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Metabolic profiling of zebrafish embryo development from blastula period to early larval stages

The zebrafish embryo is a popular model for drug screening, disease modelling and molecular genetics. In this study, samples were obtained from zebrafish at different developmental stages. The stages that were chosen were 3/4, 4/5, 24, 48, 72 and 96 hours post fertilization (hpf). Each sample includ...

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Autores principales: Dhillon, Sundeep S., Torell, Frida, Donten, Magdalena, Lundstedt-Enkel, Katrin, Bennett, Kate, Rännar, Stefan, Trygg, Johan, Lundstedt, Torbjörn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6516655/
https://www.ncbi.nlm.nih.gov/pubmed/31086370
http://dx.doi.org/10.1371/journal.pone.0213661
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author Dhillon, Sundeep S.
Torell, Frida
Donten, Magdalena
Lundstedt-Enkel, Katrin
Bennett, Kate
Rännar, Stefan
Trygg, Johan
Lundstedt, Torbjörn
author_facet Dhillon, Sundeep S.
Torell, Frida
Donten, Magdalena
Lundstedt-Enkel, Katrin
Bennett, Kate
Rännar, Stefan
Trygg, Johan
Lundstedt, Torbjörn
author_sort Dhillon, Sundeep S.
collection PubMed
description The zebrafish embryo is a popular model for drug screening, disease modelling and molecular genetics. In this study, samples were obtained from zebrafish at different developmental stages. The stages that were chosen were 3/4, 4/5, 24, 48, 72 and 96 hours post fertilization (hpf). Each sample included fifty embryos. The samples were analysed using gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). Principle component analysis (PCA) was applied to get an overview of the data and orthogonal projection to latent structure discriminant analysis (OPLS-DA) was utilised to discriminate between the developmental stages. In this way, changes in metabolite profiles during vertebrate development could be identified. Using a GC-TOF-MS metabolomics approach it was found that nucleotides and metabolic fuel (glucose) were elevated at early stages of embryogenesis, whereas at later stages amino acids and intermediates in the Krebs cycle were abundant. This agrees with zebrafish developmental biology, as organs such as the liver and pancreas develop at later stages. Thus, metabolomics of zebrafish embryos offers a unique opportunity to investigate large scale changes in metabolic processes during important developmental stages in vertebrate development. In terms of stability of the metabolic profile and viability of the embryos, it was concluded at 72 hpf was a suitable time point for the use of zebrafish as a model system in numerous scientific applications.
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spelling pubmed-65166552019-05-31 Metabolic profiling of zebrafish embryo development from blastula period to early larval stages Dhillon, Sundeep S. Torell, Frida Donten, Magdalena Lundstedt-Enkel, Katrin Bennett, Kate Rännar, Stefan Trygg, Johan Lundstedt, Torbjörn PLoS One Research Article The zebrafish embryo is a popular model for drug screening, disease modelling and molecular genetics. In this study, samples were obtained from zebrafish at different developmental stages. The stages that were chosen were 3/4, 4/5, 24, 48, 72 and 96 hours post fertilization (hpf). Each sample included fifty embryos. The samples were analysed using gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). Principle component analysis (PCA) was applied to get an overview of the data and orthogonal projection to latent structure discriminant analysis (OPLS-DA) was utilised to discriminate between the developmental stages. In this way, changes in metabolite profiles during vertebrate development could be identified. Using a GC-TOF-MS metabolomics approach it was found that nucleotides and metabolic fuel (glucose) were elevated at early stages of embryogenesis, whereas at later stages amino acids and intermediates in the Krebs cycle were abundant. This agrees with zebrafish developmental biology, as organs such as the liver and pancreas develop at later stages. Thus, metabolomics of zebrafish embryos offers a unique opportunity to investigate large scale changes in metabolic processes during important developmental stages in vertebrate development. In terms of stability of the metabolic profile and viability of the embryos, it was concluded at 72 hpf was a suitable time point for the use of zebrafish as a model system in numerous scientific applications. Public Library of Science 2019-05-14 /pmc/articles/PMC6516655/ /pubmed/31086370 http://dx.doi.org/10.1371/journal.pone.0213661 Text en © 2019 Dhillon et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Dhillon, Sundeep S.
Torell, Frida
Donten, Magdalena
Lundstedt-Enkel, Katrin
Bennett, Kate
Rännar, Stefan
Trygg, Johan
Lundstedt, Torbjörn
Metabolic profiling of zebrafish embryo development from blastula period to early larval stages
title Metabolic profiling of zebrafish embryo development from blastula period to early larval stages
title_full Metabolic profiling of zebrafish embryo development from blastula period to early larval stages
title_fullStr Metabolic profiling of zebrafish embryo development from blastula period to early larval stages
title_full_unstemmed Metabolic profiling of zebrafish embryo development from blastula period to early larval stages
title_short Metabolic profiling of zebrafish embryo development from blastula period to early larval stages
title_sort metabolic profiling of zebrafish embryo development from blastula period to early larval stages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6516655/
https://www.ncbi.nlm.nih.gov/pubmed/31086370
http://dx.doi.org/10.1371/journal.pone.0213661
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