Cargando…
In Vivo Delivery of a DNA-Encoded Monoclonal Antibody Protects Non-human Primates against Zika Virus
Zika virus (ZIKV) infection is endemic to several world regions, and many others are at high risk for seasonal outbreaks. Synthetic DNA-encoded monoclonal antibody (DMAb) is an approach that enables in vivo delivery of highly potent mAbs to control infections. We engineered DMAb-ZK190, encoding the...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Society of Gene & Cell Therapy
2019
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520333/ https://www.ncbi.nlm.nih.gov/pubmed/30962164 http://dx.doi.org/10.1016/j.ymthe.2019.03.005 |
| _version_ | 1783418719619252224 |
|---|---|
| author | Esquivel, Rianne N. Patel, Ami Kudchodkar, Sagar B. Park, Daniel H. Stettler, Karin Beltramello, Martina Allen, Jeffrey W. Mendoza, Janess Ramos, Stephanie Choi, Hyeree Borole, Piyush Asija, Kanika Bah, Mamadou Shaheen, Shareef Chen, Jing Yan, Jian Durham, Amy C. Smith, Trevor R.F. Broderick, Kate Guibinga, Ghiabe Muthumani, Kar Corti, Davide Humeau, Laurent Weiner, David B. |
| author_facet | Esquivel, Rianne N. Patel, Ami Kudchodkar, Sagar B. Park, Daniel H. Stettler, Karin Beltramello, Martina Allen, Jeffrey W. Mendoza, Janess Ramos, Stephanie Choi, Hyeree Borole, Piyush Asija, Kanika Bah, Mamadou Shaheen, Shareef Chen, Jing Yan, Jian Durham, Amy C. Smith, Trevor R.F. Broderick, Kate Guibinga, Ghiabe Muthumani, Kar Corti, Davide Humeau, Laurent Weiner, David B. |
| author_sort | Esquivel, Rianne N. |
| collection | PubMed |
| description | Zika virus (ZIKV) infection is endemic to several world regions, and many others are at high risk for seasonal outbreaks. Synthetic DNA-encoded monoclonal antibody (DMAb) is an approach that enables in vivo delivery of highly potent mAbs to control infections. We engineered DMAb-ZK190, encoding the mAb ZK190 neutralizing antibody, which targets the ZIKV E protein DIII domain. In vivo-delivered DMAb-ZK190 achieved expression levels persisting >10 weeks in mice and >3 weeks in non-human primate (NHPs), which is protective against ZIKV infectious challenge. This study is the first demonstration of infectious disease control in NHPs following in vivo delivery of a nucleic acid-encoded antibody, supporting the importance of this new platform. |
| format | Online Article Text |
| id | pubmed-6520333 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2019 |
| publisher | American Society of Gene & Cell Therapy |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-65203332020-05-08 In Vivo Delivery of a DNA-Encoded Monoclonal Antibody Protects Non-human Primates against Zika Virus Esquivel, Rianne N. Patel, Ami Kudchodkar, Sagar B. Park, Daniel H. Stettler, Karin Beltramello, Martina Allen, Jeffrey W. Mendoza, Janess Ramos, Stephanie Choi, Hyeree Borole, Piyush Asija, Kanika Bah, Mamadou Shaheen, Shareef Chen, Jing Yan, Jian Durham, Amy C. Smith, Trevor R.F. Broderick, Kate Guibinga, Ghiabe Muthumani, Kar Corti, Davide Humeau, Laurent Weiner, David B. Mol Ther Original Article Zika virus (ZIKV) infection is endemic to several world regions, and many others are at high risk for seasonal outbreaks. Synthetic DNA-encoded monoclonal antibody (DMAb) is an approach that enables in vivo delivery of highly potent mAbs to control infections. We engineered DMAb-ZK190, encoding the mAb ZK190 neutralizing antibody, which targets the ZIKV E protein DIII domain. In vivo-delivered DMAb-ZK190 achieved expression levels persisting >10 weeks in mice and >3 weeks in non-human primate (NHPs), which is protective against ZIKV infectious challenge. This study is the first demonstration of infectious disease control in NHPs following in vivo delivery of a nucleic acid-encoded antibody, supporting the importance of this new platform. American Society of Gene & Cell Therapy 2019-05-08 2019-04-05 /pmc/articles/PMC6520333/ /pubmed/30962164 http://dx.doi.org/10.1016/j.ymthe.2019.03.005 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| spellingShingle | Original Article Esquivel, Rianne N. Patel, Ami Kudchodkar, Sagar B. Park, Daniel H. Stettler, Karin Beltramello, Martina Allen, Jeffrey W. Mendoza, Janess Ramos, Stephanie Choi, Hyeree Borole, Piyush Asija, Kanika Bah, Mamadou Shaheen, Shareef Chen, Jing Yan, Jian Durham, Amy C. Smith, Trevor R.F. Broderick, Kate Guibinga, Ghiabe Muthumani, Kar Corti, Davide Humeau, Laurent Weiner, David B. In Vivo Delivery of a DNA-Encoded Monoclonal Antibody Protects Non-human Primates against Zika Virus |
| title | In Vivo Delivery of a DNA-Encoded Monoclonal Antibody Protects Non-human Primates against Zika Virus |
| title_full | In Vivo Delivery of a DNA-Encoded Monoclonal Antibody Protects Non-human Primates against Zika Virus |
| title_fullStr | In Vivo Delivery of a DNA-Encoded Monoclonal Antibody Protects Non-human Primates against Zika Virus |
| title_full_unstemmed | In Vivo Delivery of a DNA-Encoded Monoclonal Antibody Protects Non-human Primates against Zika Virus |
| title_short | In Vivo Delivery of a DNA-Encoded Monoclonal Antibody Protects Non-human Primates against Zika Virus |
| title_sort | in vivo delivery of a dna-encoded monoclonal antibody protects non-human primates against zika virus |
| topic | Original Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520333/ https://www.ncbi.nlm.nih.gov/pubmed/30962164 http://dx.doi.org/10.1016/j.ymthe.2019.03.005 |
| work_keys_str_mv | AT esquivelriannen invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT patelami invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT kudchodkarsagarb invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT parkdanielh invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT stettlerkarin invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT beltramellomartina invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT allenjeffreyw invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT mendozajaness invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT ramosstephanie invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT choihyeree invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT borolepiyush invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT asijakanika invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT bahmamadou invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT shaheenshareef invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT chenjing invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT yanjian invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT durhamamyc invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT smithtrevorrf invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT broderickkate invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT guibingaghiabe invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT muthumanikar invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT cortidavide invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT humeaulaurent invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus AT weinerdavidb invivodeliveryofadnaencodedmonoclonalantibodyprotectsnonhumanprimatesagainstzikavirus |