Cargando…
A Slow-Digesting Carbohydrate Diet during Rat Pregnancy Protects Offspring from Non-Alcoholic Fatty Liver Disease Risk through the Modulation of the Carbohydrate-Response Element and Sterol Regulatory Element Binding Proteins
High-fat (HF) and rapid digestive (RD) carbohydrate diets during pregnancy promote excessive adipogenesis in offspring. This effect can be corrected by diets with similar glycemic loads, but low rates of carbohydrate digestion. However, the effects of these diets on metabolic programming in the live...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6521114/ https://www.ncbi.nlm.nih.gov/pubmed/31013988 http://dx.doi.org/10.3390/nu11040844 |
_version_ | 1783418882164260864 |
---|---|
author | Salto, Rafael Manzano, Manuel Girón, María Dolores Cano, Ainara Castro, Azucena Vílchez, José Dámaso Cabrera, Elena López-Pedrosa, José María |
author_facet | Salto, Rafael Manzano, Manuel Girón, María Dolores Cano, Ainara Castro, Azucena Vílchez, José Dámaso Cabrera, Elena López-Pedrosa, José María |
author_sort | Salto, Rafael |
collection | PubMed |
description | High-fat (HF) and rapid digestive (RD) carbohydrate diets during pregnancy promote excessive adipogenesis in offspring. This effect can be corrected by diets with similar glycemic loads, but low rates of carbohydrate digestion. However, the effects of these diets on metabolic programming in the livers of offspring, and the liver metabolism contributions to adipogenesis, remain to be addressed. In this study, pregnant insulin-resistant rats were fed high-fat diets with similar glycemic loads but different rates of carbohydrate digestion, High Fat-Rapid Digestive (HF–RD) diet or High Fat-Slow Digestive (HF–SD) diet. Offspring were fed a standard diet for 10 weeks, and the impact of these diets on the metabolic and signaling pathways involved in liver fat synthesis and storage of offspring were analyzed, including liver lipidomics, glycogen and carbohydrate and lipid metabolism key enzymes and signaling pathways. Livers from animals whose mothers were fed an HF–RD diet showed higher saturated triacylglycerol deposits with lower carbon numbers and double bond contents compared with the HF–SD group. Moreover, the HF–RD group exhibited enhanced glucose transporter 2, pyruvate kinase (PK), acetyl coenzyme A carboxylase (ACC) and fatty acid (FA) synthase expression, and a decrease in pyruvate carboxylase (PyC) expression leading to an altered liver lipid profile. These parameters were normalized in the HF–SD group. The changes in lipogenic enzyme expression were parallel to changes in AktPKB phosphorylation status and nuclear expression in carbohydrate-response element and sterol regulatory element binding proteins. In conclusion, an HF–RD diet during pregnancy translates to changes in liver signaling and metabolic pathways in offspring, enhancing liver lipid storage and synthesis, and therefore non-alcoholic fatty liver disease (NAFLD) risk. These changes can be corrected by feeding an HF–SD diet during pregnancy. |
format | Online Article Text |
id | pubmed-6521114 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65211142019-05-31 A Slow-Digesting Carbohydrate Diet during Rat Pregnancy Protects Offspring from Non-Alcoholic Fatty Liver Disease Risk through the Modulation of the Carbohydrate-Response Element and Sterol Regulatory Element Binding Proteins Salto, Rafael Manzano, Manuel Girón, María Dolores Cano, Ainara Castro, Azucena Vílchez, José Dámaso Cabrera, Elena López-Pedrosa, José María Nutrients Article High-fat (HF) and rapid digestive (RD) carbohydrate diets during pregnancy promote excessive adipogenesis in offspring. This