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HPV infection in semen: results from a new molecular approach

Human papillomavirus (HPV) is the agent of the most common sexually transmitted diseases causing a variety of clinical manifestations ranging from warts to cancer. Oncogenic HPV infection is the major cause of cervical cancer and less frequently of penile cancers. Its presence in semen is widely kno...

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Autores principales: Capra, G., Schillaci, R., Bosco, L., Roccheri, M. C., Perino, A., Ragusa, M. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cambridge University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6518472/
https://www.ncbi.nlm.nih.gov/pubmed/31063107
http://dx.doi.org/10.1017/S0950268819000621
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author Capra, G.
Schillaci, R.
Bosco, L.
Roccheri, M. C.
Perino, A.
Ragusa, M. A.
author_facet Capra, G.
Schillaci, R.
Bosco, L.
Roccheri, M. C.
Perino, A.
Ragusa, M. A.
author_sort Capra, G.
collection PubMed
description Human papillomavirus (HPV) is the agent of the most common sexually transmitted diseases causing a variety of clinical manifestations ranging from warts to cancer. Oncogenic HPV infection is the major cause of cervical cancer and less frequently of penile cancers. Its presence in semen is widely known, but the effects on fertility are still controversial. We developed a new approach to evaluate virus localisation in the different semen components. We analysed also the specific genotype localisation and viral DNA quantity by qPCR. Results show that HPV DNA can be identified in every fraction of semen: spermatozoa, somatic cells and seminal plasma. Different samples can contain the HPV DNA in different fractions and several HPV genotypes can be found in the same fraction. Additionally, different fractions may contain multiple HPV genotypes in different relative quantity. We analysed the wholeness of HPV DNA in sperm cells by qPCR. In one sample more than half of viral genomes were defective, suggesting a possible recombination event. The new method allows to easily distinguish different sperm infections and to observe the possible effects on semen. The data support the proposed role of HPV in decreased fertility and prompt new possible consequences of the infection in semen.
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spelling pubmed-65184722019-06-04 HPV infection in semen: results from a new molecular approach Capra, G. Schillaci, R. Bosco, L. Roccheri, M. C. Perino, A. Ragusa, M. A. Epidemiol Infect Original Paper Human papillomavirus (HPV) is the agent of the most common sexually transmitted diseases causing a variety of clinical manifestations ranging from warts to cancer. Oncogenic HPV infection is the major cause of cervical cancer and less frequently of penile cancers. Its presence in semen is widely known, but the effects on fertility are still controversial. We developed a new approach to evaluate virus localisation in the different semen components. We analysed also the specific genotype localisation and viral DNA quantity by qPCR. Results show that HPV DNA can be identified in every fraction of semen: spermatozoa, somatic cells and seminal plasma. Different samples can contain the HPV DNA in different fractions and several HPV genotypes can be found in the same fraction. Additionally, different fractions may contain multiple HPV genotypes in different relative quantity. We analysed the wholeness of HPV DNA in sperm cells by qPCR. In one sample more than half of viral genomes were defective, suggesting a possible recombination event. The new method allows to easily distinguish different sperm infections and to observe the possible effects on semen. The data support the proposed role of HPV in decreased fertility and prompt new possible consequences of the infection in semen. Cambridge University Press 2019-04-15 /pmc/articles/PMC6518472/ /pubmed/31063107 http://dx.doi.org/10.1017/S0950268819000621 Text en © The Author(s) 2019 http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is unaltered and is properly cited. The written permission of Cambridge University Press must be obtained for commercial re-use or in order to create a derivative work.
spellingShingle Original Paper
Capra, G.
Schillaci, R.
Bosco, L.
Roccheri, M. C.
Perino, A.
Ragusa, M. A.
HPV infection in semen: results from a new molecular approach
title HPV infection in semen: results from a new molecular approach
title_full HPV infection in semen: results from a new molecular approach
title_fullStr HPV infection in semen: results from a new molecular approach
title_full_unstemmed HPV infection in semen: results from a new molecular approach
title_short HPV infection in semen: results from a new molecular approach
title_sort hpv infection in semen: results from a new molecular approach
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6518472/
https://www.ncbi.nlm.nih.gov/pubmed/31063107
http://dx.doi.org/10.1017/S0950268819000621
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