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Lactate Stimulates a Potential for Hypertrophy and Regeneration of Mouse Skeletal Muscle

The effects of lactate on muscle mass and regeneration were investigated using mouse skeletal muscle tissue and cultured C2C12 cells. Male C57BL/6J mice were randomly divided into (1) control, (2) lactate (1 mol/L in distilled water, 8.9 mL/g body weight)-administered, (3) cardio toxin (CTX)-injecte...

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Autores principales: Ohno, Yoshitaka, Ando, Koki, Ito, Takafumi, Suda, Yohei, Matsui, Yuki, Oyama, Akiko, Kaneko, Hikari, Yokoyama, Shingo, Egawa, Tatsuro, Goto, Katsumasa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520919/
https://www.ncbi.nlm.nih.gov/pubmed/30999708
http://dx.doi.org/10.3390/nu11040869
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author Ohno, Yoshitaka
Ando, Koki
Ito, Takafumi
Suda, Yohei
Matsui, Yuki
Oyama, Akiko
Kaneko, Hikari
Yokoyama, Shingo
Egawa, Tatsuro
Goto, Katsumasa
author_facet Ohno, Yoshitaka
Ando, Koki
Ito, Takafumi
Suda, Yohei
Matsui, Yuki
Oyama, Akiko
Kaneko, Hikari
Yokoyama, Shingo
Egawa, Tatsuro
Goto, Katsumasa
author_sort Ohno, Yoshitaka
collection PubMed
description The effects of lactate on muscle mass and regeneration were investigated using mouse skeletal muscle tissue and cultured C2C12 cells. Male C57BL/6J mice were randomly divided into (1) control, (2) lactate (1 mol/L in distilled water, 8.9 mL/g body weight)-administered, (3) cardio toxin (CTX)-injected (CX), and (4) lactate-administered after CTX-injection (LX) groups. CTX was injected into right tibialis anterior (TA) muscle before the oral administration of sodium lactate (five days/week for two weeks) to the mice. Oral lactate administration increased the muscle weight and fiber cross-sectional area, and the population of Pax7-positive nuclei in mouse TA skeletal muscle. Oral administration of lactate also facilitated the recovery process of CTX-associated injured mouse TA muscle mass accompanied with a transient increase in the population of Pax7-positive nuclei. Mouse myoblast-derived C2C12 cells were differentiated for five days to form myotubes with or without lactate administration. C2C12 myotube formation with an increase in protein content, fiber diameter, length, and myo-nuclei was stimulated by lactate. These observations suggest that lactate may be a potential molecule to stimulate muscle hypertrophy and regeneration of mouse skeletal muscle via the activation of muscle satellite cells.
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spelling pubmed-65209192019-05-31 Lactate Stimulates a Potential for Hypertrophy and Regeneration of Mouse Skeletal Muscle Ohno, Yoshitaka Ando, Koki Ito, Takafumi Suda, Yohei Matsui, Yuki Oyama, Akiko Kaneko, Hikari Yokoyama, Shingo Egawa, Tatsuro Goto, Katsumasa Nutrients Article The effects of lactate on muscle mass and regeneration were investigated using mouse skeletal muscle tissue and cultured C2C12 cells. Male C57BL/6J mice were randomly divided into (1) control, (2) lactate (1 mol/L in distilled water, 8.9 mL/g body weight)-administered, (3) cardio toxin (CTX)-injected (CX), and (4) lactate-administered after CTX-injection (LX) groups. CTX was injected into right tibialis anterior (TA) muscle before the oral administration of sodium lactate (five days/week for two weeks) to the mice. Oral lactate administration increased the muscle weight and fiber cross-sectional area, and the population of Pax7-positive nuclei in mouse TA skeletal muscle. Oral administration of lactate also facilitated the recovery process of CTX-associated injured mouse TA muscle mass accompanied with a transient increase in the population of Pax7-positive nuclei. Mouse myoblast-derived C2C12 cells were differentiated for five days to form myotubes with or without lactate administration. C2C12 myotube formation with an increase in protein content, fiber diameter, length, and myo-nuclei was stimulated by lactate. These observations suggest that lactate may be a potential molecule to stimulate muscle hypertrophy and regeneration of mouse skeletal muscle via the activation of muscle satellite cells. MDPI 2019-04-17 /pmc/articles/PMC6520919/ /pubmed/30999708 http://dx.doi.org/10.3390/nu11040869 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ohno, Yoshitaka
Ando, Koki
Ito, Takafumi
Suda, Yohei
Matsui, Yuki
Oyama, Akiko
Kaneko, Hikari
Yokoyama, Shingo
Egawa, Tatsuro
Goto, Katsumasa
Lactate Stimulates a Potential for Hypertrophy and Regeneration of Mouse Skeletal Muscle
title Lactate Stimulates a Potential for Hypertrophy and Regeneration of Mouse Skeletal Muscle
title_full Lactate Stimulates a Potential for Hypertrophy and Regeneration of Mouse Skeletal Muscle
title_fullStr Lactate Stimulates a Potential for Hypertrophy and Regeneration of Mouse Skeletal Muscle
title_full_unstemmed Lactate Stimulates a Potential for Hypertrophy and Regeneration of Mouse Skeletal Muscle
title_short Lactate Stimulates a Potential for Hypertrophy and Regeneration of Mouse Skeletal Muscle
title_sort lactate stimulates a potential for hypertrophy and regeneration of mouse skeletal muscle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520919/
https://www.ncbi.nlm.nih.gov/pubmed/30999708
http://dx.doi.org/10.3390/nu11040869
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