Cargando…
Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin
The innate immune response plays an important role in the pathophysiology of acute respiratory distress syndrome (ARDS). Glutamine (Gln) decreases lung inflammation in experimental ARDS, but its impact on the formation of extracellular traps (ETs) in the lung is unknown. In a mouse model of endotoxi...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520877/ https://www.ncbi.nlm.nih.gov/pubmed/31013737 http://dx.doi.org/10.3390/nu11040831 |
_version_ | 1783418830218854400 |
---|---|
author | de Oliveira, Gisele Pena Kitoko, Jamil Zola de Souza Lima-Gomes, Phillipe Rochael, Natália Cadaxo de Araújo, Carla Cristina Lugon, Pâmella Nowaski dos Santos, Heloísa Lopes Martins, Eduarda Gabrielle Lopes Ornellas, Felipe Mateus de Oliveira, Helena D’Anunciação Morales, Marcelo Marcos Olsen, Priscilla Christina Galina, Antônio Silva, Pedro Leme Saraiva, Elvira Maria Pelosi, Paolo Rocco, Patricia Rieken Macedo |
author_facet | de Oliveira, Gisele Pena Kitoko, Jamil Zola de Souza Lima-Gomes, Phillipe Rochael, Natália Cadaxo de Araújo, Carla Cristina Lugon, Pâmella Nowaski dos Santos, Heloísa Lopes Martins, Eduarda Gabrielle Lopes Ornellas, Felipe Mateus de Oliveira, Helena D’Anunciação Morales, Marcelo Marcos Olsen, Priscilla Christina Galina, Antônio Silva, Pedro Leme Saraiva, Elvira Maria Pelosi, Paolo Rocco, Patricia Rieken Macedo |
author_sort | de Oliveira, Gisele Pena |
collection | PubMed |
description | The innate immune response plays an important role in the pathophysiology of acute respiratory distress syndrome (ARDS). Glutamine (Gln) decreases lung inflammation in experimental ARDS, but its impact on the formation of extracellular traps (ETs) in the lung is unknown. In a mouse model of endotoxin-induced pulmonary ARDS, the effects of Gln treatment on leukocyte counts and ET content in bronchoalveolar lavage fluid (BALF), inflammatory profile in lung tissue, and lung morphofunction were evaluated in vivo. Furthermore, ET formation, reactive oxygen species (ROS) production, glutathione peroxidase (GPx), and glutathione reductase (GR) activities were tested in vitro. Our in vivo results demonstrated that Gln treatment reduced ET release (as indicated by cell-free-DNA content and myeloperoxidase activity), decreased lung inflammation (reductions in interferon-γ and increases in interleukin-10 levels), and improved lung morpho-function (decreased static lung elastance and alveolar collapse) in comparison with ARDS animals treated with saline. Moreover, Gln reduced ET and ROS formation in BALF cells stimulated with lipopolysaccharide in vitro, but it did not alter GPx or GR activity. In this model of endotoxin-induced pulmonary ARDS, treatment with Gln reduced pulmonary functional and morphological impairment, inflammation, and ET release in the lung. |
format | Online Article Text |
id | pubmed-6520877 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65208772019-05-31 Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin de Oliveira, Gisele Pena Kitoko, Jamil Zola de Souza Lima-Gomes, Phillipe Rochael, Natália Cadaxo de Araújo, Carla Cristina Lugon, Pâmella Nowaski dos Santos, Heloísa Lopes Martins, Eduarda Gabrielle Lopes Ornellas, Felipe Mateus de Oliveira, Helena D’Anunciação Morales, Marcelo Marcos Olsen, Priscilla Christina Galina, Antônio Silva, Pedro Leme Saraiva, Elvira Maria Pelosi, Paolo Rocco, Patricia Rieken Macedo Nutrients Article The innate immune response plays an important role in the pathophysiology of acute respiratory distress syndrome (ARDS). Glutamine (Gln) decreases lung inflammation in experimental ARDS, but its impact on the formation of extracellular traps (ETs) in the lung is unknown. In a mouse model of endotoxin-induced pulmonary ARDS, the effects of Gln treatment on leukocyte counts and ET content in bronchoalveolar lavage fluid (BALF), inflammatory profile in lung tissue, and lung morphofunction were evaluated in vivo. Furthermore, ET formation, reactive oxygen species (ROS) production, glutathione peroxidase (GPx), and glutathione reductase (GR) activities were tested in vitro. Our in vivo results demonstrated that Gln treatment reduced ET release (as indicated by cell-free-DNA content and myeloperoxidase activity), decreased lung inflammation (reductions in interferon-γ and increases in interleukin-10 levels), and improved lung morpho-function (decreased static lung elastance and alveolar collapse) in comparison with ARDS animals treated with saline. Moreover, Gln reduced ET and ROS formation in BALF cells stimulated with lipopolysaccharide in vitro, but it did not alter GPx or GR activity. In this model of endotoxin-induced pulmonary ARDS, treatment with Gln reduced pulmonary functional and morphological impairment, inflammation, and ET release in the lung. MDPI 2019-04-12 /pmc/articles/PMC6520877/ /pubmed/31013737 http://dx.doi.org/10.3390/nu11040831 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article de Oliveira, Gisele Pena Kitoko, Jamil Zola de Souza Lima-Gomes, Phillipe Rochael, Natália Cadaxo de Araújo, Carla Cristina Lugon, Pâmella Nowaski dos Santos, Heloísa Lopes Martins, Eduarda Gabrielle Lopes Ornellas, Felipe Mateus de Oliveira, Helena D’Anunciação Morales, Marcelo Marcos Olsen, Priscilla Christina Galina, Antônio Silva, Pedro Leme Saraiva, Elvira Maria Pelosi, Paolo Rocco, Patricia Rieken Macedo Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin |
title | Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin |
title_full | Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin |
title_fullStr | Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin |
title_full_unstemmed | Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin |
title_short | Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin |
title_sort | glutamine therapy reduces inflammation and extracellular trap release in experimental acute respiratory distress syndrome of pulmonary origin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520877/ https://www.ncbi.nlm.nih.gov/pubmed/31013737 http://dx.doi.org/10.3390/nu11040831 |
work_keys_str_mv | AT deoliveiragiselepena glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT kitokojamilzola glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT desouzalimagomesphillipe glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT rochaelnataliacadaxo glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT dearaujocarlacristina glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT lugonpamellanowaski glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT dossantosheloisalopes glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT martinseduardagabriellelopes glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT ornellasfelipemateus glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT deoliveirahelenadanunciacao glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT moralesmarcelomarcos glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT olsenpriscillachristina glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT galinaantonio glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT silvapedroleme glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT saraivaelviramaria glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT pelosipaolo glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin AT roccopatriciariekenmacedo glutaminetherapyreducesinflammationandextracellulartrapreleaseinexperimentalacuterespiratorydistresssyndromeofpulmonaryorigin |