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Microbial Population Changes in Decaying Ascophyllum nodosum Result in Macroalgal-Polysaccharide-Degrading Bacteria with Potential Applicability in Enzyme-Assisted Extraction Technologies

Seaweeds are of significant interest in the food, pharmaceutical, and agricultural industries as they contain several commercially relevant bioactive compounds. Current extraction methods for macroalgal-derived metabolites are, however, problematic due to the complexity of the algal cell wall which...

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Autores principales: Ihua, Maureen W., Guihéneuf, Freddy, Mohammed, Halimah, Margassery, Lekha M., Jackson, Stephen A., Stengel, Dagmar B., Clarke, David J., Dobson, Alan D. W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520818/
https://www.ncbi.nlm.nih.gov/pubmed/30934874
http://dx.doi.org/10.3390/md17040200
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author Ihua, Maureen W.
Guihéneuf, Freddy
Mohammed, Halimah
Margassery, Lekha M.
Jackson, Stephen A.
Stengel, Dagmar B.
Clarke, David J.
Dobson, Alan D. W.
author_facet Ihua, Maureen W.
Guihéneuf, Freddy
Mohammed, Halimah
Margassery, Lekha M.
Jackson, Stephen A.
Stengel, Dagmar B.
Clarke, David J.
Dobson, Alan D. W.
author_sort Ihua, Maureen W.
collection PubMed
description Seaweeds are of significant interest in the food, pharmaceutical, and agricultural industries as they contain several commercially relevant bioactive compounds. Current extraction methods for macroalgal-derived metabolites are, however, problematic due to the complexity of the algal cell wall which hinders extraction efficiencies. The use of advanced extraction methods, such as enzyme-assisted extraction (EAE), which involve the application of commercial algal cell wall degrading enzymes to hydrolyze the cell wall carbohydrate network, are becoming more popular. Ascophyllum nodosum samples were collected from the Irish coast and incubated in artificial seawater for six weeks at three different temperatures (18 °C, 25 °C, and 30 °C) to induce decay. Microbial communities associated with the intact and decaying macroalga were examined using Illumina sequencing and culture-dependent approaches, including the novel ichip device. The bacterial populations associated with the seaweed were observed to change markedly upon decay. Over 800 bacterial isolates cultured from the macroalga were screened for the production of algal cell wall polysaccharidases and a range of species which displayed multiple hydrolytic enzyme activities were identified. Extracts from these enzyme-active bacterial isolates were then used in EAE of phenolics from Fucus vesiculosus and were shown to be more efficient than commercial enzyme preparations in their extraction efficiencies.
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spelling pubmed-65208182019-06-03 Microbial Population Changes in Decaying Ascophyllum nodosum Result in Macroalgal-Polysaccharide-Degrading Bacteria with Potential Applicability in Enzyme-Assisted Extraction Technologies Ihua, Maureen W. Guihéneuf, Freddy Mohammed, Halimah Margassery, Lekha M. Jackson, Stephen A. Stengel, Dagmar B. Clarke, David J. Dobson, Alan D. W. Mar Drugs Article Seaweeds are of significant interest in the food, pharmaceutical, and agricultural industries as they contain several commercially relevant bioactive compounds. Current extraction methods for macroalgal-derived metabolites are, however, problematic due to the complexity of the algal cell wall which hinders extraction efficiencies. The use of advanced extraction methods, such as enzyme-assisted extraction (EAE), which involve the application of commercial algal cell wall degrading enzymes to hydrolyze the cell wall carbohydrate network, are becoming more popular. Ascophyllum nodosum samples were collected from the Irish coast and incubated in artificial seawater for six weeks at three different temperatures (18 °C, 25 °C, and 30 °C) to induce decay. Microbial communities associated with the intact and decaying macroalga were examined using Illumina sequencing and culture-dependent approaches, including the novel ichip device. The bacterial populations associated with the seaweed were observed to change markedly upon decay. Over 800 bacterial isolates cultured from the macroalga were screened for the production of algal cell wall polysaccharidases and a range of species which displayed multiple hydrolytic enzyme activities were identified. Extracts from these enzyme-active bacterial isolates were then used in EAE of phenolics from Fucus vesiculosus and were shown to be more efficient than commercial enzyme preparations in their extraction efficiencies. MDPI 2019-03-29 /pmc/articles/PMC6520818/ /pubmed/30934874 http://dx.doi.org/10.3390/md17040200 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ihua, Maureen W.
Guihéneuf, Freddy
Mohammed, Halimah
Margassery, Lekha M.
Jackson, Stephen A.
Stengel, Dagmar B.
Clarke, David J.
Dobson, Alan D. W.
Microbial Population Changes in Decaying Ascophyllum nodosum Result in Macroalgal-Polysaccharide-Degrading Bacteria with Potential Applicability in Enzyme-Assisted Extraction Technologies
title Microbial Population Changes in Decaying Ascophyllum nodosum Result in Macroalgal-Polysaccharide-Degrading Bacteria with Potential Applicability in Enzyme-Assisted Extraction Technologies
title_full Microbial Population Changes in Decaying Ascophyllum nodosum Result in Macroalgal-Polysaccharide-Degrading Bacteria with Potential Applicability in Enzyme-Assisted Extraction Technologies
title_fullStr Microbial Population Changes in Decaying Ascophyllum nodosum Result in Macroalgal-Polysaccharide-Degrading Bacteria with Potential Applicability in Enzyme-Assisted Extraction Technologies
title_full_unstemmed Microbial Population Changes in Decaying Ascophyllum nodosum Result in Macroalgal-Polysaccharide-Degrading Bacteria with Potential Applicability in Enzyme-Assisted Extraction Technologies
title_short Microbial Population Changes in Decaying Ascophyllum nodosum Result in Macroalgal-Polysaccharide-Degrading Bacteria with Potential Applicability in Enzyme-Assisted Extraction Technologies
title_sort microbial population changes in decaying ascophyllum nodosum result in macroalgal-polysaccharide-degrading bacteria with potential applicability in enzyme-assisted extraction technologies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520818/
https://www.ncbi.nlm.nih.gov/pubmed/30934874
http://dx.doi.org/10.3390/md17040200
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