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Comparison of the efficiency of different cell lysis methods and different commercial methods for RNA extraction from Candida albicans stored in RNAlater

BACKGROUND: Obtaining sufficient RNA yield and quality for comprehensive transcriptomic studies is cumbersome for clinical samples in which RNA from the pathogen is present in low numbers relative to the nucleic acids from the host, especially for pathogens, such as yeasts, with a solid cell wall. T...

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Detalles Bibliográficos
Autores principales: Rodríguez, Antonio, Vaneechoutte, Mario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6515685/
https://www.ncbi.nlm.nih.gov/pubmed/31088364
http://dx.doi.org/10.1186/s12866-019-1473-z