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In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta
Here, we present a method for in-depth human plasma proteome analysis based on high-resolution isoelectric focusing HiRIEF LC-MS/MS, demonstrating high proteome coverage, reproducibility and the potential for liquid biopsy protein profiling. By integrating genomic sequence information to the MS-base...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519984/ https://www.ncbi.nlm.nih.gov/pubmed/30958262 http://dx.doi.org/10.7554/eLife.41608 |
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author | Pernemalm, Maria Sandberg, AnnSofi Zhu, Yafeng Boekel, Jorrit Tamburro, Davide Schwenk, Jochen M Björk, Albin Wahren-Herlenius, Marie Åmark, Hanna Östenson, Claes-Göran Westgren, Magnus Lehtiö, Janne |
author_facet | Pernemalm, Maria Sandberg, AnnSofi Zhu, Yafeng Boekel, Jorrit Tamburro, Davide Schwenk, Jochen M Björk, Albin Wahren-Herlenius, Marie Åmark, Hanna Östenson, Claes-Göran Westgren, Magnus Lehtiö, Janne |
author_sort | Pernemalm, Maria |
collection | PubMed |
description | Here, we present a method for in-depth human plasma proteome analysis based on high-resolution isoelectric focusing HiRIEF LC-MS/MS, demonstrating high proteome coverage, reproducibility and the potential for liquid biopsy protein profiling. By integrating genomic sequence information to the MS-based plasma proteome analysis, we enable detection of single amino acid variants and for the first time demonstrate transfer of multiple protein variants between mother and fetus across the placenta. We further show that our method has the ability to detect both low abundance tissue-annotated proteins and phosphorylated proteins in plasma, as well as quantitate differences in plasma proteomes between the mother and the newborn as well as changes related to pregnancy. |
format | Online Article Text |
id | pubmed-6519984 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-65199842019-05-16 In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta Pernemalm, Maria Sandberg, AnnSofi Zhu, Yafeng Boekel, Jorrit Tamburro, Davide Schwenk, Jochen M Björk, Albin Wahren-Herlenius, Marie Åmark, Hanna Östenson, Claes-Göran Westgren, Magnus Lehtiö, Janne eLife Computational and Systems Biology Here, we present a method for in-depth human plasma proteome analysis based on high-resolution isoelectric focusing HiRIEF LC-MS/MS, demonstrating high proteome coverage, reproducibility and the potential for liquid biopsy protein profiling. By integrating genomic sequence information to the MS-based plasma proteome analysis, we enable detection of single amino acid variants and for the first time demonstrate transfer of multiple protein variants between mother and fetus across the placenta. We further show that our method has the ability to detect both low abundance tissue-annotated proteins and phosphorylated proteins in plasma, as well as quantitate differences in plasma proteomes between the mother and the newborn as well as changes related to pregnancy. eLife Sciences Publications, Ltd 2019-04-08 /pmc/articles/PMC6519984/ /pubmed/30958262 http://dx.doi.org/10.7554/eLife.41608 Text en © 2019, Pernemalm et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Computational and Systems Biology Pernemalm, Maria Sandberg, AnnSofi Zhu, Yafeng Boekel, Jorrit Tamburro, Davide Schwenk, Jochen M Björk, Albin Wahren-Herlenius, Marie Åmark, Hanna Östenson, Claes-Göran Westgren, Magnus Lehtiö, Janne In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta |
title | In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta |
title_full | In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta |
title_fullStr | In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta |
title_full_unstemmed | In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta |
title_short | In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta |
title_sort | in-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta |
topic | Computational and Systems Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519984/ https://www.ncbi.nlm.nih.gov/pubmed/30958262 http://dx.doi.org/10.7554/eLife.41608 |
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