effect can be corrected by diets with similar glycemic loads, but low rates of carbohydrate digestion. However, the effects of these diets on metabolic programming in the livers of offspring, and the liver metabolism contributions to adipogenesis, remain to be addressed. In this study, pregnant insulin-resistant rats were fed high-fat diets with similar glycemic loads but different rates of carbohydrate digestion, High Fat-Rapid Digestive (HF–RD) diet or High Fat-Slow Digestive (HF–SD) diet. Offspring were fed a standard diet for 10 weeks, and the impact of these diets on the metabolic and signaling pathways involved in liver fat synthesis and storage of offspring were analyzed, including liver lipidomics, glycogen and carbohydrate and lipid metabolism key enzymes and signaling pathways. Livers from animals whose mothers were fed an HF–RD diet showed higher saturated triacylglycerol deposits with lower carbon numbers and double bond contents compared with the HF–SD group. Moreover, the HF–RD group exhibited enhanced glucose transporter 2, pyruvate kinase (PK), acetyl coenzyme A carboxylase (ACC) and fatty acid (FA) synthase expression, and a decrease in pyruvate carboxylase (PyC) expression leading to an altered liver lipid profile. These parameters were normalized in the HF–SD group. The changes in lipogenic enzyme expression were parallel to changes in AktPKB phosphorylation status and nuclear expression in carbohydrate-response element and sterol regulatory element binding proteins. In conclusion, an HF–RD diet during pregnancy translates to changes in liver signaling and metabolic pathways in offspring, enhancing liver lipid storage and synthesis, and therefore non-alcoholic fatty liver disease (NAFLD) risk. These changes can be corrected by feeding an HF–SD diet during pregnancy. MDPI 2019-04-14 /pmc/articles/PMC6521114/ /pubmed/31013988 http://dx.doi.org/10.3390/nu11040844 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Salto, Rafael Manzano, Manuel Girón, María Dolores Cano, Ainara Castro, Azucena Vílchez, José Dámaso Cabrera, Elena López-Pedrosa, José María A Slow-Digesting Carbohydrate Diet during Rat Pregnancy Protects Offspring from Non-Alcoholic Fatty Liver Disease Risk through the Modulation of the Carbohydrate-Response Element and Sterol Regulatory Element Binding Proteins |
title | A Slow-Digesting Carbohydrate Diet during Rat Pregnancy Protects Offspring from Non-Alcoholic Fatty Liver Disease Risk through the Modulation of the Carbohydrate-Response Element and Sterol Regulatory Element Binding Proteins |
title_full | A Slow-Digesting Carbohydrate Diet during Rat Pregnancy Protects Offspring from Non-Alcoholic Fatty Liver Disease Risk through the Modulation of the Carbohydrate-Response Element and Sterol Regulatory Element Binding Proteins |
title_fullStr | A Slow-Digesting Carbohydrate Diet during Rat Pregnancy Protects Offspring from Non-Alcoholic Fatty Liver Disease Risk through the Modulation of the Carbohydrate-Response Element and Sterol Regulatory Element Binding Proteins |
title_full_unstemmed | A Slow-Digesting Carbohydrate Diet during Rat Pregnancy Protects Offspring from Non-Alcoholic Fatty Liver Disease Risk through the Modulation of the Carbohydrate-Response Element and Sterol Regulatory Element Binding Proteins |
title_short | A Slow-Digesting Carbohydrate Diet during Rat Pregnancy Protects Offspring from Non-Alcoholic Fatty Liver Disease Risk through the Modulation of the Carbohydrate-Response Element and Sterol Regulatory Element Binding Proteins |
