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A de novo transcriptome assembly approach elucidates the dynamics of ovarian maturation in the swordfish (Xiphias gladius)

The Mediterranean swordfish (Xiphias gladius) has been recently classified as overfished and in 2016, the International Commission for the Conservation of the Atlantic Tunas (ICCAT) established a multi-annual management plan to recover this stock. To successfully achieve this goal, knowledge about s...

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Autores principales: Gioacchini, Giorgia, Marisaldi, Luca, Basili, Danilo, Candelma, Michela, Pignalosa, Paolo, Aiese Cigliano, Riccardo, Sanseverino, Walter, Hardiman, Gary, Carnevali, Oliana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517582/
https://www.ncbi.nlm.nih.gov/pubmed/31089194
http://dx.doi.org/10.1038/s41598-019-43872-6
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author Gioacchini, Giorgia
Marisaldi, Luca
Basili, Danilo
Candelma, Michela
Pignalosa, Paolo
Aiese Cigliano, Riccardo
Sanseverino, Walter
Hardiman, Gary
Carnevali, Oliana
author_facet Gioacchini, Giorgia
Marisaldi, Luca
Basili, Danilo
Candelma, Michela
Pignalosa, Paolo
Aiese Cigliano, Riccardo
Sanseverino, Walter
Hardiman, Gary
Carnevali, Oliana
author_sort Gioacchini, Giorgia
collection PubMed
description The Mediterranean swordfish (Xiphias gladius) has been recently classified as overfished and in 2016, the International Commission for the Conservation of the Atlantic Tunas (ICCAT) established a multi-annual management plan to recover this stock. To successfully achieve this goal, knowledge about swordfish biology is needed. To date, few studies on swordfish have been performed and none of them has provided useful insights into the reproductive biology at molecular level. Here we set to characterise the molecular dynamics underlying ovarian maturation by employing a de novo transcriptome assembly approach. Differential gene expression analysis in mature and immature ovaries identified a number of differentially expressed genes associated with biological processes driving ovarian maturation. Focusing on ovarian steroidogenesis and vitellogenin uptake, we depict the molecular dynamics characterizing these processes while a phylogenetic analysis let us identify a candidate vitellogenin receptor. This is the first swordfish transcriptome assembly and these findings provide in-depth understanding of molecular processes describing ovarian maturation. Moreover, the establishment of a publicly available database containing information on the swordfish transcriptome aims to boost research on this species with the long-term of developing more comprehensive and successful stock management plans.
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spelling pubmed-65175822019-05-24 A de novo transcriptome assembly approach elucidates the dynamics of ovarian maturation in the swordfish (Xiphias gladius) Gioacchini, Giorgia Marisaldi, Luca Basili, Danilo Candelma, Michela Pignalosa, Paolo Aiese Cigliano, Riccardo Sanseverino, Walter Hardiman, Gary Carnevali, Oliana Sci Rep Article The Mediterranean swordfish (Xiphias gladius) has been recently classified as overfished and in 2016, the International Commission for the Conservation of the Atlantic Tunas (ICCAT) established a multi-annual management plan to recover this stock. To successfully achieve this goal, knowledge about swordfish biology is needed. To date, few studies on swordfish have been performed and none of them has provided useful insights into the reproductive biology at molecular level. Here we set to characterise the molecular dynamics underlying ovarian maturation by employing a de novo transcriptome assembly approach. Differential gene expression analysis in mature and immature ovaries identified a number of differentially expressed genes associated with biological processes driving ovarian maturation. Focusing on ovarian steroidogenesis and vitellogenin uptake, we depict the molecular dynamics characterizing these processes while a phylogenetic analysis let us identify a candidate vitellogenin receptor. This is the first swordfish transcriptome assembly and these findings provide in-depth understanding of molecular processes describing ovarian maturation. Moreover, the establishment of a publicly available database containing information on the swordfish transcriptome aims to boost research on this species with the long-term of developing more comprehensive and successful stock management plans. Nature Publishing Group UK 2019-05-14 /pmc/articles/PMC6517582/ /pubmed/31089194 http://dx.doi.org/10.1038/s41598-019-43872-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Gioacchini, Giorgia
Marisaldi, Luca
Basili, Danilo
Candelma, Michela
Pignalosa, Paolo
Aiese Cigliano, Riccardo
Sanseverino, Walter
Hardiman, Gary
Carnevali, Oliana
A de novo transcriptome assembly approach elucidates the dynamics of ovarian maturation in the swordfish (Xiphias gladius)
title A de novo transcriptome assembly approach elucidates the dynamics of ovarian maturation in the swordfish (Xiphias gladius)
title_full A de novo transcriptome assembly approach elucidates the dynamics of ovarian maturation in the swordfish (Xiphias gladius)
title_fullStr A de novo transcriptome assembly approach elucidates the dynamics of ovarian maturation in the swordfish (Xiphias gladius)
title_full_unstemmed A de novo transcriptome assembly approach elucidates the dynamics of ovarian maturation in the swordfish (Xiphias gladius)
title_short A de novo transcriptome assembly approach elucidates the dynamics of ovarian maturation in the swordfish (Xiphias gladius)
title_sort de novo transcriptome assembly approach elucidates the dynamics of ovarian maturation in the swordfish (xiphias gladius)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517582/
https://www.ncbi.nlm.nih.gov/pubmed/31089194
http://dx.doi.org/10.1038/s41598-019-43872-6
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