Cargando…

Sex differences in cardiac and renal responses to a high salt diet in Sprague-Dawley rats

High dietary salt intake is an important risk factor for cardiovascular and renal diseases. However, sexual disparity exists in the response of target organs to high salt diet (HSD). To determine how sex affects cardiac and renal functions’ response to HSD, 20 weanling Sprague-Dawley rats (10 males...

Descripción completa

Detalles Bibliográficos
Autores principales: Oloyo, Ahmed Kolade, Imaga, Ngozi O.A., Fatope, Yemisi, Sofola, Olusoga A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6514751/
https://www.ncbi.nlm.nih.gov/pubmed/31193051
http://dx.doi.org/10.1016/j.heliyon.2019.e01665
_version_ 1783417933611925504
author Oloyo, Ahmed Kolade
Imaga, Ngozi O.A.
Fatope, Yemisi
Sofola, Olusoga A.
author_facet Oloyo, Ahmed Kolade
Imaga, Ngozi O.A.
Fatope, Yemisi
Sofola, Olusoga A.
author_sort Oloyo, Ahmed Kolade
collection PubMed
description High dietary salt intake is an important risk factor for cardiovascular and renal diseases. However, sexual disparity exists in the response of target organs to high salt diet (HSD). To determine how sex affects cardiac and renal functions’ response to HSD, 20 weanling Sprague-Dawley rats (10 males and 10 females) were divided into 4 groups of 5 rats each. The rats were fed a normal diet (0.3% NaCl) or HSD (8% NaCl) for 12 weeks. Fluid balance (FB) was determined from 24 hrs water intake and voided urine. Blood pressure (BP) was measured via arterial cannulation under anesthesia (25% w/v urethane and 1% w/v α-chloralose; 5 ml/kg, i.p). Serum levels of troponin I, aminotransaminases, creatinine, urea, uric acid and electrolytes as well as urinary concentration of albumin, creatinine, and electrolytes were measured using appropriate assay kits. Values are presented as mean ± S.E.M, compared by two-way ANOVA and Bonferroni post Hoc test. In the male rat, HSD significantly increased BP, serum: Troponin I, LDH and sodium (p < 0.05), urinary: albumin, sodium, potassium and FB (p < 0.05). In the female rat, HSD increased BP, serum: troponin I, LDH, sodium and creatinine clearance (p < 0.05), urinary: albumin, sodium and potassium (p < 0.01). However, HSD increased more, the BP, serum: Troponin I, LDH, urinary albumin and FB in male rats, while HSD increased urinary sodium more in female rats. Basal values in male vs. female of serum LDH and urinary albumin were significantly different. Thus, sex plays an important role in the response of the heart and kidney to salt stress.
format Online
Article
Text
id pubmed-6514751
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-65147512019-05-20 Sex differences in cardiac and renal responses to a high salt diet in Sprague-Dawley rats Oloyo, Ahmed Kolade Imaga, Ngozi O.A. Fatope, Yemisi Sofola, Olusoga A. Heliyon Article High dietary salt intake is an important risk factor for cardiovascular and renal diseases. However, sexual disparity exists in the response of target organs to high salt diet (HSD). To determine how sex affects cardiac and renal functions’ response to HSD, 20 weanling Sprague-Dawley rats (10 males and 10 females) were divided into 4 groups of 5 rats each. The rats were fed a normal diet (0.3% NaCl) or HSD (8% NaCl) for 12 weeks. Fluid balance (FB) was determined from 24 hrs water intake and voided urine. Blood pressure (BP) was measured via arterial cannulation under anesthesia (25% w/v urethane and 1% w/v α-chloralose; 5 ml/kg, i.p). Serum levels of troponin I, aminotransaminases, creatinine, urea, uric acid and electrolytes as well as urinary concentration of albumin, creatinine, and electrolytes were measured using appropriate assay kits. Values are presented as mean ± S.E.M, compared by two-way ANOVA and Bonferroni post Hoc test. In the male rat, HSD significantly increased BP, serum: Troponin I, LDH and sodium (p < 0.05), urinary: albumin, sodium, potassium and FB (p < 0.05). In the female rat, HSD increased BP, serum: troponin I, LDH, sodium and creatinine clearance (p < 0.05), urinary: albumin, sodium and potassium (p < 0.01). However, HSD increased more, the BP, serum: Troponin I, LDH, urinary albumin and FB in male rats, while HSD increased urinary sodium more in female rats. Basal values in male vs. female of serum LDH and urinary albumin were significantly different. Thus, sex plays an important role in the response of the heart and kidney to salt stress. Elsevier 2019-05-09 /pmc/articles/PMC6514751/ /pubmed/31193051 http://dx.doi.org/10.1016/j.heliyon.2019.e01665 Text en © 2019 Published by Elsevier Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Oloyo, Ahmed Kolade
Imaga, Ngozi O.A.
Fatope, Yemisi
Sofola, Olusoga A.
Sex differences in cardiac and renal responses to a high salt diet in Sprague-Dawley rats
title Sex differences in cardiac and renal responses to a high salt diet in Sprague-Dawley rats
title_full Sex differences in cardiac and renal responses to a high salt diet in Sprague-Dawley rats
title_fullStr Sex differences in cardiac and renal responses to a high salt diet in Sprague-Dawley rats
title_full_unstemmed Sex differences in cardiac and renal responses to a high salt diet in Sprague-Dawley rats
title_short Sex differences in cardiac and renal responses to a high salt diet in Sprague-Dawley rats
title_sort sex differences in cardiac and renal responses to a high salt diet in sprague-dawley rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6514751/
https://www.ncbi.nlm.nih.gov/pubmed/31193051
http://dx.doi.org/10.1016/j.heliyon.2019.e01665
work_keys_str_mv AT oloyoahmedkolade sexdifferencesincardiacandrenalresponsestoahighsaltdietinspraguedawleyrats
AT imagangozioa sexdifferencesincardiacandrenalresponsestoahighsaltdietinspraguedawleyrats
AT fatopeyemisi sexdifferencesincardiacandrenalresponsestoahighsaltdietinspraguedawleyrats
AT sofolaolusogaa sexdifferencesincardiacandrenalresponsestoahighsaltdietinspraguedawleyrats