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The Dengue ED3 Dot Assay, a Novel Serological Test for the Detection of Denguevirus Type-Specific Antibodies and Its Application in a Retrospective Seroprevalence Study

There are four distinct antigenic serotypes of dengue viruses (DENV-1-4). Sequential infections with different serotypes lead to cross-reactive but also serotype-specific neutralizing antibody responses. Neutralization assays are considered as gold standard for serotype-specific antibody detection....

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Autores principales: Auerswald, Heidi, Klepsch, Leonard, Schreiber, Sebastian, Hülsemann, Janne, Franzke, Kati, Kann, Simone, Y, Bunthin, Duong, Veasna, Buchy, Philippe, Schreiber, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6521013/
https://www.ncbi.nlm.nih.gov/pubmed/30934772
http://dx.doi.org/10.3390/v11040304
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author Auerswald, Heidi
Klepsch, Leonard
Schreiber, Sebastian
Hülsemann, Janne
Franzke, Kati
Kann, Simone
Y, Bunthin
Duong, Veasna
Buchy, Philippe
Schreiber, Michael
author_facet Auerswald, Heidi
Klepsch, Leonard
Schreiber, Sebastian
Hülsemann, Janne
Franzke, Kati
Kann, Simone
Y, Bunthin
Duong, Veasna
Buchy, Philippe
Schreiber, Michael
author_sort Auerswald, Heidi
collection PubMed
description There are four distinct antigenic serotypes of dengue viruses (DENV-1-4). Sequential infections with different serotypes lead to cross-reactive but also serotype-specific neutralizing antibody responses. Neutralization assays are considered as gold standard for serotype-specific antibody detection. However, for retrospective seroprevalence studies, access to large serum quantities is limited making neutralization assays well-nigh impossible. Therefore, a serological test, wasting only 10 µL serum, was developed using fusion proteins of maltose binding protein and E protein domain 3 (MBP-ED3) as antigens. Twelve MBP-ED3 antigens for DENV-1-4, three MBP-ED3 antigens for WNV, JEV, and TBEV, and MBP were dotted onto a single nitrocellulose strip. ED3 dot assay results were compared to virus neutralization and ED3 ELISA test results, showing a >90% accordance for DENV-1 and a 100% accordance for DENV-2, making the test specifically useful for DENV-1/-2 serotype-specific antibody detection. Since 2010, DENV-1 has replaced DENV-2 as the dominant serotype in Cambodia. In a retrospective cohort analysis, sera collected during the DENV-1/-2 endemic period showed a shift to DENV-2-specific antibody responses in 2012 paralleled by the decline of DENV-2 infections. Altogether, the ED3 dot assay is a serum-, time- and money-saving diagnostic tool for serotype-specific antibody detection, especially when serum samples are limited.
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spelling pubmed-65210132019-06-03 The Dengue ED3 Dot Assay, a Novel Serological Test for the Detection of Denguevirus Type-Specific Antibodies and Its Application in a Retrospective Seroprevalence Study Auerswald, Heidi Klepsch, Leonard Schreiber, Sebastian Hülsemann, Janne Franzke, Kati Kann, Simone Y, Bunthin Duong, Veasna Buchy, Philippe Schreiber, Michael Viruses Article There are four distinct antigenic serotypes of dengue viruses (DENV-1-4). Sequential infections with different serotypes lead to cross-reactive but also serotype-specific neutralizing antibody responses. Neutralization assays are considered as gold standard for serotype-specific antibody detection. However, for retrospective seroprevalence studies, access to large serum quantities is limited making neutralization assays well-nigh impossible. Therefore, a serological test, wasting only 10 µL serum, was developed using fusion proteins of maltose binding protein and E protein domain 3 (MBP-ED3) as antigens. Twelve MBP-ED3 antigens for DENV-1-4, three MBP-ED3 antigens for WNV, JEV, and TBEV, and MBP were dotted onto a single nitrocellulose strip. ED3 dot assay results were compared to virus neutralization and ED3 ELISA test results, showing a >90% accordance for DENV-1 and a 100% accordance for DENV-2, making the test specifically useful for DENV-1/-2 serotype-specific antibody detection. Since 2010, DENV-1 has replaced DENV-2 as the dominant serotype in Cambodia. In a retrospective cohort analysis, sera collected during the DENV-1/-2 endemic period showed a shift to DENV-2-specific antibody responses in 2012 paralleled by the decline of DENV-2 infections. Altogether, the ED3 dot assay is a serum-, time- and money-saving diagnostic tool for serotype-specific antibody detection, especially when serum samples are limited. MDPI 2019-03-27 /pmc/articles/PMC6521013/ /pubmed/30934772 http://dx.doi.org/10.3390/v11040304 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Auerswald, Heidi
Klepsch, Leonard
Schreiber, Sebastian
Hülsemann, Janne
Franzke, Kati
Kann, Simone
Y, Bunthin
Duong, Veasna
Buchy, Philippe
Schreiber, Michael
The Dengue ED3 Dot Assay, a Novel Serological Test for the Detection of Denguevirus Type-Specific Antibodies and Its Application in a Retrospective Seroprevalence Study
title The Dengue ED3 Dot Assay, a Novel Serological Test for the Detection of Denguevirus Type-Specific Antibodies and Its Application in a Retrospective Seroprevalence Study
title_full The Dengue ED3 Dot Assay, a Novel Serological Test for the Detection of Denguevirus Type-Specific Antibodies and Its Application in a Retrospective Seroprevalence Study
title_fullStr The Dengue ED3 Dot Assay, a Novel Serological Test for the Detection of Denguevirus Type-Specific Antibodies and Its Application in a Retrospective Seroprevalence Study
title_full_unstemmed The Dengue ED3 Dot Assay, a Novel Serological Test for the Detection of Denguevirus Type-Specific Antibodies and Its Application in a Retrospective Seroprevalence Study
title_short The Dengue ED3 Dot Assay, a Novel Serological Test for the Detection of Denguevirus Type-Specific Antibodies and Its Application in a Retrospective Seroprevalence Study
title_sort dengue ed3 dot assay, a novel serological test for the detection of denguevirus type-specific antibodies and its application in a retrospective seroprevalence study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6521013/
https://www.ncbi.nlm.nih.gov/pubmed/30934772
http://dx.doi.org/10.3390/v11040304
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