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Cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: An in vitro study
BACKGROUND: Biosynthesized silver nanoparticles (AgNPs) have been proposed as effective antimicrobial agents against endo–perio pathogens. Determination of cytotoxicity is important for effective clinical use. AIM: The aim is to determine the cytotoxicity of fungal-derived AgNPs on human gingival fi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519193/ https://www.ncbi.nlm.nih.gov/pubmed/31142986 http://dx.doi.org/10.4103/JCD.JCD_518_18 |
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author | Halkai, Kiran R. Mudda, Jayashree A. Shivanna, Vasundhara Patil, Veena Rathod, Vandana Halkai, Rahul |
author_facet | Halkai, Kiran R. Mudda, Jayashree A. Shivanna, Vasundhara Patil, Veena Rathod, Vandana Halkai, Rahul |
author_sort | Halkai, Kiran R. |
collection | PubMed |
description | BACKGROUND: Biosynthesized silver nanoparticles (AgNPs) have been proposed as effective antimicrobial agents against endo–perio pathogens. Determination of cytotoxicity is important for effective clinical use. AIM: The aim is to determine the cytotoxicity of fungal-derived AgNPs on human gingival fibroblast (HGF) cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. MATERIALS AND METHODS: HGF cell cultures were trypsinized and adjusted to 5 × 10(3) cells/ml and 100-μl cell suspension (50,000 cells/well) and were added to 96-well plate. After 24 h, 100 μl of AgNPs (8–512-μg/ml concentrations) was added and incubated at 37°C for 24 h in 5% CO(2) atmosphere. Controls were used without AgNPs. MTT (1 mg/ml) was added and incubated for 4 h at 37°C in 5% CO(2) atmosphere. Microscopic examination was done, and absorbance was measured using a microplate reader at a wavelength of 540 nm. Percentage growth inhibition was calculated, and the concentration of AgNPs needed to inhibit cell growth by 50% (CTC(50)) was generated. RESULTS: CTC(50) was found at a concentration of 260 μg/ml. AgNPs exerted less cytotoxicity against HGF cell line and increased with increase in the concentration of AgNPs. CONCLUSION: Fungal-derived AgNPs are safe to healthy cells at a concentration <260 μg/ml. Therefore, they can be effectively used for the treatment of endo–perio lesions. |
format | Online Article Text |
id | pubmed-6519193 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-65191932019-05-29 Cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: An in vitro study Halkai, Kiran R. Mudda, Jayashree A. Shivanna, Vasundhara Patil, Veena Rathod, Vandana Halkai, Rahul J Conserv Dent Original Article BACKGROUND: Biosynthesized silver nanoparticles (AgNPs) have been proposed as effective antimicrobial agents against endo–perio pathogens. Determination of cytotoxicity is important for effective clinical use. AIM: The aim is to determine the cytotoxicity of fungal-derived AgNPs on human gingival fibroblast (HGF) cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. MATERIALS AND METHODS: HGF cell cultures were trypsinized and adjusted to 5 × 10(3) cells/ml and 100-μl cell suspension (50,000 cells/well) and were added to 96-well plate. After 24 h, 100 μl of AgNPs (8–512-μg/ml concentrations) was added and incubated at 37°C for 24 h in 5% CO(2) atmosphere. Controls were used without AgNPs. MTT (1 mg/ml) was added and incubated for 4 h at 37°C in 5% CO(2) atmosphere. Microscopic examination was done, and absorbance was measured using a microplate reader at a wavelength of 540 nm. Percentage growth inhibition was calculated, and the concentration of AgNPs needed to inhibit cell growth by 50% (CTC(50)) was generated. RESULTS: CTC(50) was found at a concentration of 260 μg/ml. AgNPs exerted less cytotoxicity against HGF cell line and increased with increase in the concentration of AgNPs. CONCLUSION: Fungal-derived AgNPs are safe to healthy cells at a concentration <260 μg/ml. Therefore, they can be effectively used for the treatment of endo–perio lesions. Medknow Publications & Media Pvt Ltd 2019 /pmc/articles/PMC6519193/ /pubmed/31142986 http://dx.doi.org/10.4103/JCD.JCD_518_18 Text en Copyright: © 2019 Journal of Conservative Dentistry http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Halkai, Kiran R. Mudda, Jayashree A. Shivanna, Vasundhara Patil, Veena Rathod, Vandana Halkai, Rahul Cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: An in vitro study |
title | Cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: An in vitro study |
title_full | Cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: An in vitro study |
title_fullStr | Cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: An in vitro study |
title_full_unstemmed | Cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: An in vitro study |
title_short | Cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: An in vitro study |
title_sort | cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: an in vitro study |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519193/ https://www.ncbi.nlm.nih.gov/pubmed/31142986 http://dx.doi.org/10.4103/JCD.JCD_518_18 |
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