Cargando…

Muscle and Systemic Molecular Responses to a Single Flywheel Based Iso-Inertial Training Session in Resistance-Trained Men

Growing evidence points to the effectiveness of flywheel (FW) based iso-inertial resistance training in improving physical performance capacities. However, molecular adaptations induced by FW exercises are largely unknown. Eight resistance-trained men performed 5 sets of 10 maximal squats on a FW de...

Descripción completa

Detalles Bibliográficos
Autores principales: Annibalini, Giosuè, Contarelli, Serena, Lucertini, Francesco, Guescini, Michele, Maggio, Serena, Ceccaroli, Paola, Gervasi, Marco, Ferri Marini, Carlo, Fardetti, Francesco, Grassi, Eugenio, Stocchi, Vilberto, Barbieri, Elena, Benelli, Piero
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6521220/
https://www.ncbi.nlm.nih.gov/pubmed/31143128
http://dx.doi.org/10.3389/fphys.2019.00554
_version_ 1783418906667384832
author Annibalini, Giosuè
Contarelli, Serena
Lucertini, Francesco
Guescini, Michele
Maggio, Serena
Ceccaroli, Paola
Gervasi, Marco
Ferri Marini, Carlo
Fardetti, Francesco
Grassi, Eugenio
Stocchi, Vilberto
Barbieri, Elena
Benelli, Piero
author_facet Annibalini, Giosuè
Contarelli, Serena
Lucertini, Francesco
Guescini, Michele
Maggio, Serena
Ceccaroli, Paola
Gervasi, Marco
Ferri Marini, Carlo
Fardetti, Francesco
Grassi, Eugenio
Stocchi, Vilberto
Barbieri, Elena
Benelli, Piero
author_sort Annibalini, Giosuè
collection PubMed
description Growing evidence points to the effectiveness of flywheel (FW) based iso-inertial resistance training in improving physical performance capacities. However, molecular adaptations induced by FW exercises are largely unknown. Eight resistance-trained men performed 5 sets of 10 maximal squats on a FW device. Muscle biopsies (fine needle aspiration technique) and blood samples were collected before (t0), and 2 h (t1) after FW exercise. Blood samples were additionally drawn after 24 h (t2) and 48 h (t3). Paired samples t-tests revealed significant increases, at t1, of mRNA expression of the genes involved in inflammation, in both muscle (MCP-1, TNF-α, IL-6) and peripheral blood mononuclear cells (IkB-α, MCP-1). Circulating extracellular vesicles (EVs) and EV-encapsulated miRNA levels (miR-206, miR-146a) significantly increased at t1 as well. Conversely, muscle mRNA level of genes associated with muscle growth/remodeling (IGF-1Ea, cyclin D1, myogenin) decreased at t1. One-way repeated measure ANOVAs, with Bonferroni corrected post-hoc pairwise comparisons, revealed significant increases in plasma concentrations of IL-6 (t1; t2; t3) and muscle creatine kinase (t1; t2), while IGF-1 significantly increased at t2 only. Our findings show that, even in experienced resistance trained individuals, a single FW training session modifies local and systemic markers involved in late structural remodeling and functional adaptation of skeletal muscle.
format Online
Article
Text
id pubmed-6521220
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-65212202019-05-29 Muscle and Systemic Molecular Responses to a Single Flywheel Based Iso-Inertial Training Session in Resistance-Trained Men Annibalini, Giosuè Contarelli, Serena Lucertini, Francesco Guescini, Michele Maggio, Serena Ceccaroli, Paola Gervasi, Marco Ferri Marini, Carlo Fardetti, Francesco Grassi, Eugenio Stocchi, Vilberto Barbieri, Elena Benelli, Piero Front Physiol Physiology Growing evidence points to the effectiveness of flywheel (FW) based iso-inertial resistance training in improving physical performance capacities. However, molecular adaptations induced by FW exercises are largely unknown. Eight resistance-trained men performed 5 sets of 10 maximal squats on a FW device. Muscle biopsies (fine needle aspiration technique) and blood samples were collected before (t0), and 2 h (t1) after FW exercise. Blood samples were additionally drawn after 24 h (t2) and 48 h (t3). Paired samples t-tests revealed significant increases, at t1, of mRNA expression of the genes involved in inflammation, in both muscle (MCP-1, TNF-α, IL-6) and