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Wnt signaling reprograms metabolism in dental pulp stem cells
Human dental pulp stem cells (DPSCs) can differentiate to a wide range of different cell lineages, and share some gene expression and functional similarities with pluripotent stem cells. The stemness of DPSCs can also be pharmacologically enhanced by the activation of canonical Wnt signaling. Here,...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519273/ https://www.ncbi.nlm.nih.gov/pubmed/30549037 http://dx.doi.org/10.1002/jcp.27977 |
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author | Uribe‐Etxebarria, Véronica Agliano, Alice Unda, Fernando Ibarretxe, Gaskon |
author_facet | Uribe‐Etxebarria, Véronica Agliano, Alice Unda, Fernando Ibarretxe, Gaskon |
author_sort | Uribe‐Etxebarria, Véronica |
collection | PubMed |
description | Human dental pulp stem cells (DPSCs) can differentiate to a wide range of different cell lineages, and share some gene expression and functional similarities with pluripotent stem cells. The stemness of DPSCs can also be pharmacologically enhanced by the activation of canonical Wnt signaling. Here, we examined the metabolic profile of DPSCs during reprogramming linked to Wnt activation, by a short (48 hr) exposure to either the GSK3‐β inhibitor BIO (6‐bromoindirubin‐3´‐oxine) or human recombinant protein WNT‐3A. Both treatments largely increased glucose consumption, and induced a gene overexpression of pyruvate and mitochondrial acetyl‐coA producing enzymes, thus activating mitochondrial tricarboxylic acid cycle (TCA) metabolism in DPSCs. This ultimately led to an accumulation of reducing power and a mitochondrial hyperpolarization in DPSCs. Interestingly, Nile Red staining showed that lipid fuel reserves were being stored in Wnt‐activated DPSCs. We associate this metabolic reprogramming with an energy‐priming state allowing DPSCs to better respond to subsequent high demands of energy and biosynthesis metabolites for cellular growth. These results show that enhancement of the stemness of DPSCs by Wnt activation comes along with a profound metabolic remodeling, which is distinctly characterized by a crucial participation of mitochondrial metabolism. |
format | Online Article Text |
id | pubmed-6519273 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-65192732019-05-23 Wnt signaling reprograms metabolism in dental pulp stem cells Uribe‐Etxebarria, Véronica Agliano, Alice Unda, Fernando Ibarretxe, Gaskon J Cell Physiol Original Research Articles Human dental pulp stem cells (DPSCs) can differentiate to a wide range of different cell lineages, and share some gene expression and functional similarities with pluripotent stem cells. The stemness of DPSCs can also be pharmacologically enhanced by the activation of canonical Wnt signaling. Here, we examined the metabolic profile of DPSCs during reprogramming linked to Wnt activation, by a short (48 hr) exposure to either the GSK3‐β inhibitor BIO (6‐bromoindirubin‐3´‐oxine) or human recombinant protein WNT‐3A. Both treatments largely increased glucose consumption, and induced a gene overexpression of pyruvate and mitochondrial acetyl‐coA producing enzymes, thus activating mitochondrial tricarboxylic acid cycle (TCA) metabolism in DPSCs. This ultimately led to an accumulation of reducing power and a mitochondrial hyperpolarization in DPSCs. Interestingly, Nile Red staining showed that lipid fuel reserves were being stored in Wnt‐activated DPSCs. We associate this metabolic reprogramming with an energy‐priming state allowing DPSCs to better respond to subsequent high demands of energy and biosynthesis metabolites for cellular growth. These results show that enhancement of the stemness of DPSCs by Wnt activation comes along with a profound metabolic remodeling, which is distinctly characterized by a crucial participation of mitochondrial metabolism. John Wiley and Sons Inc. 2018-12-13 2019-08 /pmc/articles/PMC6519273/ /pubmed/30549037 http://dx.doi.org/10.1002/jcp.27977 Text en © 2018 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Articles Uribe‐Etxebarria, Véronica Agliano, Alice Unda, Fernando Ibarretxe, Gaskon Wnt signaling reprograms metabolism in dental pulp stem cells |
title | Wnt signaling reprograms metabolism in dental pulp stem cells |
title_full | Wnt signaling reprograms metabolism in dental pulp stem cells |
title_fullStr | Wnt signaling reprograms metabolism in dental pulp stem cells |
title_full_unstemmed | Wnt signaling reprograms metabolism in dental pulp stem cells |
title_short | Wnt signaling reprograms metabolism in dental pulp stem cells |
title_sort | wnt signaling reprograms metabolism in dental pulp stem cells |
topic | Original Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519273/ https://www.ncbi.nlm.nih.gov/pubmed/30549037 http://dx.doi.org/10.1002/jcp.27977 |
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