Synthetic Design of Asymmetric miRNA with an Engineered 3′ Overhang to Improve Strand Selection

We developed a novel miRNA design that significantly improves strand selection within the RISC complex by engineering the 3′ end by adding extra nucleotides. Addition of seven nucleotides at the 3′ ends of the miR or miR* strand resulted in a thermodynamic asymmetry at either of the two ends, which...

Descripción completa

Detalles Bibliográficos
Autores principales: Kadekar, Sandeep, Nawale, Ganesh N., Karlsson, Kira, Ålander, Cecilia, Oommen, Oommen P., Varghese, Oommen P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517641/
https://www.ncbi.nlm.nih.gov/pubmed/31085353
http://dx.doi.org/10.1016/j.omtn.2019.04.012

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517641/
https://www.ncbi.nlm.nih.gov/pubmed/31085353
http://dx.doi.org/10.1016/j.omtn.2019.04.012

Ejemplares similares