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High-Throughput Biochemical Fingerprinting of Oleaginous Aurantiochytrium sp. Strains by Fourier Transform Infrared Spectroscopy (FT-IR) for Lipid and Carbohydrate Productions

The traditional biochemical methods for analyzing cellular composition of oleaginous microorganisms are time-consuming, polluting, and expensive. In the present study, an FT-IR method was used to analyze the cellular composition of the marine oleaginous protist Aurantiochytrium sp. during various re...

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Autores principales: Yu, Xin-Jun, Huang, Chang-Yi, Chen, Hong, Wang, Dong-Sheng, Chen, Jing-Liang, Li, Hui-Juan, Liu, Xiao-Yan, Wang, Zhao, Sun, Jie, Wang, Zhi-Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6514702/
https://www.ncbi.nlm.nih.gov/pubmed/31013676
http://dx.doi.org/10.3390/molecules24081593
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author Yu, Xin-Jun
Huang, Chang-Yi
Chen, Hong
Wang, Dong-Sheng
Chen, Jing-Liang
Li, Hui-Juan
Liu, Xiao-Yan
Wang, Zhao
Sun, Jie
Wang, Zhi-Peng
author_facet Yu, Xin-Jun
Huang, Chang-Yi
Chen, Hong
Wang, Dong-Sheng
Chen, Jing-Liang
Li, Hui-Juan
Liu, Xiao-Yan
Wang, Zhao
Sun, Jie
Wang, Zhi-Peng
author_sort Yu, Xin-Jun
collection PubMed
description The traditional biochemical methods for analyzing cellular composition of oleaginous microorganisms are time-consuming, polluting, and expensive. In the present study, an FT-IR method was used to analyze the cellular composition of the marine oleaginous protist Aurantiochytrium sp. during various research processes, such as strains screening, medium optimization, and fermentation, and was evaluated as a green, low-cost, high throughput, and accurate method compared with the traditional methods. A total of 109 Aurantiochytrium sp. strains were screened for lipid and carbohydrate production and the best results were found for the strains No. 6 and No. 32. The yields and productivities could reach up to 47.2 g/L and 0.72 g/L/h for lipid, 21.6 g/L and 0.33 g/L/h for docosahexaenoic acid (DHA) in the strain No. 6, and 15.4 g/L and 0.18 g/L/h for carbohydrate in the strain No. 32, under the optimal conditions, respectively. These results confirmed potentials of the two Aurantiochytrium sp. strains for lipid, DHA, and carbohydrate productions at industrial scales. The FT-IR method in this study will facilitate research on the oleaginous Aurantiochytrium sp., and the obtained two strains for lipid and carbohydrate productions will provide the foundations for their applications in medical, food, and feed industries.
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spelling pubmed-65147022019-05-30 High-Throughput Biochemical Fingerprinting of Oleaginous Aurantiochytrium sp. Strains by Fourier Transform Infrared Spectroscopy (FT-IR) for Lipid and Carbohydrate Productions Yu, Xin-Jun Huang, Chang-Yi Chen, Hong Wang, Dong-Sheng Chen, Jing-Liang Li, Hui-Juan Liu, Xiao-Yan Wang, Zhao Sun, Jie Wang, Zhi-Peng Molecules Article The traditional biochemical methods for analyzing cellular composition of oleaginous microorganisms are time-consuming, polluting, and expensive. In the present study, an FT-IR method was used to analyze the cellular composition of the marine oleaginous protist Aurantiochytrium sp. during various research processes, such as strains screening, medium optimization, and fermentation, and was evaluated as a green, low-cost, high throughput, and accurate method compared with the traditional methods. A total of 109 Aurantiochytrium sp. strains were screened for lipid and carbohydrate production and the best results were found for the strains No. 6 and No. 32. The yields and productivities could reach up to 47.2 g/L and 0.72 g/L/h for lipid, 21.6 g/L and 0.33 g/L/h for docosahexaenoic acid (DHA) in the strain No. 6, and 15.4 g/L and 0.18 g/L/h for carbohydrate in the strain No. 32, under the optimal conditions, respectively. These results confirmed potentials of the two Aurantiochytrium sp. strains for lipid, DHA, and carbohydrate productions at industrial scales. The FT-IR method in this study will facilitate research on the oleaginous Aurantiochytrium sp., and the obtained two strains for lipid and carbohydrate productions will provide the foundations for their applications in medical, food, and feed industries. MDPI 2019-04-22 /pmc/articles/PMC6514702/ /pubmed/31013676 http://dx.doi.org/10.3390/molecules24081593 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yu, Xin-Jun
Huang, Chang-Yi
Chen, Hong
Wang, Dong-Sheng
Chen, Jing-Liang
Li, Hui-Juan
Liu, Xiao-Yan
Wang, Zhao
Sun, Jie
Wang, Zhi-Peng
High-Throughput Biochemical Fingerprinting of Oleaginous Aurantiochytrium sp. Strains by Fourier Transform Infrared Spectroscopy (FT-IR) for Lipid and Carbohydrate Productions
title High-Throughput Biochemical Fingerprinting of Oleaginous Aurantiochytrium sp. Strains by Fourier Transform Infrared Spectroscopy (FT-IR) for Lipid and Carbohydrate Productions
title_full High-Throughput Biochemical Fingerprinting of Oleaginous Aurantiochytrium sp. Strains by Fourier Transform Infrared Spectroscopy (FT-IR) for Lipid and Carbohydrate Productions
title_fullStr High-Throughput Biochemical Fingerprinting of Oleaginous Aurantiochytrium sp. Strains by Fourier Transform Infrared Spectroscopy (FT-IR) for Lipid and Carbohydrate Productions
title_full_unstemmed High-Throughput Biochemical Fingerprinting of Oleaginous Aurantiochytrium sp. Strains by Fourier Transform Infrared Spectroscopy (FT-IR) for Lipid and Carbohydrate Productions
title_short High-Throughput Biochemical Fingerprinting of Oleaginous Aurantiochytrium sp. Strains by Fourier Transform Infrared Spectroscopy (FT-IR) for Lipid and Carbohydrate Productions
title_sort high-throughput biochemical fingerprinting of oleaginous aurantiochytrium sp. strains by fourier transform infrared spectroscopy (ft-ir) for lipid and carbohydrate productions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6514702/
https://www.ncbi.nlm.nih.gov/pubmed/31013676
http://dx.doi.org/10.3390/molecules24081593
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