytes exposed to hyperosmotic stress
title_full Protective effects of glycerol and xylitol in keratinocytes exposed to hyperosmotic stress
title_fullStr Protective effects of glycerol and xylitol in keratinocytes exposed to hyperosmotic stress
title_full_unstemmed Protective effects of glycerol and xylitol in keratinocytes exposed to hyperosmotic stress
title_short Protective effects of glycerol and xylitol in keratinocytes exposed to hyperosmotic stress
title_sort protective effects of glycerol and xylitol in keratinocytes exposed to hyperosmotic stress
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6514140/
https://www.ncbi.nlm.nih.gov/pubmed/31190939
http://dx.doi.org/10.2147/CCID.S197946
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