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Muscle and Systemic Molecular Responses to a Single Flywheel Based Iso-Inertial Training Session in Resistance-Trained Men
Growing evidence points to the effectiveness of flywheel (FW) based iso-inertial resistance training in improving physical performance capacities. However, molecular adaptations induced by FW exercises are largely unknown. Eight resistance-trained men performed 5 sets of 10 maximal squats on a FW de...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6521220/ https://www.ncbi.nlm.nih.gov/pubmed/31143128 http://dx.doi.org/10.3389/fphys.2019.00554 |
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author | Annibalini, Giosuè Contarelli, Serena Lucertini, Francesco Guescini, Michele Maggio, Serena Ceccaroli, Paola Gervasi, Marco Ferri Marini, Carlo Fardetti, Francesco Grassi, Eugenio Stocchi, Vilberto Barbieri, Elena Benelli, Piero |
author_facet | Annibalini, Giosuè Contarelli, Serena Lucertini, Francesco Guescini, Michele Maggio, Serena Ceccaroli, Paola Gervasi, Marco Ferri Marini, Carlo Fardetti, Francesco Grassi, Eugenio Stocchi, Vilberto Barbieri, Elena Benelli, Piero |
author_sort | Annibalini, Giosuè |
collection | PubMed |
description | Growing evidence points to the effectiveness of flywheel (FW) based iso-inertial resistance training in improving physical performance capacities. However, molecular adaptations induced by FW exercises are largely unknown. Eight resistance-trained men performed 5 sets of 10 maximal squats on a FW device. Muscle biopsies (fine needle aspiration technique) and blood samples were collected before (t0), and 2 h (t1) after FW exercise. Blood samples were additionally drawn after 24 h (t2) and 48 h (t3). Paired samples t-tests revealed significant increases, at t1, of mRNA expression of the genes involved in inflammation, in both muscle (MCP-1, TNF-α, IL-6) and peripheral blood mononuclear cells (IkB-α, MCP-1). Circulating extracellular vesicles (EVs) and EV-encapsulated miRNA levels (miR-206, miR-146a) significantly increased at t1 as well. Conversely, muscle mRNA level of genes associated with muscle growth/remodeling (IGF-1Ea, cyclin D1, myogenin) decreased at t1. One-way repeated measure ANOVAs, with Bonferroni corrected post-hoc pairwise comparisons, revealed significant increases in plasma concentrations of IL-6 (t1; t2; t3) and muscle creatine kinase (t1; t2), while IGF-1 significantly increased at t2 only. Our findings show that, even in experienced resistance trained individuals, a single FW training session modifies local and systemic markers involved in late structural remodeling and functional adaptation of skeletal muscle. |
format | Online Article Text |
id | pubmed-6521220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-65212202019-05-29 Muscle and Systemic Molecular Responses to a Single Flywheel Based Iso-Inertial Training Session in Resistance-Trained Men Annibalini, Giosuè Contarelli, Serena Lucertini, Francesco Guescini, Michele Maggio, Serena Ceccaroli, Paola Gervasi, Marco Ferri Marini, Carlo Fardetti, Francesco Grassi, Eugenio Stocchi, Vilberto Barbieri, Elena Benelli, Piero Front Physiol Physiology Growing evidence points to the effectiveness of flywheel (FW) based iso-inertial resistance training in improving physical performance capacities. However, molecular adaptations induced by FW exercises are largely unknown. Eight resistance-trained men performed 5 sets of 10 maximal squats on a FW device. Muscle biopsies (fine needle aspiration technique) and blood samples were collected before (t0), and 2 h (t1) after FW exercise. Blood samples were additionally drawn after 24 h (t2) and 48 h (t3). Paired samples t-tests revealed significant increases, at t1, of mRNA expression of the genes involved in inflammation, in both muscle (MCP-1, TNF-α, IL-6) and peripheral blood mononuclear cells (IkB-α, MCP-1). Circulating extracellular vesicles (EVs) and EV-encapsulated miRNA levels (miR-206, miR-146a) significantly increased at t1 as well. Conversely, muscle mRNA level of genes associated with muscle growth/remodeling (IGF-1Ea, cyclin D1, myogenin) decreased at t1. One-way repeated measure ANOVAs, with Bonferroni corrected post-hoc pairwise comparisons, revealed significant increases in plasma concentrations of IL-6 (t1; t2; t3) and muscle creatine kinase (t1; t2), while IGF-1 significantly increased at t2 only. Our findings show that, even in experienced resistance trained individuals, a single FW training session modifies local and systemic markers involved in late structural remodeling and functional adaptation of skeletal muscle. Frontiers Media S.A. 2019-05-09 /pmc/articles/PMC6521220/ /pubmed/31143128 http://dx.doi.org/10.3389/fphys.2019.00554 Text en Copyright © 2019 Annibalini, Contarelli, Lucertini, Guescini, Maggio, Ceccaroli, Gervasi, Ferri Marini, Fardetti, Grassi, Stocchi, Barbieri and Benelli. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Annibalini, Giosuè Contarelli, Serena Lucertini, Francesco Guescini, Michele Maggio, Serena Ceccaroli, Paola Gervasi, Marco Ferri Marini, Carlo Fardetti, Francesco Grassi, Eugenio Stocchi, Vilberto Barbieri, Elena Benelli, Piero Muscle and Systemic Molecular Responses to a Single Flywheel Based Iso-Inertial Training Session in Resistance-Trained Men |
title | Muscle and Systemic Molecular Responses to a Single Flywheel Based Iso-Inertial Training Session in Resistance-Trained Men |
title_full | Muscle and Systemic Molecular Responses to a Single Flywheel Based Iso-Inertial Training Session in Resistance-Trained Men |
title_fullStr | Muscle and Systemic Molecular Responses to a Single Flywheel Based Iso-Inertial Training Session in Resistance-Trained Men |
title_full_unstemmed | Muscle and Systemic Molecular Responses to a Single Flywheel Based Iso-Inertial Training Session in Resistance-Trained Men |
title_short | Muscle and Systemic Molecular Responses to a Single Flywheel Based Iso-Inertial Training Session in Resistance-Trained Men |
title_sort | muscle and systemic molecular responses to a single flywheel based iso-inertial training session in resistance-trained men |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6521220/ https://www.ncbi.nlm.nih.gov/pubmed/31143128 http://dx.doi.org/10.3389/fphys.2019.00554 |
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