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Cell line-specific efficacy of thermoradiotherapy in human and canine cancer cells in vitro
OBJECTIVE: Aims were to investigate sensitivity of various human and canine cancer cell lines to hyperthermia and the influence of particular treatment conditions, and to analyze the DNA-damage response and mode of cell death in cell line radiosensitized by hyperthermia. Additionally, we were intere...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519812/ https://www.ncbi.nlm.nih.gov/pubmed/31091255 http://dx.doi.org/10.1371/journal.pone.0216744 |
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author | Nytko, Katarzyna J. Thumser-Henner, Pauline Weyland, Mathias S. Scheidegger, Stephan Bley, Carla Rohrer |
author_facet | Nytko, Katarzyna J. Thumser-Henner, Pauline Weyland, Mathias S. Scheidegger, Stephan Bley, Carla Rohrer |
author_sort | Nytko, Katarzyna J. |
collection | PubMed |
description | OBJECTIVE: Aims were to investigate sensitivity of various human and canine cancer cell lines to hyperthermia and the influence of particular treatment conditions, and to analyze the DNA-damage response and mode of cell death in cell line radiosensitized by hyperthermia. Additionally, we were interested in the involvement of HSP70 in radiosensitization. METHODS: Radiosensitization by hyperthermia was determined in a panel of human and canine cancer cell lines using clonogenic cell survival assay, as well as levels of heat shock proteins (HSPs) using immunoblotting. The influence of the hyperthermia-radiotherapy time gap, different temperatures and the order of treatments on clonogenicity of hyperthermia-sensitive A549 cells was investigated. Additionally, DNA damage and cell death were assessed by Comet assay and an apoptosis/necrosis assay. Further we induced transient knockdown in A549 cells to test HSP70’s involvement in radiosensitization. RESULTS: Out of eight cell lines tested, only two (A549 and Abrams) showed significant decrease in clonogenic cell survival when pre-treated with hyperthermia at 42°C. Strong induction of HSP70 upon thermoradiotherapy (HT-RT) treatment was found in all cell lines. Transient knockdown of HSP70 in A549 cells did not result in decrease of clonogenic cell survival in response to HT-RT. CONCLUSION: Tumor cell-type, temperature and order of treatment play an important role in radiosensitization by hyperthermia. However, hyperthermia has limited potency to radiosensitize canine cancer cells grown in a 2D cell culture setting presented here. DNA damage and apoptosis/necrosis did not increase upon combined treatment and cytosolic levels of HSP70 appear not to play critical role in the radiosensitization of A549 cells. |
format | Online Article Text |
id | pubmed-6519812 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-65198122019-05-31 Cell line-specific efficacy of thermoradiotherapy in human and canine cancer cells in vitro Nytko, Katarzyna J. Thumser-Henner, Pauline Weyland, Mathias S. Scheidegger, Stephan Bley, Carla Rohrer PLoS One Research Article OBJECTIVE: Aims were to investigate sensitivity of various human and canine cancer cell lines to hyperthermia and the influence of particular treatment conditions, and to analyze the DNA-damage response and mode of cell death in cell line radiosensitized by hyperthermia. Additionally, we were interested in the involvement of HSP70 in radiosensitization. METHODS: Radiosensitization by hyperthermia was determined in a panel of human and canine cancer cell lines using clonogenic cell survival assay, as well as levels of heat shock proteins (HSPs) using immunoblotting. The influence of the hyperthermia-radiotherapy time gap, different temperatures and the order of treatments on clonogenicity of hyperthermia-sensitive A549 cells was investigated. Additionally, DNA damage and cell death were assessed by Comet assay and an apoptosis/necrosis assay. Further we induced transient knockdown in A549 cells to test HSP70’s involvement in radiosensitization. RESULTS: Out of eight cell lines tested, only two (A549 and Abrams) showed significant decrease in clonogenic cell survival when pre-treated with hyperthermia at 42°C. Strong induction of HSP70 upon thermoradiotherapy (HT-RT) treatment was found in all cell lines. Transient knockdown of HSP70 in A549 cells did not result in decrease of clonogenic cell survival in response to HT-RT. CONCLUSION: Tumor cell-type, temperature and order of treatment play an important role in radiosensitization by hyperthermia. However, hyperthermia has limited potency to radiosensitize canine cancer cells grown in a 2D cell culture setting presented here. DNA damage and apoptosis/necrosis did not increase upon combined treatment and cytosolic levels of HSP70 appear not to play critical role in the radiosensitization of A549 cells. Public Library of Science 2019-05-15 /pmc/articles/PMC6519812/ /pubmed/31091255 http://dx.doi.org/10.1371/journal.pone.0216744 Text en © 2019 Nytko et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Nytko, Katarzyna J. Thumser-Henner, Pauline Weyland, Mathias S. Scheidegger, Stephan Bley, Carla Rohrer Cell line-specific efficacy of thermoradiotherapy in human and canine cancer cells in vitro |
title | Cell line-specific efficacy of thermoradiotherapy in human and canine cancer cells in vitro |
title_full | Cell line-specific efficacy of thermoradiotherapy in human and canine cancer cells in vitro |
title_fullStr | Cell line-specific efficacy of thermoradiotherapy in human and canine cancer cells in vitro |
title_full_unstemmed | Cell line-specific efficacy of thermoradiotherapy in human and canine cancer cells in vitro |
title_short | Cell line-specific efficacy of thermoradiotherapy in human and canine cancer cells in vitro |
title_sort | cell line-specific efficacy of thermoradiotherapy in human and canine cancer cells in vitro |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519812/ https://www.ncbi.nlm.nih.gov/pubmed/31091255 http://dx.doi.org/10.1371/journal.pone.0216744 |
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