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Beneficial Effect of Bariatric Surgery on Abnormal MMP-9 and AMPK Activities: Potential Markers of Obesity-Related CV Risk

Bariatric surgery (BS) results in sustained weight loss and may reverse inflammation, metabolic alterations, extracellular matrix remodeling and arterial stiffness. We hypothesize that increased stiffening in omental arteries from obese patients might be associated with an increase in MMP activity a...

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Autores principales: García-Prieto, Concha F., Gil-Ortega, Marta, Vega-Martín, Elena, Ramiro-Cortijo, David, Martín-Ramos, Miriam, Bordiú, Elena, Sanchez-Pernaute, Andrés, Torres, Antonio, Aránguez, I., Fernández-Alfonso, María, Rubio, Miguel A., Somoza, Beatriz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517546/
https://www.ncbi.nlm.nih.gov/pubmed/31133882
http://dx.doi.org/10.3389/fphys.2019.00553
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author García-Prieto, Concha F.
Gil-Ortega, Marta
Vega-Martín, Elena
Ramiro-Cortijo, David
Martín-Ramos, Miriam
Bordiú, Elena
Sanchez-Pernaute, Andrés
Torres, Antonio
Aránguez, I.
Fernández-Alfonso, María
Rubio, Miguel A.
Somoza, Beatriz
author_facet García-Prieto, Concha F.
Gil-Ortega, Marta
Vega-Martín, Elena
Ramiro-Cortijo, David
Martín-Ramos, Miriam
Bordiú, Elena
Sanchez-Pernaute, Andrés
Torres, Antonio
Aránguez, I.
Fernández-Alfonso, María
Rubio, Miguel A.
Somoza, Beatriz
author_sort García-Prieto, Concha F.
collection PubMed
description Bariatric surgery (BS) results in sustained weight loss and may reverse inflammation, metabolic alterations, extracellular matrix remodeling and arterial stiffness. We hypothesize that increased stiffening in omental arteries from obese patients might be associated with an increase in MMP activity and a decrease in p-AMPK, together with systemic oxidative stress and inflammation. Moreover, BS could contribute to reversing these alterations. This study was conducted with 38 patients of Caucasian origin: 31 adult patients with morbid obesity (9 men and 22 women; mean age 46 years and BMI = 42.7 ± 1.0 kg/m(2)) and 7 non-obese subjects (7 women; mean age 45 years and BMI = 22.7 ± 0.6 kg/m(2)). Seventeen obese patients were studied before and 12 months after BS. The stiffness index β, an index of intrinsic arterial stiffness, was determined in omental arteries and was significantly higher in obese patients. Levels of phosphorylated AMPK (p-AMPK(Thr-172)) and SIRT-1 were significantly lower in peripheral blood mononuclear cells (PBMCs) from obese patients than those from non-obese patients (p < 0.05) and were normalized after BS. Total and active MMP-9 activities, LDH, protein carbonyls and uric acid were higher in obese patients and reduced by BS. Moreover, there was a correlation between plasmatic LDH levels and the stiffness index β. BS has a beneficial effect on abnormal MMP-9, LDH and AMPK activities that might be associated with the development of arterial stiffness in obese patients. Since these parameters are easily measured in blood samples, they could constitute potential biomarkers of cardiovascular risk in morbid obesity.
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spelling pubmed-65175462019-05-27 Beneficial Effect of Bariatric Surgery on Abnormal MMP-9 and AMPK Activities: Potential Markers of Obesity-Related CV Risk García-Prieto, Concha F. Gil-Ortega, Marta Vega-Martín, Elena Ramiro-Cortijo, David Martín-Ramos, Miriam Bordiú, Elena Sanchez-Pernaute, Andrés Torres, Antonio Aránguez, I. Fernández-Alfonso, María Rubio, Miguel A. Somoza, Beatriz Front Physiol Physiology Bariatric surgery (BS) results in sustained weight loss and may reverse inflammation, metabolic alterations, extracellular matrix remodeling and arterial stiffness. We hypothesize that increased stiffening in omental arteries from obese patients might be associated with an increase in MMP activity and a decrease in p-AMPK, together with systemic oxidative stress and inflammation. Moreover, BS could contribute to reversing these alterations. This study was conducted with 38 patients of Caucasian origin: 31 adult patients with morbid obesity (9 men and 22 women; mean age 46 years and BMI = 42.7 ± 1.0 kg/m(2)) and 7 non-obese subjects (7 women; mean age 45 years and BMI = 22.7 ± 0.6 kg/m(2)). Seventeen obese patients were studied before and 12 months after BS. The stiffness index β, an index of intrinsic arterial stiffness, was determined in omental arteries and was significantly higher in obese patients. Levels of phosphorylated AMPK (p-AMPK(Thr-172)) and SIRT-1 were significantly lower in peripheral blood mononuclear cells (PBMCs) from obese patients than those from non-obese patients (p < 0.05) and were normalized after BS. Total and active MMP-9 activities, LDH, protein carbonyls and uric acid were higher in obese patients and reduced by BS. Moreover, there was a correlation between plasmatic LDH levels and the stiffness index β. BS has a beneficial effect on abnormal MMP-9, LDH and AMPK activities that might be associated with the development of arterial stiffness in obese patients. Since these parameters are easily measured in blood samples, they could constitute potential biomarkers of cardiovascular risk in morbid obesity. Frontiers Media S.A. 2019-05-08 /pmc/articles/PMC6517546/ /pubmed/31133882 http://dx.doi.org/10.3389/fphys.2019.00553 Text en Copyright © 2019 García-Prieto, Gil-Ortega, Vega-Martín, Ramiro-Cortijo, Martín-Ramos, Bordiú, Sanchez-Pernaute, Torres, Aránguez, Fernández-Alfonso, Rubio and Somoza. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
García-Prieto, Concha F.
Gil-Ortega, Marta
Vega-Martín, Elena
Ramiro-Cortijo, David
Martín-Ramos, Miriam
Bordiú, Elena
Sanchez-Pernaute, Andrés
Torres, Antonio
Aránguez, I.
Fernández-Alfonso, María
Rubio, Miguel A.
Somoza, Beatriz
Beneficial Effect of Bariatric Surgery on Abnormal MMP-9 and AMPK Activities: Potential Markers of Obesity-Related CV Risk
title Beneficial Effect of Bariatric Surgery on Abnormal MMP-9 and AMPK Activities: Potential Markers of Obesity-Related CV Risk
title_full Beneficial Effect of Bariatric Surgery on Abnormal MMP-9 and AMPK Activities: Potential Markers of Obesity-Related CV Risk
title_fullStr Beneficial Effect of Bariatric Surgery on Abnormal MMP-9 and AMPK Activities: Potential Markers of Obesity-Related CV Risk
title_full_unstemmed Beneficial Effect of Bariatric Surgery on Abnormal MMP-9 and AMPK Activities: Potential Markers of Obesity-Related CV Risk
title_short Beneficial Effect of Bariatric Surgery on Abnormal MMP-9 and AMPK Activities: Potential Markers of Obesity-Related CV Risk
title_sort beneficial effect of bariatric surgery on abnormal mmp-9 and ampk activities: potential markers of obesity-related cv risk
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517546/
https://www.ncbi.nlm.nih.gov/pubmed/31133882
http://dx.doi.org/10.3389/fphys.2019.00553
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