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Cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: An in vitro study

BACKGROUND: Biosynthesized silver nanoparticles (AgNPs) have been proposed as effective antimicrobial agents against endo–perio pathogens. Determination of cytotoxicity is important for effective clinical use. AIM: The aim is to determine the cytotoxicity of fungal-derived AgNPs on human gingival fi...

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Autores principales: Halkai, Kiran R., Mudda, Jayashree A., Shivanna, Vasundhara, Patil, Veena, Rathod, Vandana, Halkai, Rahul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519193/
https://www.ncbi.nlm.nih.gov/pubmed/31142986
http://dx.doi.org/10.4103/JCD.JCD_518_18
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author Halkai, Kiran R.
Mudda, Jayashree A.
Shivanna, Vasundhara
Patil, Veena
Rathod, Vandana
Halkai, Rahul
author_facet Halkai, Kiran R.
Mudda, Jayashree A.
Shivanna, Vasundhara
Patil, Veena
Rathod, Vandana
Halkai, Rahul
author_sort Halkai, Kiran R.
collection PubMed
description BACKGROUND: Biosynthesized silver nanoparticles (AgNPs) have been proposed as effective antimicrobial agents against endo–perio pathogens. Determination of cytotoxicity is important for effective clinical use. AIM: The aim is to determine the cytotoxicity of fungal-derived AgNPs on human gingival fibroblast (HGF) cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. MATERIALS AND METHODS: HGF cell cultures were trypsinized and adjusted to 5 × 10(3) cells/ml and 100-μl cell suspension (50,000 cells/well) and were added to 96-well plate. After 24 h, 100 μl of AgNPs (8–512-μg/ml concentrations) was added and incubated at 37°C for 24 h in 5% CO(2) atmosphere. Controls were used without AgNPs. MTT (1 mg/ml) was added and incubated for 4 h at 37°C in 5% CO(2) atmosphere. Microscopic examination was done, and absorbance was measured using a microplate reader at a wavelength of 540 nm. Percentage growth inhibition was calculated, and the concentration of AgNPs needed to inhibit cell growth by 50% (CTC(50)) was generated. RESULTS: CTC(50) was found at a concentration of 260 μg/ml. AgNPs exerted less cytotoxicity against HGF cell line and increased with increase in the concentration of AgNPs. CONCLUSION: Fungal-derived AgNPs are safe to healthy cells at a concentration <260 μg/ml. Therefore, they can be effectively used for the treatment of endo–perio lesions.
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spelling pubmed-65191932019-05-29 Cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: An in vitro study Halkai, Kiran R. Mudda, Jayashree A. Shivanna, Vasundhara Patil, Veena Rathod, Vandana Halkai, Rahul J Conserv Dent Original Article BACKGROUND: Biosynthesized silver nanoparticles (AgNPs) have been proposed as effective antimicrobial agents against endo–perio pathogens. Determination of cytotoxicity is important for effective clinical use. AIM: The aim is to determine the cytotoxicity of fungal-derived AgNPs on human gingival fibroblast (HGF) cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. MATERIALS AND METHODS: HGF cell cultures were trypsinized and adjusted to 5 × 10(3) cells/ml and 100-μl cell suspension (50,000 cells/well) and were added to 96-well plate. After 24 h, 100 μl of AgNPs (8–512-μg/ml concentrations) was added and incubated at 37°C for 24 h in 5% CO(2) atmosphere. Controls were used without AgNPs. MTT (1 mg/ml) was added and incubated for 4 h at 37°C in 5% CO(2) atmosphere. Microscopic examination was done, and absorbance was measured using a microplate reader at a wavelength of 540 nm. Percentage growth inhibition was calculated, and the concentration of AgNPs needed to inhibit cell growth by 50% (CTC(50)) was generated. RESULTS: CTC(50) was found at a concentration of 260 μg/ml. AgNPs exerted less cytotoxicity against HGF cell line and increased with increase in the concentration of AgNPs. CONCLUSION: Fungal-derived AgNPs are safe to healthy cells at a concentration <260 μg/ml. Therefore, they can be effectively used for the treatment of endo–perio lesions. Medknow Publications & Media Pvt Ltd 2019 /pmc/articles/PMC6519193/ /pubmed/31142986 http://dx.doi.org/10.4103/JCD.JCD_518_18 Text en Copyright: © 2019 Journal of Conservative Dentistry http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Halkai, Kiran R.
Mudda, Jayashree A.
Shivanna, Vasundhara
Patil, Veena
Rathod, Vandana
Halkai, Rahul
Cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: An in vitro study
title Cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: An in vitro study
title_full Cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: An in vitro study
title_fullStr Cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: An in vitro study
title_full_unstemmed Cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: An in vitro study
title_short Cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: An in vitro study
title_sort cytotoxicity evaluation of fungal-derived silver nanoparticles on human gingival fibroblast cell line: an in vitro study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519193/
https://www.ncbi.nlm.nih.gov/pubmed/31142986
http://dx.doi.org/10.4103/JCD.JCD_518_18
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