Cargando…
Broad-Spectrum Anti-Adhesive Coating Based on an Extracellular Polymer from a Marine Cyanobacterium
Medical device-associated infections are a major health threat, representing about half of all hospital-acquired infections. Current strategies to prevent this problem based on device coatings with antimicrobial compounds (antibiotics or antiseptics) have proven to be insufficient, often toxic, and...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520837/ https://www.ncbi.nlm.nih.gov/pubmed/31022915 http://dx.doi.org/10.3390/md17040243 |
_version_ | 1783418820900159488 |
---|---|
author | Costa, Bruna Mota, Rita Parreira, Paula Tamagnini, Paula L. Martins, M. Cristina Costa, Fabíola |
author_facet | Costa, Bruna Mota, Rita Parreira, Paula Tamagnini, Paula L. Martins, M. Cristina Costa, Fabíola |
author_sort | Costa, Bruna |
collection | PubMed |
description | Medical device-associated infections are a major health threat, representing about half of all hospital-acquired infections. Current strategies to prevent this problem based on device coatings with antimicrobial compounds (antibiotics or antiseptics) have proven to be insufficient, often toxic, and even promoting bacterial resistance. Herein, we report the development of an infection-preventive coating (CyanoCoating) produced with an extracellular polymer released by the marine cyanobacterium Cyanothece sp. CCY 0110. CyanoCoating was prepared by spin-coating and its bacterial anti-adhesive efficiency was evaluated against relevant etiological agents (Staphylococcus aureus, S. epidermidis, Pseudomonas aeruginosa and Escherichia coli) and platelets, both in the presence or absence of human plasma proteins. CyanoCoating cytotoxicity was assessed using the L929 fibroblasts cell line. CyanoCoating exhibited a smooth topography, low thickness and high hydrophilic properties with mild negative charge. The non-cytotoxic CyanoCoating prevented adhesion of all the bacteria tested (≤80%) and platelets (<87%), without inducing platelet activation (even in the presence of plasma proteins). The significant reduction in protein adsorption (<77%) confirmed its anti-adhesive properties. The development of this anti-adhesive coating is an important step towards the establishment of a new technological platform capable of preventing medical device-associated infections, without inducing thrombus formation in blood-contacting applications. |
format | Online Article Text |
id | pubmed-6520837 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65208372019-06-03 Broad-Spectrum Anti-Adhesive Coating Based on an Extracellular Polymer from a Marine Cyanobacterium Costa, Bruna Mota, Rita Parreira, Paula Tamagnini, Paula L. Martins, M. Cristina Costa, Fabíola Mar Drugs Article Medical device-associated infections are a major health threat, representing about half of all hospital-acquired infections. Current strategies to prevent this problem based on device coatings with antimicrobial compounds (antibiotics or antiseptics) have proven to be insufficient, often toxic, and even promoting bacterial resistance. Herein, we report the development of an infection-preventive coating (CyanoCoating) produced with an extracellular polymer released by the marine cyanobacterium Cyanothece sp. CCY 0110. CyanoCoating was prepared by spin-coating and its bacterial anti-adhesive efficiency was evaluated against relevant etiological agents (Staphylococcus aureus, S. epidermidis, Pseudomonas aeruginosa and Escherichia coli) and platelets, both in the presence or absence of human plasma proteins. CyanoCoating cytotoxicity was assessed using the L929 fibroblasts cell line. CyanoCoating exhibited a smooth topography, low thickness and high hydrophilic properties with mild negative charge. The non-cytotoxic CyanoCoating prevented adhesion of all the bacteria tested (≤80%) and platelets (<87%), without inducing platelet activation (even in the presence of plasma proteins). The significant reduction in protein adsorption (<77%) confirmed its anti-adhesive properties. The development of this anti-adhesive coating is an important step towards the establishment of a new technological platform capable of preventing medical device-associated infections, without inducing thrombus formation in blood-contacting applications. MDPI 2019-04-24 /pmc/articles/PMC6520837/ /pubmed/31022915 http://dx.doi.org/10.3390/md17040243 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Costa, Bruna Mota, Rita Parreira, Paula Tamagnini, Paula L. Martins, M. Cristina Costa, Fabíola Broad-Spectrum Anti-Adhesive Coating Based on an Extracellular Polymer from a Marine Cyanobacterium |
title | Broad-Spectrum Anti-Adhesive Coating Based on an Extracellular Polymer from a Marine Cyanobacterium |
title_full | Broad-Spectrum Anti-Adhesive Coating Based on an Extracellular Polymer from a Marine Cyanobacterium |
title_fullStr | Broad-Spectrum Anti-Adhesive Coating Based on an Extracellular Polymer from a Marine Cyanobacterium |
title_full_unstemmed | Broad-Spectrum Anti-Adhesive Coating Based on an Extracellular Polymer from a Marine Cyanobacterium |
title_short | Broad-Spectrum Anti-Adhesive Coating Based on an Extracellular Polymer from a Marine Cyanobacterium |
title_sort | broad-spectrum anti-adhesive coating based on an extracellular polymer from a marine cyanobacterium |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520837/ https://www.ncbi.nlm.nih.gov/pubmed/31022915 http://dx.doi.org/10.3390/md17040243 |
work_keys_str_mv | AT costabruna broadspectrumantiadhesivecoatingbasedonanextracellularpolymerfromamarinecyanobacterium AT motarita broadspectrumantiadhesivecoatingbasedonanextracellularpolymerfromamarinecyanobacterium AT parreirapaula broadspectrumantiadhesivecoatingbasedonanextracellularpolymerfromamarinecyanobacterium AT tamagninipaula broadspectrumantiadhesivecoatingbasedonanextracellularpolymerfromamarinecyanobacterium AT lmartinsmcristina broadspectrumantiadhesivecoatingbasedonanextracellularpolymerfromamarinecyanobacterium AT costafabiola broadspectrumantiadhesivecoatingbasedonanextracellularpolymerfromamarinecyanobacterium |