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Combined Signature of the Fecal Microbiome and Plasma Metabolome in Patients with Ulcerative Colitis
BACKGROUND: Ulcerative colitis is a chronic, idiopathic inflammatory disease that destroys the colon structure. Nevertheless, the exact pathogenesis is not clear and needs to be fully elucidated. MATERIAL/METHODS: Stool and plasma samples were used for 16S ribosomal RNA sequencing and liquid chromat...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6515979/ https://www.ncbi.nlm.nih.gov/pubmed/31055592 http://dx.doi.org/10.12659/MSM.916009 |
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author | Sun, Meiling Du, Bing Shi, Yang Lu, Yue Zhou, Yangyang Liu, Bingrong |
author_facet | Sun, Meiling Du, Bing Shi, Yang Lu, Yue Zhou, Yangyang Liu, Bingrong |
author_sort | Sun, Meiling |
collection | PubMed |
description | BACKGROUND: Ulcerative colitis is a chronic, idiopathic inflammatory disease that destroys the colon structure. Nevertheless, the exact pathogenesis is not clear and needs to be fully elucidated. MATERIAL/METHODS: Stool and plasma samples were used for 16S ribosomal RNA sequencing and liquid chromatography mass spectrometry, respectively. In addition, we detected the level of trimethylamine N-oxide. Finally, we performed Pearson correlation analysis between the microbiome and the metabolome. RESULTS: Twenty-three active ulcerative colitis, 25 inactive ulcerative colitis, and 30 control cases were included. Thirty-four significantly different metabolites were found between the active ulcerative colitis and control groups, 38 were found between the inactive ulcerative colitis and control groups, and only 1 was found between the active ulcerative colitis and inactive ulcerative colitis groups. The plasma trimethylamine N-oxide level of the inactive ulcerative colitis and active ulcerative colitis groups was significantly higher than that of the control group. Moreover, we identified significant changes in 24, 18, and 12 bacterial genera for active ulcerative colitis-control, inactive ulcerative colitis-control, and active ulcerative colitis-inactive ulcerative colitis, respectively. Cross-correlation indicated an association between sphingosine 1-phosphate and Roseburia, Klebsiella, and Escherichia-Shigella. Through the pathway analysis, we found sphingolipid metabolism was one of the most significantly increased pathways. CONCLUSIONS: Although levels of trimethylamine N-oxide were higher in ulcerative colitis patients, they did not achieve statistical significance in active ulcerative colitis and inactive ulcerative colitis groups. Sphingosine 1-phosphate was increased in ulcerative colitis patients and there were several microbiota associated with it. Although further study is still needed, sphingosine 1-phosphate will probably become a new target for treatment of ulcerative colitis. |
format | Online Article Text |
id | pubmed-6515979 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-65159792019-07-29 Combined Signature of the Fecal Microbiome and Plasma Metabolome in Patients with Ulcerative Colitis Sun, Meiling Du, Bing Shi, Yang Lu, Yue Zhou, Yangyang Liu, Bingrong Med Sci Monit Clinical Research BACKGROUND: Ulcerative colitis is a chronic, idiopathic inflammatory disease that destroys the colon structure. Nevertheless, the exact pathogenesis is not clear and needs to be fully elucidated. MATERIAL/METHODS: Stool and plasma samples were used for 16S ribosomal RNA sequencing and liquid chromatography mass spectrometry, respectively. In addition, we detected the level of trimethylamine N-oxide. Finally, we performed Pearson correlation analysis between the microbiome and the metabolome. RESULTS: Twenty-three active ulcerative colitis, 25 inactive ulcerative colitis, and 30 control cases were included. Thirty-four significantly different metabolites were found between the active ulcerative colitis and control groups, 38 were found between the inactive ulcerative colitis and control groups, and only 1 was found between the active ulcerative colitis and inactive ulcerative colitis groups. The plasma trimethylamine N-oxide level of the inactive ulcerative colitis and active ulcerative colitis groups was significantly higher than that of the control group. Moreover, we identified significant changes in 24, 18, and 12 bacterial genera for active ulcerative colitis-control, inactive ulcerative colitis-control, and active ulcerative colitis-inactive ulcerative colitis, respectively. Cross-correlation indicated an association between sphingosine 1-phosphate and Roseburia, Klebsiella, and Escherichia-Shigella. Through the pathway analysis, we found sphingolipid metabolism was one of the most significantly increased pathways. CONCLUSIONS: Although levels of trimethylamine N-oxide were higher in ulcerative colitis patients, they did not achieve statistical significance in active ulcerative colitis and inactive ulcerative colitis groups. Sphingosine 1-phosphate was increased in ulcerative colitis patients and there were several microbiota associated with it. Although further study is still needed, sphingosine 1-phosphate will probably become a new target for treatment of ulcerative colitis. International Scientific Literature, Inc. 2019-05-05 /pmc/articles/PMC6515979/ /pubmed/31055592 http://dx.doi.org/10.12659/MSM.916009 Text en © Med Sci Monit, 2019 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) ) |
spellingShingle | Clinical Research Sun, Meiling Du, Bing Shi, Yang Lu, Yue Zhou, Yangyang Liu, Bingrong Combined Signature of the Fecal Microbiome and Plasma Metabolome in Patients with Ulcerative Colitis |
title | Combined Signature of the Fecal Microbiome and Plasma Metabolome in Patients with Ulcerative Colitis |
title_full | Combined Signature of the Fecal Microbiome and Plasma Metabolome in Patients with Ulcerative Colitis |
title_fullStr | Combined Signature of the Fecal Microbiome and Plasma Metabolome in Patients with Ulcerative Colitis |
title_full_unstemmed | Combined Signature of the Fecal Microbiome and Plasma Metabolome in Patients with Ulcerative Colitis |
title_short | Combined Signature of the Fecal Microbiome and Plasma Metabolome in Patients with Ulcerative Colitis |
title_sort | combined signature of the fecal microbiome and plasma metabolome in patients with ulcerative colitis |
topic | Clinical Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6515979/ https://www.ncbi.nlm.nih.gov/pubmed/31055592 http://dx.doi.org/10.12659/MSM.916009 |
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