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Diamond Nanoparticles Downregulate Expression of CycD and CycE in Glioma Cells

Our previous studies have shown that diamond nanoparticles (NDs) exhibited antiangiogenic and proapoptotic properties in vitro in glioblastoma multiforme (GBM) cells and in tumors in vivo. Moreover, NDs inhibited adhesion, leading to the suppression of migration and invasion of GBM. In the present s...

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Autores principales: Grodzik, Marta, Szczepaniak, Jaroslaw, Strojny-Cieslak, Barbara, Hotowy, Anna, Wierzbicki, Mateusz, Jaworski, Slawomir, Kutwin, Marta, Soltan, Emilia, Mandat, Tomasz, Lewicka, Aneta, Chwalibog, Andre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6515518/
https://www.ncbi.nlm.nih.gov/pubmed/31010146
http://dx.doi.org/10.3390/molecules24081549
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author Grodzik, Marta
Szczepaniak, Jaroslaw
Strojny-Cieslak, Barbara
Hotowy, Anna
Wierzbicki, Mateusz
Jaworski, Slawomir
Kutwin, Marta
Soltan, Emilia
Mandat, Tomasz
Lewicka, Aneta
Chwalibog, Andre
author_facet Grodzik, Marta
Szczepaniak, Jaroslaw
Strojny-Cieslak, Barbara
Hotowy, Anna
Wierzbicki, Mateusz
Jaworski, Slawomir
Kutwin, Marta
Soltan, Emilia
Mandat, Tomasz
Lewicka, Aneta
Chwalibog, Andre
author_sort Grodzik, Marta
collection PubMed
description Our previous studies have shown that diamond nanoparticles (NDs) exhibited antiangiogenic and proapoptotic properties in vitro in glioblastoma multiforme (GBM) cells and in tumors in vivo. Moreover, NDs inhibited adhesion, leading to the suppression of migration and invasion of GBM. In the present study, we hypothesized that the NDs might also inhibit proliferation and cell cycle in glioma cells. Experiments were performed in vitro with the U87 and U118 lines of GBM cells, and for comparison, the Hs5 line of stromal cells (normal cells) after 24 h and 72 h of treatment. The analyses included cell morphology, cell death, viability, and cell cycle analysis, double timing assay, and gene expression (Rb, E2F1, CycA, CycB, CycD, CycE, PTEN, Ki-67). After 72 h of ND treatment, the expression level of Rb, CycD, and CycE in the U118 cells, and E2F1, CycD, and CycE in the U87 cells were significantly lower in comparison to those in the control group. We observed that decreased expression of cyclins inhibited the G1/S phase transition, arresting the cell cycle in the G0/G1 phase in glioma cells. The NDs did not affect the cell cycle as well as PTEN and Ki-67 expression in normal cells (Hs5), although it can be assumed that the NDs reduced proliferation and altered the cell cycle in fast dividing cells.
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spelling pubmed-65155182019-05-30 Diamond Nanoparticles Downregulate Expression of CycD and CycE in Glioma Cells Grodzik, Marta Szczepaniak, Jaroslaw Strojny-Cieslak, Barbara Hotowy, Anna Wierzbicki, Mateusz Jaworski, Slawomir Kutwin, Marta Soltan, Emilia Mandat, Tomasz Lewicka, Aneta Chwalibog, Andre Molecules Article Our previous studies have shown that diamond nanoparticles (NDs) exhibited antiangiogenic and proapoptotic properties in vitro in glioblastoma multiforme (GBM) cells and in tumors in vivo. Moreover, NDs inhibited adhesion, leading to the suppression of migration and invasion of GBM. In the present study, we hypothesized that the NDs might also inhibit proliferation and cell cycle in glioma cells. Experiments were performed in vitro with the U87 and U118 lines of GBM cells, and for comparison, the Hs5 line of stromal cells (normal cells) after 24 h and 72 h of treatment. The analyses included cell morphology, cell death, viability, and cell cycle analysis, double timing assay, and gene expression (Rb, E2F1, CycA, CycB, CycD, CycE, PTEN, Ki-67). After 72 h of ND treatment, the expression level of Rb, CycD, and CycE in the U118 cells, and E2F1, CycD, and CycE in the U87 cells were significantly lower in comparison to those in the control group. We observed that decreased expression of cyclins inhibited the G1/S phase transition, arresting the cell cycle in the G0/G1 phase in glioma cells. The NDs did not affect the cell cycle as well as PTEN and Ki-67 expression in normal cells (Hs5), although it can be assumed that the NDs reduced proliferation and altered the cell cycle in fast dividing cells. MDPI 2019-04-19 /pmc/articles/PMC6515518/ /pubmed/31010146 http://dx.doi.org/10.3390/molecules24081549 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grodzik, Marta
Szczepaniak, Jaroslaw
Strojny-Cieslak, Barbara
Hotowy, Anna
Wierzbicki, Mateusz
Jaworski, Slawomir
Kutwin, Marta
Soltan, Emilia
Mandat, Tomasz
Lewicka, Aneta
Chwalibog, Andre
Diamond Nanoparticles Downregulate Expression of CycD and CycE in Glioma Cells
title Diamond Nanoparticles Downregulate Expression of CycD and CycE in Glioma Cells
title_full Diamond Nanoparticles Downregulate Expression of CycD and CycE in Glioma Cells
title_fullStr Diamond Nanoparticles Downregulate Expression of CycD and CycE in Glioma Cells
title_full_unstemmed Diamond Nanoparticles Downregulate Expression of CycD and CycE in Glioma Cells
title_short Diamond Nanoparticles Downregulate Expression of CycD and CycE in Glioma Cells
title_sort diamond nanoparticles downregulate expression of cycd and cyce in glioma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6515518/
https://www.ncbi.nlm.nih.gov/pubmed/31010146
http://dx.doi.org/10.3390/molecules24081549
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