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Animal model of arthritis and myositis induced by the Mayaro virus

BACKGROUND: The Mayaro virus (MAYV) is an endemic arbovirus in South American countries, where it is responsible for sporadic outbreaks of Mayaro fever. Clinical manifestations include fever, headache, ocular pain, rash, myalgia, and debilitating and persistent polyarthralgia. Understanding the mech...

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Autores principales: Santos, Franciele Martins, Dias, Roberto Sousa, de Oliveira, Michelle Dias, Costa, Isabella Cristina Toledo Alves, Fernandes, Luciana de Souza, Pessoa, Carine Ribeiro, da Matta, Sérgio Luis Pinto, Costa, Vivian Vasconcelos, Souza, Danielle G., da Silva, Cynthia Canêdo, de Paula, Sérgio Oliveira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519846/
https://www.ncbi.nlm.nih.gov/pubmed/31050676
http://dx.doi.org/10.1371/journal.pntd.0007375
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author Santos, Franciele Martins
Dias, Roberto Sousa
de Oliveira, Michelle Dias
Costa, Isabella Cristina Toledo Alves
Fernandes, Luciana de Souza
Pessoa, Carine Ribeiro
da Matta, Sérgio Luis Pinto
Costa, Vivian Vasconcelos
Souza, Danielle G.
da Silva, Cynthia Canêdo
de Paula, Sérgio Oliveira
author_facet Santos, Franciele Martins
Dias, Roberto Sousa
de Oliveira, Michelle Dias
Costa, Isabella Cristina Toledo Alves
Fernandes, Luciana de Souza
Pessoa, Carine Ribeiro
da Matta, Sérgio Luis Pinto
Costa, Vivian Vasconcelos
Souza, Danielle G.
da Silva, Cynthia Canêdo
de Paula, Sérgio Oliveira
author_sort Santos, Franciele Martins
collection PubMed
description BACKGROUND: The Mayaro virus (MAYV) is an endemic arbovirus in South American countries, where it is responsible for sporadic outbreaks of Mayaro fever. Clinical manifestations include fever, headache, ocular pain, rash, myalgia, and debilitating and persistent polyarthralgia. Understanding the mechanisms associated with MAYV-induced arthritis is of great importance due to the potential for its emergence, urbanization and dispersion to other regions. METHODS: 15-day old Balb/c mice were infected by two distinct pathways, below the forelimb and in the rear footpad. Animals were observed for a period of 21 days. During this time, they were monitored every 24 hours for disease signs, such as weight loss and muscle weakness. Histological damage in the muscles and joints was evaluated 3, 7, 10, 15 and 20 days post-infection. The cytokine profile in serum and muscles during MAYV infection was evaluated by flow cytometry at different post-infection times. For pain analysis, the animals were submitted to the von Frey test and titre in different organs was evaluated throughout the study to obtain viral kinetics. FINDINGS: Infection by two distinct pathways, below the forelimb and in the rear footpad, resulted in a homogeneous viral spread and the development of acute disease in animals. Clinical signs were observed such as ruffled fur, hunched posture, eye irritation and slight gait alteration. In the physical test, both groups presented loss of resistance, which was associated with histopathological damage, including myositis, arthritis, tenosynovitis and periostitis. The immune response was characterized by a strong inflammatory response mediated by the cytokines TNF-α, IL-6 and INF-γ and chemokine MCP-1, followed by the action of IL-10 and IL-4 cytokines. INTERPRETATION: The results showed that Balb/c mice represent a promising model to study mechanisms involved in MAYV pathogenesis and for future antiviral testing.
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spelling pubmed-65198462019-05-31 Animal model of arthritis and myositis induced by the Mayaro virus Santos, Franciele Martins Dias, Roberto Sousa de Oliveira, Michelle Dias Costa, Isabella Cristina Toledo Alves Fernandes, Luciana de Souza Pessoa, Carine Ribeiro da Matta, Sérgio Luis Pinto Costa, Vivian Vasconcelos Souza, Danielle G. da Silva, Cynthia Canêdo de Paula, Sérgio Oliveira PLoS Negl Trop Dis Research Article BACKGROUND: The Mayaro virus (MAYV) is an endemic arbovirus in South American countries, where it is responsible for sporadic outbreaks of Mayaro fever. Clinical manifestations include fever, headache, ocular pain, rash, myalgia, and debilitating and persistent polyarthralgia. Understanding the mechanisms associated with MAYV-induced arthritis is of great importance due to the potential for its emergence, urbanization and dispersion to other regions. METHODS: 15-day old Balb/c mice were infected by two distinct pathways, below the forelimb and in the rear footpad. Animals were observed for a period of 21 days. During this time, they were monitored every 24 hours for disease signs, such as weight loss and muscle weakness. Histological damage in the muscles and joints was evaluated 3, 7, 10, 15 and 20 days post-infection. The cytokine profile in serum and muscles during MAYV infection was evaluated by flow cytometry at different post-infection times. For pain analysis, the animals were submitted to the von Frey test and titre in different organs was evaluated throughout the study to obtain viral kinetics. FINDINGS: Infection by two distinct pathways, below the forelimb and in the rear footpad, resulted in a homogeneous viral spread and the development of acute disease in animals. Clinical signs were observed such as ruffled fur, hunched posture, eye irritation and slight gait alteration. In the physical test, both groups presented loss of resistance, which was associated with histopathological damage, including myositis, arthritis, tenosynovitis and periostitis. The immune response was characterized by a strong inflammatory response mediated by the cytokines TNF-α, IL-6 and INF-γ and chemokine MCP-1, followed by the action of IL-10 and IL-4 cytokines. INTERPRETATION: The results showed that Balb/c mice represent a promising model to study mechanisms involved in MAYV pathogenesis and for future antiviral testing. Public Library of Science 2019-05-03 /pmc/articles/PMC6519846/ /pubmed/31050676 http://dx.doi.org/10.1371/journal.pntd.0007375 Text en © 2019 Santos et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Santos, Franciele Martins
Dias, Roberto Sousa
de Oliveira, Michelle Dias
Costa, Isabella Cristina Toledo Alves
Fernandes, Luciana de Souza
Pessoa, Carine Ribeiro
da Matta, Sérgio Luis Pinto
Costa, Vivian Vasconcelos
Souza, Danielle G.
da Silva, Cynthia Canêdo
de Paula, Sérgio Oliveira
Animal model of arthritis and myositis induced by the Mayaro virus
title Animal model of arthritis and myositis induced by the Mayaro virus
title_full Animal model of arthritis and myositis induced by the Mayaro virus
title_fullStr Animal model of arthritis and myositis induced by the Mayaro virus
title_full_unstemmed Animal model of arthritis and myositis induced by the Mayaro virus
title_short Animal model of arthritis and myositis induced by the Mayaro virus
title_sort animal model of arthritis and myositis induced by the mayaro virus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519846/
https://www.ncbi.nlm.nih.gov/pubmed/31050676
http://dx.doi.org/10.1371/journal.pntd.0007375
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