peripheral blood mononuclear cells (IkB-α, MCP-1). Circulating extracellular vesicles (EVs) and EV-encapsulated miRNA levels (miR-206, miR-146a) significantly increased at t1 as well. Conversely, muscle mRNA level of genes associated with muscle growth/remodeling (IGF-1Ea, cyclin D1, myogenin) decreased at t1. One-way repeated measure ANOVAs, with Bonferroni corrected post-hoc pairwise comparisons, revealed significant increases in plasma concentrations of IL-6 (t1; t2; t3) and muscle creatine kinase (t1; t2), while IGF-1 significantly increased at t2 only. Our findings show that, even in experienced resistance trained individuals, a single FW training session modifies local and systemic markers involved in late structural remodeling and functional adaptation of skeletal muscle. Frontiers Media S.A. 2019-05-09 /pmc/articles/PMC6521220/ /pubmed/31143128 http://dx.doi.org/10.3389/fphys.2019.00554 Text en Copyright © 2019 Annibalini, Contarelli, Lucertini, Guescini, Maggio, Ceccaroli, Gervasi, Ferri Marini, Fardetti, Grassi, Stocchi, Barbieri and Benelli. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Annibalini, Giosuè
Contarelli, Serena
Lucertini, Francesco
Guescini, Michele
Maggio, Serena
Ceccaroli, Paola
Gervasi, Marco
Ferri Marini, Carlo
Fardetti, Francesco
Grassi, Eugenio
Stocchi, Vilberto
Barbieri, Elena
Benelli, Piero
Muscle and Systemic Molecular Responses to a Single Flywheel Based Iso-Inertial Training Session in Resistance-Trained Men
title Muscle and Systemic Molecular Responses to a Single Flywheel Based Iso-Inertial Training Session in Resistance-Trained Men
title_full Muscle and Systemic Molecular Responses to a Single Flywheel Based Iso-Inertial Training Session in Resistance-Trained Men
title_fullStr Muscle and Systemic Molecular Responses to a Single Flywheel Based Iso-Inertial Training Session in Resistance-Trained Men
title_full_unstemmed Muscle and Systemic Molecular Responses to a Single Flywheel Based Iso-Inertial Training Session in Resistance-Trained Men
title_short Muscle and Systemic Molecular Responses to a Single Flywheel Based Iso-Inertial Training Session in Resistance-Trained Men
title_sort muscle and systemic molecular responses to a single flywheel based iso-inertial training session in resistance-trained men
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6521220/
https://www.ncbi.nlm.nih.gov/pubmed/31143128
http://dx.doi.org/10.3389/fphys.2019.00554
work_keys_str_mv AT annibalinigiosue muscleandsystemicmolecularresponsestoasingleflywheelbasedisoinertialtrainingsessioninresistancetrainedmen
AT contarelliserena muscleandsystemicmolecularresponsestoasingleflywheelbasedisoinertialtrainingsessioninresistancetrainedmen
AT lucertinifrancesco muscleandsystemicmolecularresponsestoasingleflywheelbasedisoinertialtrainingsessioninresistancetrainedmen
AT guescinimichele muscleandsystemicmolecularresponsestoasingleflywheelbasedisoinertialtrainingsessioninresistancetrainedmen
AT maggioserena muscleandsystemicmolecularresponsestoasingleflywheelbasedisoinertialtrainingsessioninresistancetrainedmen
AT ceccarolipaola muscleandsystemicmolecularresponsestoasingleflywheelbasedisoinertialtrainingsessioninresistancetrainedmen
AT gervasimarco muscleandsystemicmolecularresponsestoasingleflywheelbasedisoinertialtrainingsessioninresistancetrainedmen
AT ferrimarinicarlo muscleandsystemicmolecularresponsestoasingleflywheelbasedisoinertialtrainingsessioninresistancetrainedmen
AT fardettifrancesco muscleandsystemicmolecularresponsestoasingleflywheelbasedisoinertialtrainingsessioninresistancetrainedmen
AT grassieugenio muscleandsystemicmolecularresponsestoasingleflywheelbasedisoinertialtrainingsessioninresistancetrainedmen
AT stocchivilberto muscleandsystemicmolecularresponsestoasingleflywheelbasedisoinertialtrainingsessioninresistancetrainedmen
AT barbierielena muscleandsystemicmolecularresponsestoasingleflywheelbasedisoinertialtrainingsessioninresistancetrainedmen
AT benellipiero muscleandsystemicmolecularresponsestoasingleflywheelbasedisoinertialtrainingsessioninresistancetrainedmen