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Variation in the AvrSr35 gene determines Sr35 resistance against wheat stem rust race Ug99
Puccinia graminis f. sp. tritici (Pgt) causes wheat stem rust, a devastating fungal disease. The Sr35 resistance gene confers immunity against this pathogen’s most virulent races, including Ug99. We used comparative whole-genome sequencing of chemically mutagenized and natural Pgt isolates to identi...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6518949/ https://www.ncbi.nlm.nih.gov/pubmed/29269474 http://dx.doi.org/10.1126/science.aao7294 |
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author | Salcedo, Andres Rutter, William Wang, Shichen Akhunova, Alina Bolus, Stephen Chao, Shiaoman Anderson, Nickolas De Soto, Monica Fernandez Rouse, Matthew Szabo, Les Bowden, Robert L. Dubcovsky, Jorge Akhunov, Eduard |
author_facet | Salcedo, Andres Rutter, William Wang, Shichen Akhunova, Alina Bolus, Stephen Chao, Shiaoman Anderson, Nickolas De Soto, Monica Fernandez Rouse, Matthew Szabo, Les Bowden, Robert L. Dubcovsky, Jorge Akhunov, Eduard |
author_sort | Salcedo, Andres |
collection | PubMed |
description | Puccinia graminis f. sp. tritici (Pgt) causes wheat stem rust, a devastating fungal disease. The Sr35 resistance gene confers immunity against this pathogen’s most virulent races, including Ug99. We used comparative whole-genome sequencing of chemically mutagenized and natural Pgt isolates to identify a fungal gene named AvrSr35 that is required for Sr35 avirulence. The AvrSr35 gene encodes a secreted protein capable of interacting with Sr35 and triggering the immune response. We show that the origin of Pgt isolates virulent on Sr35 is associated with the nonfunctionalization of the AvrSr35 gene by the insertion of a mobile element. The discovery of AvrSr35 provides a new tool for Pgt surveillance, identification of host susceptibility targets, and characterization of the molecular determinants of immunity in wheat. |
format | Online Article Text |
id | pubmed-6518949 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-65189492019-05-30 Variation in the AvrSr35 gene determines Sr35 resistance against wheat stem rust race Ug99 Salcedo, Andres Rutter, William Wang, Shichen Akhunova, Alina Bolus, Stephen Chao, Shiaoman Anderson, Nickolas De Soto, Monica Fernandez Rouse, Matthew Szabo, Les Bowden, Robert L. Dubcovsky, Jorge Akhunov, Eduard Science Plant Science Puccinia graminis f. sp. tritici (Pgt) causes wheat stem rust, a devastating fungal disease. The Sr35 resistance gene confers immunity against this pathogen’s most virulent races, including Ug99. We used comparative whole-genome sequencing of chemically mutagenized and natural Pgt isolates to identify a fungal gene named AvrSr35 that is required for Sr35 avirulence. The AvrSr35 gene encodes a secreted protein capable of interacting with Sr35 and triggering the immune response. We show that the origin of Pgt isolates virulent on Sr35 is associated with the nonfunctionalization of the AvrSr35 gene by the insertion of a mobile element. The discovery of AvrSr35 provides a new tool for Pgt surveillance, identification of host susceptibility targets, and characterization of the molecular determinants of immunity in wheat. American Association for the Advancement of Science 2017-12-22 2017 /pmc/articles/PMC6518949/ /pubmed/29269474 http://dx.doi.org/10.1126/science.aao7294 Text en 2017 © The Authors, some rights reserved http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Plant Science Salcedo, Andres Rutter, William Wang, Shichen Akhunova, Alina Bolus, Stephen Chao, Shiaoman Anderson, Nickolas De Soto, Monica Fernandez Rouse, Matthew Szabo, Les Bowden, Robert L. Dubcovsky, Jorge Akhunov, Eduard Variation in the AvrSr35 gene determines Sr35 resistance against wheat stem rust race Ug99 |
title | Variation in the AvrSr35 gene determines Sr35 resistance against wheat stem rust race Ug99 |
title_full | Variation in the AvrSr35 gene determines Sr35 resistance against wheat stem rust race Ug99 |
title_fullStr | Variation in the AvrSr35 gene determines Sr35 resistance against wheat stem rust race Ug99 |
title_full_unstemmed | Variation in the AvrSr35 gene determines Sr35 resistance against wheat stem rust race Ug99 |
title_short | Variation in the AvrSr35 gene determines Sr35 resistance against wheat stem rust race Ug99 |
title_sort | variation in the avrsr35 gene determines sr35 resistance against wheat stem rust race ug99 |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6518949/ https://www.ncbi.nlm.nih.gov/pubmed/29269474 http://dx.doi.org/10.1126/science.aao7294 |
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