Seed mineral reserves and vigour of Bambara groundnut (Vigna subterranea L.) landraces differing in seed coat colour

A newly emerged seedling, given light and water, but no external source of minerals, uses its internal mineral nutrient reserves effectively for an early establishment. This research sought to investigate the influence of seed coat colour on the abundance of mineral elements in Bambara groundnut. Four landraces (G340A, Kazai, Kazuma, and Mana) varying in seed coat colour were analysed for differences in seed mineral composition using energy dispersive x-ray (EDX) analysis and atomic absorption spectrometry (AAS). Seeds were germinated at 10 °C and 25 °C, and various indices including, (1) mean emergence time, (2) mean germination rate, (3) coefficient of velocity of germination, and (4) final germination percentage (FGP) were calculated. The importance of seed mineral elements in the establishment of Bambara groundnut was examined by measuring root length (RL), shoot length (SL), shoot dry mass (SDM), and root dry mass (RDM). Plant tissue elemental analysis was done using flame atomic emission spectrometry (FAES) for K and flame atomic absorption spectrometry (FAAS) for Mg, Cu, Mn, and Zn. There were significant differences (P < 0.001) in mineral element content of dry seeds. G340A and Kazai had the highest and the lowest K, P, Mg, Mn, and Zn (11.65 gkg(-1), 7.2 gkg(-1), 2.33 gkg(-1), 59.56 mgkg(-1), and 44.42 mgkg(-1)), and (8.82 gkg(-1), 4.75 gkg(-1), 1.38 gkg(-1), 48.9 mgkg(-1), and 42.6 mgkg(-1)), respectively. Cold test germination indices were significantly different, the highest FGP was 73.3% in G340A and the lowest was 57.8% in Kazai. There were strong positive correlations between seed mineral concentration and plant growth parameters (p < 0.001). We concluded that (1) seed mineral concentration has a significant impact on the early establishment of Bambara groundnut and (2) the dark-coloured landraces (hue 8º) used in this study have the highest concentration of macro and micro elements compared to light coloured seeds (hue 38º).