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Loss of PRC1 activity in different stem cell compartments activates a common transcriptional program with cell type–dependent outcomes

Polycomb repressive complexes are evolutionarily conserved complexes that maintain transcriptional repression during development and differentiation to establish and preserve cell identity. We recently described the fundamental role of PRC1 in preserving intestinal stem cell identity through the inh...

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Autores principales: Pivetti, Silvia, Fernandez-Perez, Daniel, D’Ambrosio, Alessandro, Barbieri, Caterina Maria, Manganaro, Daria, Rossi, Alessandra, Barnabei, Laura, Zanotti, Marika, Scelfo, Andrea, Chiacchiera, Fulvio, Pasini, Diego
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520019/
https://www.ncbi.nlm.nih.gov/pubmed/31106267
http://dx.doi.org/10.1126/sciadv.aav1594
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author Pivetti, Silvia
Fernandez-Perez, Daniel
D’Ambrosio, Alessandro
Barbieri, Caterina Maria
Manganaro, Daria
Rossi, Alessandra
Barnabei, Laura
Zanotti, Marika
Scelfo, Andrea
Chiacchiera, Fulvio
Pasini, Diego
author_facet Pivetti, Silvia
Fernandez-Perez, Daniel
D’Ambrosio, Alessandro
Barbieri, Caterina Maria
Manganaro, Daria
Rossi, Alessandra
Barnabei, Laura
Zanotti, Marika
Scelfo, Andrea
Chiacchiera, Fulvio
Pasini, Diego
author_sort Pivetti, Silvia
collection PubMed
description Polycomb repressive complexes are evolutionarily conserved complexes that maintain transcriptional repression during development and differentiation to establish and preserve cell identity. We recently described the fundamental role of PRC1 in preserving intestinal stem cell identity through the inhibition of non–lineage-specific transcription factors. To further elucidate the role of PRC1 in adult stem cell maintenance, we now investigated its role in LGR5(+) hair follicle stem cells during regeneration. We show that PRC1 depletion severely affects hair regeneration and, different from intestinal stem cells, derepression of its targets induces the ectopic activation of an epidermal-specific program. Our data support a general role of PRC1 in preserving stem cell identity that is shared between different compartments. However, the final outcome of the ectopic activation of non–lineage-specific transcription factors observed upon loss of PRC1 is largely context-dependent and likely related to the transcription factors repertoire and specific epigenetic landscape of different cellular compartments.
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spelling pubmed-65200192019-05-18 Loss of PRC1 activity in different stem cell compartments activates a common transcriptional program with cell type–dependent outcomes Pivetti, Silvia Fernandez-Perez, Daniel D’Ambrosio, Alessandro Barbieri, Caterina Maria Manganaro, Daria Rossi, Alessandra Barnabei, Laura Zanotti, Marika Scelfo, Andrea Chiacchiera, Fulvio Pasini, Diego Sci Adv Research Articles Polycomb repressive complexes are evolutionarily conserved complexes that maintain transcriptional repression during development and differentiation to establish and preserve cell identity. We recently described the fundamental role of PRC1 in preserving intestinal stem cell identity through the inhibition of non–lineage-specific transcription factors. To further elucidate the role of PRC1 in adult stem cell maintenance, we now investigated its role in LGR5(+) hair follicle stem cells during regeneration. We show that PRC1 depletion severely affects hair regeneration and, different from intestinal stem cells, derepression of its targets induces the ectopic activation of an epidermal-specific program. Our data support a general role of PRC1 in preserving stem cell identity that is shared between different compartments. However, the final outcome of the ectopic activation of non–lineage-specific transcription factors observed upon loss of PRC1 is largely context-dependent and likely related to the transcription factors repertoire and specific epigenetic landscape of different cellular compartments. American Association for the Advancement of Science 2019-05-15 /pmc/articles/PMC6520019/ /pubmed/31106267 http://dx.doi.org/10.1126/sciadv.aav1594 Text en Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Pivetti, Silvia
Fernandez-Perez, Daniel
D’Ambrosio, Alessandro
Barbieri, Caterina Maria
Manganaro, Daria
Rossi, Alessandra
Barnabei, Laura
Zanotti, Marika
Scelfo, Andrea
Chiacchiera, Fulvio
Pasini, Diego
Loss of PRC1 activity in different stem cell compartments activates a common transcriptional program with cell type–dependent outcomes
title Loss of PRC1 activity in different stem cell compartments activates a common transcriptional program with cell type–dependent outcomes
title_full Loss of PRC1 activity in different stem cell compartments activates a common transcriptional program with cell type–dependent outcomes
title_fullStr Loss of PRC1 activity in different stem cell compartments activates a common transcriptional program with cell type–dependent outcomes
title_full_unstemmed Loss of PRC1 activity in different stem cell compartments activates a common transcriptional program with cell type–dependent outcomes
title_short Loss of PRC1 activity in different stem cell compartments activates a common transcriptional program with cell type–dependent outcomes
title_sort loss of prc1 activity in different stem cell compartments activates a common transcriptional program with cell type–dependent outcomes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520019/
https://www.ncbi.nlm.nih.gov/pubmed/31106267
http://dx.doi.org/10.1126/sciadv.aav1594
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