New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds

Thinopyrum has been widely used to improve wheat (Triticum aestivum L.) cultivars. Non-denaturing fluorescence in situ hybridization (ND-FISH) technology using oligonucleotides (oligo) as probes provides a convenient and efficient way to identify alien chromosomes in wheat backgrounds. However, suit...

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Autores principales: Xi, Wei, Tang, Zongxiang, Tang, Shuyao, Yang, Zujun, Luo, Jie, Fu, Shulan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6515231/
https://www.ncbi.nlm.nih.gov/pubmed/31027154
http://dx.doi.org/10.3390/ijms20082031
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author Xi, Wei
Tang, Zongxiang
Tang, Shuyao
Yang, Zujun
Luo, Jie
Fu, Shulan
author_facet Xi, Wei
Tang, Zongxiang
Tang, Shuyao
Yang, Zujun
Luo, Jie
Fu, Shulan
author_sort Xi, Wei
collection PubMed
description Thinopyrum has been widely used to improve wheat (Triticum aestivum L.) cultivars. Non-denaturing fluorescence in situ hybridization (ND-FISH) technology using oligonucleotides (oligo) as probes provides a convenient and efficient way to identify alien chromosomes in wheat backgrounds. However, suitable ND-FISH-positive oligo probes for distinguishing Thinopyrum chromosomes from wheat are lacking. Two oligo probes, Oligo-B11 and Oligo-pThp3.93, were designed according to the published Thinopyrum ponticum (Th. ponticum)-specific repetitive sequences. Both Oligo-B11 and Oligo-pThp3.93 can be used for ND-FISH analysis and can replace conventional GISH and FISH to discriminate some chromosomes of Th. elongatum, Th. intermedium, and Th. ponticum in wheat backgrounds. The two oligo probes provide a convenient way for the utilization of Thinopyrum germplasms in future wheat breeding programs.
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spelling pubmed-65152312019-05-30 New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds Xi, Wei Tang, Zongxiang Tang, Shuyao Yang, Zujun Luo, Jie Fu, Shulan Int J Mol Sci Brief Report Thinopyrum has been widely used to improve wheat (Triticum aestivum L.) cultivars. Non-denaturing fluorescence in situ hybridization (ND-FISH) technology using oligonucleotides (oligo) as probes provides a convenient and efficient way to identify alien chromosomes in wheat backgrounds. However, suitable ND-FISH-positive oligo probes for distinguishing Thinopyrum chromosomes from wheat are lacking. Two oligo probes, Oligo-B11 and Oligo-pThp3.93, were designed according to the published Thinopyrum ponticum (Th. ponticum)-specific repetitive sequences. Both Oligo-B11 and Oligo-pThp3.93 can be used for ND-FISH analysis and can replace conventional GISH and FISH to discriminate some chromosomes of Th. elongatum, Th. intermedium, and Th. ponticum in wheat backgrounds. The two oligo probes provide a convenient way for the utilization of Thinopyrum germplasms in future wheat breeding programs. MDPI 2019-04-25 /pmc/articles/PMC6515231/ /pubmed/31027154 http://dx.doi.org/10.3390/ijms20082031 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Xi, Wei
Tang, Zongxiang
Tang, Shuyao
Yang, Zujun
Luo, Jie
Fu, Shulan
New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds
title New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds
title_full New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds
title_fullStr New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds
title_full_unstemmed New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds
title_short New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds
title_sort new nd-fish-positive oligo probes for identifying thinopyrum chromosomes in wheat backgrounds
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6515231/
https://www.ncbi.nlm.nih.gov/pubmed/31027154
http://dx.doi.org/10.3390/ijms20082031
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