Cargando…
Diamond Nanoparticles Downregulate Expression of CycD and CycE in Glioma Cells
Our previous studies have shown that diamond nanoparticles (NDs) exhibited antiangiogenic and proapoptotic properties in vitro in glioblastoma multiforme (GBM) cells and in tumors in vivo. Moreover, NDs inhibited adhesion, leading to the suppression of migration and invasion of GBM. In the present s...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6515518/ https://www.ncbi.nlm.nih.gov/pubmed/31010146 http://dx.doi.org/10.3390/molecules24081549 |
_version_ | 1783418101748989952 |
---|---|
author | Grodzik, Marta Szczepaniak, Jaroslaw Strojny-Cieslak, Barbara Hotowy, Anna Wierzbicki, Mateusz Jaworski, Slawomir Kutwin, Marta Soltan, Emilia Mandat, Tomasz Lewicka, Aneta Chwalibog, Andre |
author_facet | Grodzik, Marta Szczepaniak, Jaroslaw Strojny-Cieslak, Barbara Hotowy, Anna Wierzbicki, Mateusz Jaworski, Slawomir Kutwin, Marta Soltan, Emilia Mandat, Tomasz Lewicka, Aneta Chwalibog, Andre |
author_sort | Grodzik, Marta |
collection | PubMed |
description | Our previous studies have shown that diamond nanoparticles (NDs) exhibited antiangiogenic and proapoptotic properties in vitro in glioblastoma multiforme (GBM) cells and in tumors in vivo. Moreover, NDs inhibited adhesion, leading to the suppression of migration and invasion of GBM. In the present study, we hypothesized that the NDs might also inhibit proliferation and cell cycle in glioma cells. Experiments were performed in vitro with the U87 and U118 lines of GBM cells, and for comparison, the Hs5 line of stromal cells (normal cells) after 24 h and 72 h of treatment. The analyses included cell morphology, cell death, viability, and cell cycle analysis, double timing assay, and gene expression (Rb, E2F1, CycA, CycB, CycD, CycE, PTEN, Ki-67). After 72 h of ND treatment, the expression level of Rb, CycD, and CycE in the U118 cells, and E2F1, CycD, and CycE in the U87 cells were significantly lower in comparison to those in the control group. We observed that decreased expression of cyclins inhibited the G1/S phase transition, arresting the cell cycle in the G0/G1 phase in glioma cells. The NDs did not affect the cell cycle as well as PTEN and Ki-67 expression in normal cells (Hs5), although it can be assumed that the NDs reduced proliferation and altered the cell cycle in fast dividing cells. |
format | Online Article Text |
id | pubmed-6515518 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65155182019-05-30 Diamond Nanoparticles Downregulate Expression of CycD and CycE in Glioma Cells Grodzik, Marta Szczepaniak, Jaroslaw Strojny-Cieslak, Barbara Hotowy, Anna Wierzbicki, Mateusz Jaworski, Slawomir Kutwin, Marta Soltan, Emilia Mandat, Tomasz Lewicka, Aneta Chwalibog, Andre Molecules Article Our previous studies have shown that diamond nanoparticles (NDs) exhibited antiangiogenic and proapoptotic properties in vitro in glioblastoma multiforme (GBM) cells and in tumors in vivo. Moreover, NDs inhibited adhesion, leading to the suppression of migration and invasion of GBM. In the present study, we hypothesized that the NDs might also inhibit proliferation and cell cycle in glioma cells. Experiments were performed in vitro with the U87 and U118 lines of GBM cells, and for comparison, the Hs5 line of stromal cells (normal cells) after 24 h and 72 h of treatment. The analyses included cell morphology, cell death, viability, and cell cycle analysis, double timing assay, and gene expression (Rb, E2F1, CycA, CycB, CycD, CycE, PTEN, Ki-67). After 72 h of ND treatment, the expression level of Rb, CycD, and CycE in the U118 cells, and E2F1, CycD, and CycE in the U87 cells were significantly lower in comparison to those in the control group. We observed that decreased expression of cyclins inhibited the G1/S phase transition, arresting the cell cycle in the G0/G1 phase in glioma cells. The NDs did not affect the cell cycle as well as PTEN and Ki-67 expression in normal cells (Hs5), although it can be assumed that the NDs reduced proliferation and altered the cell cycle in fast dividing cells. MDPI 2019-04-19 /pmc/articles/PMC6515518/ /pubmed/31010146 http://dx.doi.org/10.3390/molecules24081549 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Grodzik, Marta Szczepaniak, Jaroslaw Strojny-Cieslak, Barbara Hotowy, Anna Wierzbicki, Mateusz Jaworski, Slawomir Kutwin, Marta Soltan, Emilia Mandat, Tomasz Lewicka, Aneta Chwalibog, Andre Diamond Nanoparticles Downregulate Expression of CycD and CycE in Glioma Cells |
title | Diamond Nanoparticles Downregulate Expression of CycD and CycE in Glioma Cells |
title_full | Diamond Nanoparticles Downregulate Expression of CycD and CycE in Glioma Cells |
title_fullStr | Diamond Nanoparticles Downregulate Expression of CycD and CycE in Glioma Cells |
title_full_unstemmed | Diamond Nanoparticles Downregulate Expression of CycD and CycE in Glioma Cells |
title_short | Diamond Nanoparticles Downregulate Expression of CycD and CycE in Glioma Cells |
title_sort | diamond nanoparticles downregulate expression of cycd and cyce in glioma cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6515518/ https://www.ncbi.nlm.nih.gov/pubmed/31010146 http://dx.doi.org/10.3390/molecules24081549 |
work_keys_str_mv | AT grodzikmarta diamondnanoparticlesdownregulateexpressionofcycdandcyceingliomacells AT szczepaniakjaroslaw diamondnanoparticlesdownregulateexpressionofcycdandcyceingliomacells AT strojnycieslakbarbara diamondnanoparticlesdownregulateexpressionofcycdandcyceingliomacells AT hotowyanna diamondnanoparticlesdownregulateexpressionofcycdandcyceingliomacells AT wierzbickimateusz diamondnanoparticlesdownregulateexpressionofcycdandcyceingliomacells AT jaworskislawomir diamondnanoparticlesdownregulateexpressionofcycdandcyceingliomacells AT kutwinmarta diamondnanoparticlesdownregulateexpressionofcycdandcyceingliomacells AT soltanemilia diamondnanoparticlesdownregulateexpressionofcycdandcyceingliomacells AT mandattomasz diamondnanoparticlesdownregulateexpressionofcycdandcyceingliomacells AT lewickaaneta diamondnanoparticlesdownregulateexpressionofcycdandcyceingliomacells AT chwalibogandre diamondnanoparticlesdownregulateexpressionofcycdandcyceingliomacells |