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Annexin II as a Dengue Virus Serotype 2 Interacting Protein Mediating Virus Interaction on Vero Cells

Recent evidence has demonstrated that dengue virus requires active filopodia formation for a successful infection. However, the cellular factor involved in the interaction has not been fully elucidated. We used a combination of virus overlay protein binding assay and LC-MS/MS, and identified annexin...

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Autores principales: Aliyu, Isah Abubakar, Ling, King-Hwa, Md Hashim, Nur Fariesha, Lam, Jia-Yong, Chee, Hui-Yee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520844/
https://www.ncbi.nlm.nih.gov/pubmed/30970587
http://dx.doi.org/10.3390/v11040335
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author Aliyu, Isah Abubakar
Ling, King-Hwa
Md Hashim, Nur Fariesha
Lam, Jia-Yong
Chee, Hui-Yee
author_facet Aliyu, Isah Abubakar
Ling, King-Hwa
Md Hashim, Nur Fariesha
Lam, Jia-Yong
Chee, Hui-Yee
author_sort Aliyu, Isah Abubakar
collection PubMed
description Recent evidence has demonstrated that dengue virus requires active filopodia formation for a successful infection. However, the cellular factor involved in the interaction has not been fully elucidated. We used a combination of virus overlay protein binding assay and LC-MS/MS, and identified annexin II as a dengue virus serotype 2 (DENV2) interacting protein on Vero cells, upon filopodia induction. Flow cytometry analysis showed annexin II on the Vero cells surface increased when DENV2 was added. The amount of annexin II in the plasma membrane fraction was reduced as the infection progressed. Antibody-mediated inhibition of infection and siRNA-mediated knockdown of annexin II expression significantly reduced DENV2 infection and production levels. Collectively, we demonstrated that annexin II is one of the host factor involved in DENV2 binding on Vero cells.
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spelling pubmed-65208442019-06-03 Annexin II as a Dengue Virus Serotype 2 Interacting Protein Mediating Virus Interaction on Vero Cells Aliyu, Isah Abubakar Ling, King-Hwa Md Hashim, Nur Fariesha Lam, Jia-Yong Chee, Hui-Yee Viruses Article Recent evidence has demonstrated that dengue virus requires active filopodia formation for a successful infection. However, the cellular factor involved in the interaction has not been fully elucidated. We used a combination of virus overlay protein binding assay and LC-MS/MS, and identified annexin II as a dengue virus serotype 2 (DENV2) interacting protein on Vero cells, upon filopodia induction. Flow cytometry analysis showed annexin II on the Vero cells surface increased when DENV2 was added. The amount of annexin II in the plasma membrane fraction was reduced as the infection progressed. Antibody-mediated inhibition of infection and siRNA-mediated knockdown of annexin II expression significantly reduced DENV2 infection and production levels. Collectively, we demonstrated that annexin II is one of the host factor involved in DENV2 binding on Vero cells. MDPI 2019-04-09 /pmc/articles/PMC6520844/ /pubmed/30970587 http://dx.doi.org/10.3390/v11040335 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aliyu, Isah Abubakar
Ling, King-Hwa
Md Hashim, Nur Fariesha
Lam, Jia-Yong
Chee, Hui-Yee
Annexin II as a Dengue Virus Serotype 2 Interacting Protein Mediating Virus Interaction on Vero Cells
title Annexin II as a Dengue Virus Serotype 2 Interacting Protein Mediating Virus Interaction on Vero Cells
title_full Annexin II as a Dengue Virus Serotype 2 Interacting Protein Mediating Virus Interaction on Vero Cells
title_fullStr Annexin II as a Dengue Virus Serotype 2 Interacting Protein Mediating Virus Interaction on Vero Cells
title_full_unstemmed Annexin II as a Dengue Virus Serotype 2 Interacting Protein Mediating Virus Interaction on Vero Cells
title_short Annexin II as a Dengue Virus Serotype 2 Interacting Protein Mediating Virus Interaction on Vero Cells
title_sort annexin ii as a dengue virus serotype 2 interacting protein mediating virus interaction on vero cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520844/
https://www.ncbi.nlm.nih.gov/pubmed/30970587
http://dx.doi.org/10.3390/v11040335
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