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The rice pds1 locus genetically interacts with partner to cause panicle exsertion defects and ectopic tillers in spikelets

BACKGROUND: Rice (Oryza sativa L.) is a staple food crop worldwide. Its yield and quality are affected by its tillering pattern and spikelet development. Although many genes involved in the vegetative and reproductive development of rice have been characterized in previous studies, the genetic mecha...

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Autores principales: Jiang, Qigui, Zeng, Yindi, Yu, Baiyang, Cen, Weijian, Lu, Siyuan, Jia, Peilong, Wang, Xuan, Qin, Baoxiang, Cai, Zhongquan, Luo, Jijing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6521401/
https://www.ncbi.nlm.nih.gov/pubmed/31092192
http://dx.doi.org/10.1186/s12870-019-1805-z
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author Jiang, Qigui
Zeng, Yindi
Yu, Baiyang
Cen, Weijian
Lu, Siyuan
Jia, Peilong
Wang, Xuan
Qin, Baoxiang
Cai, Zhongquan
Luo, Jijing
author_facet Jiang, Qigui
Zeng, Yindi
Yu, Baiyang
Cen, Weijian
Lu, Siyuan
Jia, Peilong
Wang, Xuan
Qin, Baoxiang
Cai, Zhongquan
Luo, Jijing
author_sort Jiang, Qigui
collection PubMed
description BACKGROUND: Rice (Oryza sativa L.) is a staple food crop worldwide. Its yield and quality are affected by its tillering pattern and spikelet development. Although many genes involved in the vegetative and reproductive development of rice have been characterized in previous studies, the genetic mechanisms that control axillary tillering, spikelet development, and panicle exsertion remain incompletely understood. RESULTS: Here, we characterized a novel rice recombinant inbred line (RIL), panicle exsertion defect and aberrant spikelet (pds). It was derived from a cross between two indica varieties, S142 and 430. Intriguingly, no abnormal phenotypes were observed in the parents of pds. This RIL exhibited sheathed panicles at heading stage. Still, a small number of tillers in pds plants were fully exserted from the flag leaves. Elongated sterile lemmas and rudimentary glumes (occurred occasionally) were observed in the spikelets of the exserted panicles and were transformed into palea/lemma-like structures. Furthermore, more interestingly, tillers occasionally grew from the axils of the elongated rudimentary glumes. Via genetic linkage analysis, we found that the abnormal phenotype of pds manifesting as genetic incompatibility or hybrid weakness was caused by genetic interaction between a recessive locus, pds1, which was derived from S142 and mapped to chromosome 8, and a locus pds2, which not yet mapped from 430. We fine-mapped pds1 to an approximately 55-kb interval delimited by the markers pds-4 and 8 M3.51. Six RGAP-annotated ORFs were included in this genomic region. qPCR analysis revealed that Loc_Os080595 might be the target of pds1 locus, and G1 gene might be involved in the genetic mechanism underlying the pds phenotype. CONCLUSIONS: In this study, histological and genetic analyses revealed that the pyramided pds loci resulted in genetic incompatibility or hybrid weakness in rice might be caused by a genetic interaction between pds loci derived from different rice varieties. Further isolation of pds1 and its interactor pds2, would provide new insight into the molecular regulation of grass inflorescence development and exsertion, and the evolution history of the extant rice. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-019-1805-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-65214012019-05-23 The rice pds1 locus genetically interacts with partner to cause panicle exsertion defects and ectopic tillers in spikelets Jiang, Qigui Zeng, Yindi Yu, Baiyang Cen, Weijian Lu, Siyuan Jia, Peilong Wang, Xuan Qin, Baoxiang Cai, Zhongquan Luo, Jijing BMC Plant Biol Research Article BACKGROUND: Rice (Oryza sativa L.) is a staple food crop worldwide. Its yield and quality are affected by its tillering pattern and spikelet development. Although many genes involved in the vegetative and reproductive development of rice have been characterized in previous studies, the genetic mechanisms that control axillary tillering, spikelet development, and panicle exsertion remain incompletely understood. RESULTS: Here, we characterized a novel rice recombinant inbred line (RIL), panicle exsertion defect and aberrant spikelet (pds). It was derived from a cross between two indica varieties, S142 and 430. Intriguingly, no abnormal phenotypes were observed in the parents of pds. This RIL exhibited sheathed panicles at heading stage. Still, a small number of tillers in pds plants were fully exserted from the flag leaves. Elongated sterile lemmas and rudimentary glumes (occurred occasionally) were observed in the spikelets of the exserted panicles and were transformed into palea/lemma-like structures. Furthermore, more interestingly, tillers occasionally grew from the axils of the elongated rudimentary glumes. Via genetic linkage analysis, we found that the abnormal phenotype of pds manifesting as genetic incompatibility or hybrid weakness was caused by genetic interaction between a recessive locus, pds1, which was derived from S142 and mapped to chromosome 8, and a locus pds2, which not yet mapped from 430. We fine-mapped pds1 to an approximately 55-kb interval delimited by the markers pds-4 and 8 M3.51. Six RGAP-annotated ORFs were included in this genomic region. qPCR analysis revealed that Loc_Os080595 might be the target of pds1 locus, and G1 gene might be involved in the genetic mechanism underlying the pds phenotype. CONCLUSIONS: In this study, histological and genetic analyses revealed that the pyramided pds loci resulted in genetic incompatibility or hybrid weakness in rice might be caused by a genetic interaction between pds loci derived from different rice varieties. Further isolation of pds1 and its interactor pds2, would provide new insight into the molecular regulation of grass inflorescence development and exsertion, and the evolution history of the extant rice. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-019-1805-z) contains supplementary material, which is available to authorized users. BioMed Central 2019-05-15 /pmc/articles/PMC6521401/ /pubmed/31092192 http://dx.doi.org/10.1186/s12870-019-1805-z Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Jiang, Qigui
Zeng, Yindi
Yu, Baiyang
Cen, Weijian
Lu, Siyuan
Jia, Peilong
Wang, Xuan
Qin, Baoxiang
Cai, Zhongquan
Luo, Jijing
The rice pds1 locus genetically interacts with partner to cause panicle exsertion defects and ectopic tillers in spikelets
title The rice pds1 locus genetically interacts with partner to cause panicle exsertion defects and ectopic tillers in spikelets
title_full The rice pds1 locus genetically interacts with partner to cause panicle exsertion defects and ectopic tillers in spikelets
title_fullStr The rice pds1 locus genetically interacts with partner to cause panicle exsertion defects and ectopic tillers in spikelets
title_full_unstemmed The rice pds1 locus genetically interacts with partner to cause panicle exsertion defects and ectopic tillers in spikelets
title_short The rice pds1 locus genetically interacts with partner to cause panicle exsertion defects and ectopic tillers in spikelets
title_sort rice pds1 locus genetically interacts with partner to cause panicle exsertion defects and ectopic tillers in spikelets
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6521401/
https://www.ncbi.nlm.nih.gov/pubmed/31092192
http://dx.doi.org/10.1186/s12870-019-1805-z
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