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In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta

Here, we present a method for in-depth human plasma proteome analysis based on high-resolution isoelectric focusing HiRIEF LC-MS/MS, demonstrating high proteome coverage, reproducibility and the potential for liquid biopsy protein profiling. By integrating genomic sequence information to the MS-base...

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Autores principales: Pernemalm, Maria, Sandberg, AnnSofi, Zhu, Yafeng, Boekel, Jorrit, Tamburro, Davide, Schwenk, Jochen M, Björk, Albin, Wahren-Herlenius, Marie, Åmark, Hanna, Östenson, Claes-Göran, Westgren, Magnus, Lehtiö, Janne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519984/
https://www.ncbi.nlm.nih.gov/pubmed/30958262
http://dx.doi.org/10.7554/eLife.41608
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author Pernemalm, Maria
Sandberg, AnnSofi
Zhu, Yafeng
Boekel, Jorrit
Tamburro, Davide
Schwenk, Jochen M
Björk, Albin
Wahren-Herlenius, Marie
Åmark, Hanna
Östenson, Claes-Göran
Westgren, Magnus
Lehtiö, Janne
author_facet Pernemalm, Maria
Sandberg, AnnSofi
Zhu, Yafeng
Boekel, Jorrit
Tamburro, Davide
Schwenk, Jochen M
Björk, Albin
Wahren-Herlenius, Marie
Åmark, Hanna
Östenson, Claes-Göran
Westgren, Magnus
Lehtiö, Janne
author_sort Pernemalm, Maria
collection PubMed
description Here, we present a method for in-depth human plasma proteome analysis based on high-resolution isoelectric focusing HiRIEF LC-MS/MS, demonstrating high proteome coverage, reproducibility and the potential for liquid biopsy protein profiling. By integrating genomic sequence information to the MS-based plasma proteome analysis, we enable detection of single amino acid variants and for the first time demonstrate transfer of multiple protein variants between mother and fetus across the placenta. We further show that our method has the ability to detect both low abundance tissue-annotated proteins and phosphorylated proteins in plasma, as well as quantitate differences in plasma proteomes between the mother and the newborn as well as changes related to pregnancy.
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spelling pubmed-65199842019-05-16 In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta Pernemalm, Maria Sandberg, AnnSofi Zhu, Yafeng Boekel, Jorrit Tamburro, Davide Schwenk, Jochen M Björk, Albin Wahren-Herlenius, Marie Åmark, Hanna Östenson, Claes-Göran Westgren, Magnus Lehtiö, Janne eLife Computational and Systems Biology Here, we present a method for in-depth human plasma proteome analysis based on high-resolution isoelectric focusing HiRIEF LC-MS/MS, demonstrating high proteome coverage, reproducibility and the potential for liquid biopsy protein profiling. By integrating genomic sequence information to the MS-based plasma proteome analysis, we enable detection of single amino acid variants and for the first time demonstrate transfer of multiple protein variants between mother and fetus across the placenta. We further show that our method has the ability to detect both low abundance tissue-annotated proteins and phosphorylated proteins in plasma, as well as quantitate differences in plasma proteomes between the mother and the newborn as well as changes related to pregnancy. eLife Sciences Publications, Ltd 2019-04-08 /pmc/articles/PMC6519984/ /pubmed/30958262 http://dx.doi.org/10.7554/eLife.41608 Text en © 2019, Pernemalm et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Computational and Systems Biology
Pernemalm, Maria
Sandberg, AnnSofi
Zhu, Yafeng
Boekel, Jorrit
Tamburro, Davide
Schwenk, Jochen M
Björk, Albin
Wahren-Herlenius, Marie
Åmark, Hanna
Östenson, Claes-Göran
Westgren, Magnus
Lehtiö, Janne
In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta
title In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta
title_full In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta
title_fullStr In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta
title_full_unstemmed In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta
title_short In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta
title_sort in-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta
topic Computational and Systems Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519984/
https://www.ncbi.nlm.nih.gov/pubmed/30958262
http://dx.doi.org/10.7554/eLife.41608
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