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Efficacy of 5-Aminolevulinic Acid in Photodynamic Detection and Photodynamic Therapy in Veterinary Medicine

5-Aminolevulinic acid (5-ALA), a commonly used photosensitizer in photodynamic detection (PDD) and therapy (PDT), is converted in situ to the established photosensitizer protoporphyrin IX (PpIX) via the heme biosynthetic pathway. To extend 5-ALA-PDT application, we evaluated the PpIX fluorescence in...

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Autores principales: Osaki, Tomohiro, Yokoe, Inoru, Sunden, Yuji, Ota, Urara, Ichikawa, Tomoki, Imazato, Hideo, Ishii, Takuya, Takahashi, Kiwamu, Ishizuka, Masahiro, Tanaka, Tohru, Li, Liming, Yamashita, Masamichi, Murahata, Yusuke, Tsuka, Takeshi, Azuma, Kazuo, Ito, Norihiko, Imagawa, Tomohiro, Okamoto, Yoshiharu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520946/
https://www.ncbi.nlm.nih.gov/pubmed/30959982
http://dx.doi.org/10.3390/cancers11040495
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author Osaki, Tomohiro
Yokoe, Inoru
Sunden, Yuji
Ota, Urara
Ichikawa, Tomoki
Imazato, Hideo
Ishii, Takuya
Takahashi, Kiwamu
Ishizuka, Masahiro
Tanaka, Tohru
Li, Liming
Yamashita, Masamichi
Murahata, Yusuke
Tsuka, Takeshi
Azuma, Kazuo
Ito, Norihiko
Imagawa, Tomohiro
Okamoto, Yoshiharu
author_facet Osaki, Tomohiro
Yokoe, Inoru
Sunden, Yuji
Ota, Urara
Ichikawa, Tomoki
Imazato, Hideo
Ishii, Takuya
Takahashi, Kiwamu
Ishizuka, Masahiro
Tanaka, Tohru
Li, Liming
Yamashita, Masamichi
Murahata, Yusuke
Tsuka, Takeshi
Azuma, Kazuo
Ito, Norihiko
Imagawa, Tomohiro
Okamoto, Yoshiharu
author_sort Osaki, Tomohiro
collection PubMed
description 5-Aminolevulinic acid (5-ALA), a commonly used photosensitizer in photodynamic detection (PDD) and therapy (PDT), is converted in situ to the established photosensitizer protoporphyrin IX (PpIX) via the heme biosynthetic pathway. To extend 5-ALA-PDT application, we evaluated the PpIX fluorescence induced by exogenous 5-ALA in various veterinary tumors and treated canine and feline tumors. 5-ALA-PDD sensitivity and specificity in the whole sample group for dogs and cats combined were 89.5 and 50%, respectively. Notably, some small tumors disappeared upon 5-ALA-PDT. Although single PDT application was not curative, repeated PDT+/−chemotherapy achieved long-term tumor control. We analyzed the relationship between intracellular PpIX concentration and 5-ALA-PDT in vitro cytotoxicity using various primary tumor cells and determined the correlation between intracellular PpIX concentration and 5-ALA transporter and metabolic enzyme mRNA expression levels. 5-ALA-PDT cytotoxicity in vitro correlated with intracellular PpIX concentration in carcinomas. Ferrochelatase mRNA expression levels strongly negatively correlated with PpIX accumulation, representing the first report of a correlation between mRNA expression related to PpIX accumulation and PpIX concentration in canine tumor cells. Our findings suggested that the results of 5-ALA-PDD might be predictive for 5-ALA-PDT therapeutic effects for carcinomas, with 5-ALA-PDT plus chemotherapy potentially increasing the probability of tumor control in veterinary medicine.
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spelling pubmed-65209462019-05-31 Efficacy of 5-Aminolevulinic Acid in Photodynamic Detection and Photodynamic Therapy in Veterinary Medicine Osaki, Tomohiro Yokoe, Inoru Sunden, Yuji Ota, Urara Ichikawa, Tomoki Imazato, Hideo Ishii, Takuya Takahashi, Kiwamu Ishizuka, Masahiro Tanaka, Tohru Li, Liming Yamashita, Masamichi Murahata, Yusuke Tsuka, Takeshi Azuma, Kazuo Ito, Norihiko Imagawa, Tomohiro Okamoto, Yoshiharu Cancers (Basel) Article 5-Aminolevulinic acid (5-ALA), a commonly used photosensitizer in photodynamic detection (PDD) and therapy (PDT), is converted in situ to the established photosensitizer protoporphyrin IX (PpIX) via the heme biosynthetic pathway. To extend 5-ALA-PDT application, we evaluated the PpIX fluorescence induced by exogenous 5-ALA in various veterinary tumors and treated canine and feline tumors. 5-ALA-PDD sensitivity and specificity in the whole sample group for dogs and cats combined were 89.5 and 50%, respectively. Notably, some small tumors disappeared upon 5-ALA-PDT. Although single PDT application was not curative, repeated PDT+/−chemotherapy achieved long-term tumor control. We analyzed the relationship between intracellular PpIX concentration and 5-ALA-PDT in vitro cytotoxicity using various primary tumor cells and determined the correlation between intracellular PpIX concentration and 5-ALA transporter and metabolic enzyme mRNA expression levels. 5-ALA-PDT cytotoxicity in vitro correlated with intracellular PpIX concentration in carcinomas. Ferrochelatase mRNA expression levels strongly negatively correlated with PpIX accumulation, representing the first report of a correlation between mRNA expression related to PpIX accumulation and PpIX concentration in canine tumor cells. Our findings suggested that the results of 5-ALA-PDD might be predictive for 5-ALA-PDT therapeutic effects for carcinomas, with 5-ALA-PDT plus chemotherapy potentially increasing the probability of tumor control in veterinary medicine. MDPI 2019-04-07 /pmc/articles/PMC6520946/ /pubmed/30959982 http://dx.doi.org/10.3390/cancers11040495 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Osaki, Tomohiro
Yokoe, Inoru
Sunden, Yuji
Ota, Urara
Ichikawa, Tomoki
Imazato, Hideo
Ishii, Takuya
Takahashi, Kiwamu
Ishizuka, Masahiro
Tanaka, Tohru
Li, Liming
Yamashita, Masamichi
Murahata, Yusuke
Tsuka, Takeshi
Azuma, Kazuo
Ito, Norihiko
Imagawa, Tomohiro
Okamoto, Yoshiharu
Efficacy of 5-Aminolevulinic Acid in Photodynamic Detection and Photodynamic Therapy in Veterinary Medicine
title Efficacy of 5-Aminolevulinic Acid in Photodynamic Detection and Photodynamic Therapy in Veterinary Medicine
title_full Efficacy of 5-Aminolevulinic Acid in Photodynamic Detection and Photodynamic Therapy in Veterinary Medicine
title_fullStr Efficacy of 5-Aminolevulinic Acid in Photodynamic Detection and Photodynamic Therapy in Veterinary Medicine
title_full_unstemmed Efficacy of 5-Aminolevulinic Acid in Photodynamic Detection and Photodynamic Therapy in Veterinary Medicine
title_short Efficacy of 5-Aminolevulinic Acid in Photodynamic Detection and Photodynamic Therapy in Veterinary Medicine
title_sort efficacy of 5-aminolevulinic acid in photodynamic detection and photodynamic therapy in veterinary medicine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520946/
https://www.ncbi.nlm.nih.gov/pubmed/30959982
http://dx.doi.org/10.3390/cancers11040495
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