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Laccase Immobilized onto Zirconia–Silica Hybrid Doped with Cu(2+) as an Effective Biocatalytic System for Decolorization of Dyes
Nowadays, novel and advanced methods are being sought to efficiently remove dyes from wastewaters. These compounds, which mainly originate from the textile industry, may adversely affect the aquatic environment as well as living organisms. Thus, in presented study, the synthesized ZrO(2)–SiO(2) and...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6514565/ https://www.ncbi.nlm.nih.gov/pubmed/30995753 http://dx.doi.org/10.3390/ma12081252 |
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author | Jankowska, Katarzyna Ciesielczyk, Filip Bachosz, Karolina Zdarta, Jakub Kaczorek, Ewa Jesionowski, Teofil |
author_facet | Jankowska, Katarzyna Ciesielczyk, Filip Bachosz, Karolina Zdarta, Jakub Kaczorek, Ewa Jesionowski, Teofil |
author_sort | Jankowska, Katarzyna |
collection | PubMed |
description | Nowadays, novel and advanced methods are being sought to efficiently remove dyes from wastewaters. These compounds, which mainly originate from the textile industry, may adversely affect the aquatic environment as well as living organisms. Thus, in presented study, the synthesized ZrO(2)–SiO(2) and Cu(2+)-doped ZrO(2)–SiO(2) oxide materials were used for the first time as supports for laccase immobilization, which was carried out for 1 h, at pH 5 and 25 °C. The materials were thoroughly characterized before and after laccase immobilization with respect to electrokinetic stability, parameters of the porous structure, morphology and type of surface functional groups. Additionally, the immobilization yields were defined, which reached 86% and 94% for ZrO(2)–SiO(2)–laccase and ZrO(2)–SiO(2)/Cu(2+)–laccase, respectively. Furthermore, the obtained biocatalytic systems were used for enzymatic decolorization of the Remazol Brilliant Blue R (RBBR) dye from model aqueous solutions, under various reaction conditions (time, temperature, pH). The best conditions of the decolorization process (24 h, 30 °C and pH = 4) allowed to achieve the highest decolorization efficiencies of 98% and 90% for ZrO(2)–SiO(2)–laccase and ZrO(2)–SiO(2)/Cu(2+)–laccase, respectively. Finally, it was established that the mortality of Artemia salina in solutions after enzymatic decolorization was lower by approx. 20% and 30% for ZrO(2)–SiO(2)–laccase and ZrO(2)–SiO(2)/Cu(2+)–laccase, respectively, as compared to the solution before enzymatic treatment, which indicated lower toxicity of the solution. Thus, it should be clearly stated that doping of the oxide support with copper ions positively affects enzyme stability, activity and, in consequence, the removal efficiency of the RBBR dye. |
format | Online Article Text |
id | pubmed-6514565 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65145652019-05-31 Laccase Immobilized onto Zirconia–Silica Hybrid Doped with Cu(2+) as an Effective Biocatalytic System for Decolorization of Dyes Jankowska, Katarzyna Ciesielczyk, Filip Bachosz, Karolina Zdarta, Jakub Kaczorek, Ewa Jesionowski, Teofil Materials (Basel) Article Nowadays, novel and advanced methods are being sought to efficiently remove dyes from wastewaters. These compounds, which mainly originate from the textile industry, may adversely affect the aquatic environment as well as living organisms. Thus, in presented study, the synthesized ZrO(2)–SiO(2) and Cu(2+)-doped ZrO(2)–SiO(2) oxide materials were used for the first time as supports for laccase immobilization, which was carried out for 1 h, at pH 5 and 25 °C. The materials were thoroughly characterized before and after laccase immobilization with respect to electrokinetic stability, parameters of the porous structure, morphology and type of surface functional groups. Additionally, the immobilization yields were defined, which reached 86% and 94% for ZrO(2)–SiO(2)–laccase and ZrO(2)–SiO(2)/Cu(2+)–laccase, respectively. Furthermore, the obtained biocatalytic systems were used for enzymatic decolorization of the Remazol Brilliant Blue R (RBBR) dye from model aqueous solutions, under various reaction conditions (time, temperature, pH). The best conditions of the decolorization process (24 h, 30 °C and pH = 4) allowed to achieve the highest decolorization efficiencies of 98% and 90% for ZrO(2)–SiO(2)–laccase and ZrO(2)–SiO(2)/Cu(2+)–laccase, respectively. Finally, it was established that the mortality of Artemia salina in solutions after enzymatic decolorization was lower by approx. 20% and 30% for ZrO(2)–SiO(2)–laccase and ZrO(2)–SiO(2)/Cu(2+)–laccase, respectively, as compared to the solution before enzymatic treatment, which indicated lower toxicity of the solution. Thus, it should be clearly stated that doping of the oxide support with copper ions positively affects enzyme stability, activity and, in consequence, the removal efficiency of the RBBR dye. MDPI 2019-04-16 /pmc/articles/PMC6514565/ /pubmed/30995753 http://dx.doi.org/10.3390/ma12081252 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jankowska, Katarzyna Ciesielczyk, Filip Bachosz, Karolina Zdarta, Jakub Kaczorek, Ewa Jesionowski, Teofil Laccase Immobilized onto Zirconia–Silica Hybrid Doped with Cu(2+) as an Effective Biocatalytic System for Decolorization of Dyes |
title | Laccase Immobilized onto Zirconia–Silica Hybrid Doped with Cu(2+) as an Effective Biocatalytic System for Decolorization of Dyes |
title_full | Laccase Immobilized onto Zirconia–Silica Hybrid Doped with Cu(2+) as an Effective Biocatalytic System for Decolorization of Dyes |
title_fullStr | Laccase Immobilized onto Zirconia–Silica Hybrid Doped with Cu(2+) as an Effective Biocatalytic System for Decolorization of Dyes |
title_full_unstemmed | Laccase Immobilized onto Zirconia–Silica Hybrid Doped with Cu(2+) as an Effective Biocatalytic System for Decolorization of Dyes |
title_short | Laccase Immobilized onto Zirconia–Silica Hybrid Doped with Cu(2+) as an Effective Biocatalytic System for Decolorization of Dyes |
title_sort | laccase immobilized onto zirconia–silica hybrid doped with cu(2+) as an effective biocatalytic system for decolorization of dyes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6514565/ https://www.ncbi.nlm.nih.gov/pubmed/30995753 http://dx.doi.org/10.3390/ma12081252 |
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