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Synthetic Design of Asymmetric miRNA with an Engineered 3′ Overhang to Improve Strand Selection
We developed a novel miRNA design that significantly improves strand selection within the RISC complex by engineering the 3′ end by adding extra nucleotides. Addition of seven nucleotides at the 3′ ends of the miR or miR* strand resulted in a thermodynamic asymmetry at either of the two ends, which...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Society of Gene & Cell Therapy
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517641/ https://www.ncbi.nlm.nih.gov/pubmed/31085353 http://dx.doi.org/10.1016/j.omtn.2019.04.012 |