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A Stillborn Multiple Organs’ Investigation from a Maternal DENV-4 Infection: Histopathological and Inflammatory Mediators Characterization

Dengue virus (DENV) is an emerging virus involved in outbreaks in Brazil. The association between the virus and vertical transmission, with disorders in the placenta, has raised a worldwide concern. On the 29th gestational week, a pregnant woman presented severe complications due to a DENV infection...

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Autores principales: Nunes, Priscila, Nogueira, Rita, Coelho, Janice, Rodrigues, Francisco, Salomão, Natália, José, Carollina, de Carvalho, Jorge, Rabelo, Kíssila, de Azeredo, Elzinandes, Basílio-de-Oliveira, Rodrigo, Basílio-de-Oliveira, Carlos, dos Santos, Flávia, Paes, Marciano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6521294/
https://www.ncbi.nlm.nih.gov/pubmed/30986974
http://dx.doi.org/10.3390/v11040319
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author Nunes, Priscila
Nogueira, Rita
Coelho, Janice
Rodrigues, Francisco
Salomão, Natália
José, Carollina
de Carvalho, Jorge
Rabelo, Kíssila
de Azeredo, Elzinandes
Basílio-de-Oliveira, Rodrigo
Basílio-de-Oliveira, Carlos
dos Santos, Flávia
Paes, Marciano
author_facet Nunes, Priscila
Nogueira, Rita
Coelho, Janice
Rodrigues, Francisco
Salomão, Natália
José, Carollina
de Carvalho, Jorge
Rabelo, Kíssila
de Azeredo, Elzinandes
Basílio-de-Oliveira, Rodrigo
Basílio-de-Oliveira, Carlos
dos Santos, Flávia
Paes, Marciano
author_sort Nunes, Priscila
collection PubMed
description Dengue virus (DENV) is an emerging virus involved in outbreaks in Brazil. The association between the virus and vertical transmission, with disorders in the placenta, has raised a worldwide concern. On the 29th gestational week, a pregnant woman presented severe complications due to a DENV infection leading to maternal and fetus death. Postmortem analysis of fetal organs demonstrated the presence of DENV using reverse transcriptase polymerase chain reaction (RT-PCR) in the fetal brain and DENV non-structural protein 3 (NS3) staining in placenta and several peripheral fetal tissues, such as the brain, liver, lungs, and spleen. Histological analysis of the placenta and fetal organs revealed different types of tissue abnormalities, which included inflammation, hemorrhage, edema, and necrosis in placenta and tissue disorganization in the fetus, such as spongiform parenchyma, microglial inflammation, steatosis, hyalinose arteriolar, inflammatory cells in the alveolar septa, and disorganization of the lymphoid follicle. Increased cellularity (macrophage, Hofbauer cells and TCD8+ lymphocytes) and up-regulation of inflammatory mediators such as IFN-γ, TNF-α, RANTES/CCL5, MCP1/CCL2, and VEGF/R2 were detected in the liver, lung, spleen, brain, and placenta, supporting placental and fetus peripheral tissues inflammation. Maternal infection leading to the production of those vascular mediators may alter the vascular permeability, facilitating the virus entry and tissue and barrier dysfunction.
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spelling pubmed-65212942019-06-03 A Stillborn Multiple Organs’ Investigation from a Maternal DENV-4 Infection: Histopathological and Inflammatory Mediators Characterization Nunes, Priscila Nogueira, Rita Coelho, Janice Rodrigues, Francisco Salomão, Natália José, Carollina de Carvalho, Jorge Rabelo, Kíssila de Azeredo, Elzinandes Basílio-de-Oliveira, Rodrigo Basílio-de-Oliveira, Carlos dos Santos, Flávia Paes, Marciano Viruses Article Dengue virus (DENV) is an emerging virus involved in outbreaks in Brazil. The association between the virus and vertical transmission, with disorders in the placenta, has raised a worldwide concern. On the 29th gestational week, a pregnant woman presented severe complications due to a DENV infection leading to maternal and fetus death. Postmortem analysis of fetal organs demonstrated the presence of DENV using reverse transcriptase polymerase chain reaction (RT-PCR) in the fetal brain and DENV non-structural protein 3 (NS3) staining in placenta and several peripheral fetal tissues, such as the brain, liver, lungs, and spleen. Histological analysis of the placenta and fetal organs revealed different types of tissue abnormalities, which included inflammation, hemorrhage, edema, and necrosis in placenta and tissue disorganization in the fetus, such as spongiform parenchyma, microglial inflammation, steatosis, hyalinose arteriolar, inflammatory cells in the alveolar septa, and disorganization of the lymphoid follicle. Increased cellularity (macrophage, Hofbauer cells and TCD8+ lymphocytes) and up-regulation of inflammatory mediators such as IFN-γ, TNF-α, RANTES/CCL5, MCP1/CCL2, and VEGF/R2 were detected in the liver, lung, spleen, brain, and placenta, supporting placental and fetus peripheral tissues inflammation. Maternal infection leading to the production of those vascular mediators may alter the vascular permeability, facilitating the virus entry and tissue and barrier dysfunction. MDPI 2019-04-02 /pmc/articles/PMC6521294/ /pubmed/30986974 http://dx.doi.org/10.3390/v11040319 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nunes, Priscila
Nogueira, Rita
Coelho, Janice
Rodrigues, Francisco
Salomão, Natália
José, Carollina
de Carvalho, Jorge
Rabelo, Kíssila
de Azeredo, Elzinandes
Basílio-de-Oliveira, Rodrigo
Basílio-de-Oliveira, Carlos
dos Santos, Flávia
Paes, Marciano
A Stillborn Multiple Organs’ Investigation from a Maternal DENV-4 Infection: Histopathological and Inflammatory Mediators Characterization
title A Stillborn Multiple Organs’ Investigation from a Maternal DENV-4 Infection: Histopathological and Inflammatory Mediators Characterization
title_full A Stillborn Multiple Organs’ Investigation from a Maternal DENV-4 Infection: Histopathological and Inflammatory Mediators Characterization
title_fullStr A Stillborn Multiple Organs’ Investigation from a Maternal DENV-4 Infection: Histopathological and Inflammatory Mediators Characterization
title_full_unstemmed A Stillborn Multiple Organs’ Investigation from a Maternal DENV-4 Infection: Histopathological and Inflammatory Mediators Characterization
title_short A Stillborn Multiple Organs’ Investigation from a Maternal DENV-4 Infection: Histopathological and Inflammatory Mediators Characterization
title_sort stillborn multiple organs’ investigation from a maternal denv-4 infection: histopathological and inflammatory mediators characterization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6521294/
https://www.ncbi.nlm.nih.gov/pubmed/30986974
http://dx.doi.org/10.3390/v11040319
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