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The Content of Extracellular Nucleic Acids in the Blood and Ejaculate of Men of Reproductive Age Living in the Ecologically Unfavourable Regions of the Aral Sea

AIM: We aimed to study the influence of adverse environmental factors on molecular-cellular processes in the population living in the Aral Sea region. METHODS: Extracellular RNA (ecRNA) and ecDNA were determined in blood. We obtained the ejaculate of the studied men after 4-5 days of abstinence. The...

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Autores principales: Jangildinova, Svetlana, Tatina, Yelena, Kaliyeva, Gulzhan, Kuvatbaeva, Kuttykyz, Beygam, Nursaya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Republic of Macedonia 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6514334/
https://www.ncbi.nlm.nih.gov/pubmed/31110590
http://dx.doi.org/10.3889/oamjms.2019.273
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author Jangildinova, Svetlana
Tatina, Yelena
Kaliyeva, Gulzhan
Kuvatbaeva, Kuttykyz
Beygam, Nursaya
author_facet Jangildinova, Svetlana
Tatina, Yelena
Kaliyeva, Gulzhan
Kuvatbaeva, Kuttykyz
Beygam, Nursaya
author_sort Jangildinova, Svetlana
collection PubMed
description AIM: We aimed to study the influence of adverse environmental factors on molecular-cellular processes in the population living in the Aral Sea region. METHODS: Extracellular RNA (ecRNA) and ecDNA were determined in blood. We obtained the ejaculate of the studied men after 4-5 days of abstinence. The ejaculate was placed in a warm tube with a ground stopper. The examination of the ejaculate was started in 20-30 minutes after receiving, as during this time it is subjected to liquefaction. Spectrophotometry of ASF, RNA and DNA hydrolysates was performed on an SF 26 at a wavelength of 290 nm against H(2)O. RESULTS: In the ejaculate of the studied groups of men, significant deviations in the content of extracellular nucleic acid fractions from the indicators of the comparison group were also detected. Statistically significant differences in the content of extracellular RNA were observed in men of the younger age group living in the territory of all study regions. A significant increase in the content of extracellular DNA was detected in two regions, but not in all age groups. CONCLUSION: The study revealed a significant increase in the content of extracellular nucleic acids in the biological fluids of men of reproductive age living in the Aral Sea region. The most significant are the changes in the level of extracellular RNA in the blood plasma and ejaculate in men of the younger age group and the increase in ASF content in the ejaculate in men of all age groups.
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spelling pubmed-65143342019-05-20 The Content of Extracellular Nucleic Acids in the Blood and Ejaculate of Men of Reproductive Age Living in the Ecologically Unfavourable Regions of the Aral Sea Jangildinova, Svetlana Tatina, Yelena Kaliyeva, Gulzhan Kuvatbaeva, Kuttykyz Beygam, Nursaya Open Access Maced J Med Sci Public Health AIM: We aimed to study the influence of adverse environmental factors on molecular-cellular processes in the population living in the Aral Sea region. METHODS: Extracellular RNA (ecRNA) and ecDNA were determined in blood. We obtained the ejaculate of the studied men after 4-5 days of abstinence. The ejaculate was placed in a warm tube with a ground stopper. The examination of the ejaculate was started in 20-30 minutes after receiving, as during this time it is subjected to liquefaction. Spectrophotometry of ASF, RNA and DNA hydrolysates was performed on an SF 26 at a wavelength of 290 nm against H(2)O. RESULTS: In the ejaculate of the studied groups of men, significant deviations in the content of extracellular nucleic acid fractions from the indicators of the comparison group were also detected. Statistically significant differences in the content of extracellular RNA were observed in men of the younger age group living in the territory of all study regions. A significant increase in the content of extracellular DNA was detected in two regions, but not in all age groups. CONCLUSION: The study revealed a significant increase in the content of extracellular nucleic acids in the biological fluids of men of reproductive age living in the Aral Sea region. The most significant are the changes in the level of extracellular RNA in the blood plasma and ejaculate in men of the younger age group and the increase in ASF content in the ejaculate in men of all age groups. Republic of Macedonia 2019-04-28 /pmc/articles/PMC6514334/ /pubmed/31110590 http://dx.doi.org/10.3889/oamjms.2019.273 Text en Copyright: © 2019 Svetlana Jangildinova, Yelena Tatina, Gulzhan Kaliyeva, Kuttykyz Kuvatbaeva, Nursaya Beygam. http://creativecommons.org/licenses/CC BY-NC/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
spellingShingle Public Health
Jangildinova, Svetlana
Tatina, Yelena
Kaliyeva, Gulzhan
Kuvatbaeva, Kuttykyz
Beygam, Nursaya
The Content of Extracellular Nucleic Acids in the Blood and Ejaculate of Men of Reproductive Age Living in the Ecologically Unfavourable Regions of the Aral Sea
title The Content of Extracellular Nucleic Acids in the Blood and Ejaculate of Men of Reproductive Age Living in the Ecologically Unfavourable Regions of the Aral Sea
title_full The Content of Extracellular Nucleic Acids in the Blood and Ejaculate of Men of Reproductive Age Living in the Ecologically Unfavourable Regions of the Aral Sea
title_fullStr The Content of Extracellular Nucleic Acids in the Blood and Ejaculate of Men of Reproductive Age Living in the Ecologically Unfavourable Regions of the Aral Sea
title_full_unstemmed The Content of Extracellular Nucleic Acids in the Blood and Ejaculate of Men of Reproductive Age Living in the Ecologically Unfavourable Regions of the Aral Sea
title_short The Content of Extracellular Nucleic Acids in the Blood and Ejaculate of Men of Reproductive Age Living in the Ecologically Unfavourable Regions of the Aral Sea
title_sort content of extracellular nucleic acids in the blood and ejaculate of men of reproductive age living in the ecologically unfavourable regions of the aral sea
topic Public Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6514334/
https://www.ncbi.nlm.nih.gov/pubmed/31110590
http://dx.doi.org/10.3889/oamjms.2019.273
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