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Identifying the Growth Modulon of Corynebacterium glutamicum

The growth rate (μ) of industrially relevant microbes, such as Corynebacterium glutamicum, is a fundamental property that indicates its production capacity. Therefore, understanding the mechanism underlying the growth rate is imperative for improving productivity and performance through metabolic en...

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Autores principales: Haas, Thorsten, Graf, Michaela, Nieß, Alexander, Busche, Tobias, Kalinowski, Jörn, Blombach, Bastian, Takors, Ralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517550/
https://www.ncbi.nlm.nih.gov/pubmed/31134020
http://dx.doi.org/10.3389/fmicb.2019.00974
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author Haas, Thorsten
Graf, Michaela
Nieß, Alexander
Busche, Tobias
Kalinowski, Jörn
Blombach, Bastian
Takors, Ralf
author_facet Haas, Thorsten
Graf, Michaela
Nieß, Alexander
Busche, Tobias
Kalinowski, Jörn
Blombach, Bastian
Takors, Ralf
author_sort Haas, Thorsten
collection PubMed
description The growth rate (μ) of industrially relevant microbes, such as Corynebacterium glutamicum, is a fundamental property that indicates its production capacity. Therefore, understanding the mechanism underlying the growth rate is imperative for improving productivity and performance through metabolic engineering. Despite recent progress in the understanding of global regulatory interactions, knowledge of mechanisms directing cell growth remains fragmented and incomplete. The current study investigated RNA-Seq data of three growth rate transitions, induced by different pre-culture conditions, in order to identify transcriptomic changes corresponding to increasing growth rates. These transitions took place in minimal medium and ranged from 0.02 to 0.4 h(-1) μ. This study enabled the identification of 447 genes as components of the growth modulon. Enrichment of genes within the growth modulon revealed 10 regulons exhibiting a significant effect over growth rate transition. In summary, central metabolism was observed to be regulated by a combination of metabolic and transcriptional activities orchestrating control over glycolysis, pentose phosphate pathway, and the tricarboxylic acid cycle. Additionally, major responses to changes in the growth rate were linked to iron uptake and carbon metabolism. In particular, genes encoding glycolytic enzymes and the glucose uptake system showed a positive correlation with the growth rate.
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spelling pubmed-65175502019-05-27 Identifying the Growth Modulon of Corynebacterium glutamicum Haas, Thorsten Graf, Michaela Nieß, Alexander Busche, Tobias Kalinowski, Jörn Blombach, Bastian Takors, Ralf Front Microbiol Microbiology The growth rate (μ) of industrially relevant microbes, such as Corynebacterium glutamicum, is a fundamental property that indicates its production capacity. Therefore, understanding the mechanism underlying the growth rate is imperative for improving productivity and performance through metabolic engineering. Despite recent progress in the understanding of global regulatory interactions, knowledge of mechanisms directing cell growth remains fragmented and incomplete. The current study investigated RNA-Seq data of three growth rate transitions, induced by different pre-culture conditions, in order to identify transcriptomic changes corresponding to increasing growth rates. These transitions took place in minimal medium and ranged from 0.02 to 0.4 h(-1) μ. This study enabled the identification of 447 genes as components of the growth modulon. Enrichment of genes within the growth modulon revealed 10 regulons exhibiting a significant effect over growth rate transition. In summary, central metabolism was observed to be regulated by a combination of metabolic and transcriptional activities orchestrating control over glycolysis, pentose phosphate pathway, and the tricarboxylic acid cycle. Additionally, major responses to changes in the growth rate were linked to iron uptake and carbon metabolism. In particular, genes encoding glycolytic enzymes and the glucose uptake system showed a positive correlation with the growth rate. Frontiers Media S.A. 2019-05-08 /pmc/articles/PMC6517550/ /pubmed/31134020 http://dx.doi.org/10.3389/fmicb.2019.00974 Text en Copyright © 2019 Haas, Graf, Nieß, Busche, Kalinowski, Blombach and Takors. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Haas, Thorsten
Graf, Michaela
Nieß, Alexander
Busche, Tobias
Kalinowski, Jörn
Blombach, Bastian
Takors, Ralf
Identifying the Growth Modulon of Corynebacterium glutamicum
title Identifying the Growth Modulon of Corynebacterium glutamicum
title_full Identifying the Growth Modulon of Corynebacterium glutamicum
title_fullStr Identifying the Growth Modulon of Corynebacterium glutamicum
title_full_unstemmed Identifying the Growth Modulon of Corynebacterium glutamicum
title_short Identifying the Growth Modulon of Corynebacterium glutamicum
title_sort identifying the growth modulon of corynebacterium glutamicum
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517550/
https://www.ncbi.nlm.nih.gov/pubmed/31134020
http://dx.doi.org/10.3389/fmicb.2019.00974
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