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Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin

The innate immune response plays an important role in the pathophysiology of acute respiratory distress syndrome (ARDS). Glutamine (Gln) decreases lung inflammation in experimental ARDS, but its impact on the formation of extracellular traps (ETs) in the lung is unknown. In a mouse model of endotoxi...

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Autores principales: de Oliveira, Gisele Pena, Kitoko, Jamil Zola, de Souza Lima-Gomes, Phillipe, Rochael, Natália Cadaxo, de Araújo, Carla Cristina, Lugon, Pâmella Nowaski, dos Santos, Heloísa Lopes, Martins, Eduarda Gabrielle Lopes, Ornellas, Felipe Mateus, de Oliveira, Helena D’Anunciação, Morales, Marcelo Marcos, Olsen, Priscilla Christina, Galina, Antônio, Silva, Pedro Leme, Saraiva, Elvira Maria, Pelosi, Paolo, Rocco, Patricia Rieken Macedo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520877/
https://www.ncbi.nlm.nih.gov/pubmed/31013737
http://dx.doi.org/10.3390/nu11040831
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author de Oliveira, Gisele Pena
Kitoko, Jamil Zola
de Souza Lima-Gomes, Phillipe
Rochael, Natália Cadaxo
de Araújo, Carla Cristina
Lugon, Pâmella Nowaski
dos Santos, Heloísa Lopes
Martins, Eduarda Gabrielle Lopes
Ornellas, Felipe Mateus
de Oliveira, Helena D’Anunciação
Morales, Marcelo Marcos
Olsen, Priscilla Christina
Galina, Antônio
Silva, Pedro Leme
Saraiva, Elvira Maria
Pelosi, Paolo
Rocco, Patricia Rieken Macedo
author_facet de Oliveira, Gisele Pena
Kitoko, Jamil Zola
de Souza Lima-Gomes, Phillipe
Rochael, Natália Cadaxo
de Araújo, Carla Cristina
Lugon, Pâmella Nowaski
dos Santos, Heloísa Lopes
Martins, Eduarda Gabrielle Lopes
Ornellas, Felipe Mateus
de Oliveira, Helena D’Anunciação
Morales, Marcelo Marcos
Olsen, Priscilla Christina
Galina, Antônio
Silva, Pedro Leme
Saraiva, Elvira Maria
Pelosi, Paolo
Rocco, Patricia Rieken Macedo
author_sort de Oliveira, Gisele Pena
collection PubMed
description The innate immune response plays an important role in the pathophysiology of acute respiratory distress syndrome (ARDS). Glutamine (Gln) decreases lung inflammation in experimental ARDS, but its impact on the formation of extracellular traps (ETs) in the lung is unknown. In a mouse model of endotoxin-induced pulmonary ARDS, the effects of Gln treatment on leukocyte counts and ET content in bronchoalveolar lavage fluid (BALF), inflammatory profile in lung tissue, and lung morphofunction were evaluated in vivo. Furthermore, ET formation, reactive oxygen species (ROS) production, glutathione peroxidase (GPx), and glutathione reductase (GR) activities were tested in vitro. Our in vivo results demonstrated that Gln treatment reduced ET release (as indicated by cell-free-DNA content and myeloperoxidase activity), decreased lung inflammation (reductions in interferon-γ and increases in interleukin-10 levels), and improved lung morpho-function (decreased static lung elastance and alveolar collapse) in comparison with ARDS animals treated with saline. Moreover, Gln reduced ET and ROS formation in BALF cells stimulated with lipopolysaccharide in vitro, but it did not alter GPx or GR activity. In this model of endotoxin-induced pulmonary ARDS, treatment with Gln reduced pulmonary functional and morphological impairment, inflammation, and ET release in the lung.
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spelling pubmed-65208772019-05-31 Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin de Oliveira, Gisele Pena Kitoko, Jamil Zola de Souza Lima-Gomes, Phillipe Rochael, Natália Cadaxo de Araújo, Carla Cristina Lugon, Pâmella Nowaski dos Santos, Heloísa Lopes Martins, Eduarda Gabrielle Lopes Ornellas, Felipe Mateus de Oliveira, Helena D’Anunciação Morales, Marcelo Marcos Olsen, Priscilla Christina Galina, Antônio Silva, Pedro Leme Saraiva, Elvira Maria Pelosi, Paolo Rocco, Patricia Rieken Macedo Nutrients Article The innate immune response plays an important role in the pathophysiology of acute respiratory distress syndrome (ARDS). Glutamine (Gln) decreases lung inflammation in experimental ARDS, but its impact on the formation of extracellular traps (ETs) in the lung is unknown. In a mouse model of endotoxin-induced pulmonary ARDS, the effects of Gln treatment on leukocyte counts and ET content in bronchoalveolar lavage fluid (BALF), inflammatory profile in lung tissue, and lung morphofunction were evaluated in vivo. Furthermore, ET formation, reactive oxygen species (ROS) production, glutathione peroxidase (GPx), and glutathione reductase (GR) activities were tested in vitro. Our in vivo results demonstrated that Gln treatment reduced ET release (as indicated by cell-free-DNA content and myeloperoxidase activity), decreased lung inflammation (reductions in interferon-γ and increases in interleukin-10 levels), and improved lung morpho-function (decreased static lung elastance and alveolar collapse) in comparison with ARDS animals treated with saline. Moreover, Gln reduced ET and ROS formation in BALF cells stimulated with lipopolysaccharide in vitro, but it did not alter GPx or GR activity. In this model of endotoxin-induced pulmonary ARDS, treatment with Gln reduced pulmonary functional and morphological impairment, inflammation, and ET release in the lung. MDPI 2019-04-12 /pmc/articles/PMC6520877/ /pubmed/31013737 http://dx.doi.org/10.3390/nu11040831 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Oliveira, Gisele Pena
Kitoko, Jamil Zola
de Souza Lima-Gomes, Phillipe
Rochael, Natália Cadaxo
de Araújo, Carla Cristina
Lugon, Pâmella Nowaski
dos Santos, Heloísa Lopes
Martins, Eduarda Gabrielle Lopes
Ornellas, Felipe Mateus
de Oliveira, Helena D’Anunciação
Morales, Marcelo Marcos
Olsen, Priscilla Christina
Galina, Antônio
Silva, Pedro Leme
Saraiva, Elvira Maria
Pelosi, Paolo
Rocco, Patricia Rieken Macedo
Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin
title Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin
title_full Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin
title_fullStr Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin
title_full_unstemmed Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin
title_short Glutamine Therapy Reduces Inflammation and Extracellular Trap Release in Experimental Acute Respiratory Distress Syndrome of Pulmonary Origin
title_sort glutamine therapy reduces inflammation and extracellular trap release in experimental acute respiratory distress syndrome of pulmonary origin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520877/
https://www.ncbi.nlm.nih.gov/pubmed/31013737
http://dx.doi.org/10.3390/nu11040831
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