title_sort | slow-digesting carbohydrate diet during rat pregnancy protects offspring from non-alcoholic fatty liver disease risk through the modulation of the carbohydrate-response element and sterol regulatory element binding proteins |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6521114/ https://www.ncbi.nlm.nih.gov/pubmed/31013988 http://dx.doi.org/10.3390/nu11040844 |
work_keys_str_mv | AT saltorafael aslowdigestingcarbohydratedietduringratpregnancyprotectsoffspringfromnonalcoholicfattyliverdiseaseriskthroughthemodulationofthecarbohydrateresponseelementandsterolregulatoryelementbindingproteins AT manzanomanuel aslowdigestingcarbohydratedietduringratpregnancyprotectsoffspringfromnonalcoholicfattyliverdiseaseriskthroughthemodulationofthecarbohydrateresponseelementandsterolregulatoryelementbindingproteins AT gironmariadolores aslowdigestingcarbohydratedietduringratpregnancyprotectsoffspringfromnonalcoholicfattyliverdiseaseriskthroughthemodulationofthecarbohydrateresponseelementandsterolregulatoryelementbindingproteins AT canoainara aslowdigestingcarbohydratedietduringratpregnancyprotectsoffspringfromnonalcoholicfattyliverdiseaseriskthroughthemodulationofthecarbohydrateresponseelementandsterolregulatoryelementbindingproteins AT castroazucena aslowdigestingcarbohydratedietduringratpregnancyprotectsoffspringfromnonalcoholicfattyliverdiseaseriskthroughthemodulationofthecarbohydrateresponseelementandsterolregulatoryelementbindingproteins AT vilchezjosedamaso aslowdigestingcarbohydratedietduringratpregnancyprotectsoffspringfromnonalcoholicfattyliverdiseaseriskthroughthemodulationofthecarbohydrateresponseelementandsterolregulatoryelementbindingproteins AT cabreraelena aslowdigestingcarbohydratedietduringratpregnancyprotectsoffspringfromnonalcoholicfattyliverdiseaseriskthroughthemodulationofthecarbohydrateresponseelementandsterolregulatoryelementbindingproteins AT lopezpedrosajosemaria aslowdigestingcarbohydratedietduringratpregnancyprotectsoffspringfromnonalcoholicfattyliverdiseaseriskthroughthemodulationofthecarbohydrateresponseelementandsterolregulatoryelementbindingproteins AT saltorafael slowdigestingcarbohydratedietduringratpregnancyprotectsoffspringfromnonalcoholicfattyliverdiseaseriskthroughthemodulationofthecarbohydrateresponseelementandsterolregulatoryelementbindingproteins AT manzanomanuel slowdigestingcarbohydratedietduringratpregnancyprotectsoffspringfromnonalcoholicfattyliverdiseaseriskthroughthemodulationofthecarbohydrateresponseelementandsterolregulatoryelementbindingproteins AT gironmariadolores slowdigestingcarbohydratedietduringratpregnancyprotectsoffspringfromnonalcoholicfattyliverdiseaseriskthroughthemodulationofthecarbohydrateresponseelementandsterolregulatoryelementbindingproteins AT canoainara slowdigestingcarbohydratedietduringratpregnancyprotectsoffspringfromnonalcoholicfattyliverdiseaseriskthroughthemodulationofthecarbohydrateresponseelementandsterolregulatoryelementbindingproteins AT castroazucena slowdigestingcarbohydratedietduringratpregnancyprotectsoffspringfromnonalcoholicfattyliverdiseaseriskthroughthemodulationofthecarbohydrateresponseelementandsterolregulatoryelementbindingproteins AT vilchezjosedamaso slowdigestingcarbohydratedietduringratpregnancyprotectsoffspringfromnonalcoholicfattyliverdiseaseriskthroughthemodulationofthecarbohydrateresponseelementandsterolregulatoryelementbindingproteins AT cabreraelena slowdigestingcarbohydratedietduringratpregnancyprotectsoffspringfromnonalcoholicfattyliverdiseaseriskthroughthemodulationofthecarbohydrateresponseelementandsterolregulatoryelementbindingproteins AT lopezpedrosajosemaria slowdigestingcarbohydratedietduringratpregnancyprotectsoffspringfromnonalcoholicfattyliverdiseaseriskthroughthemodulationofthecarbohydrateresponseelementandsterolregulatoryelementbindingproteins |