Cargando…

Remineralization ability of two hydraulic calcium-silicate based dental pulp capping materials: Cell-independent model

BACKGROUND: This study aimed to evaluate remineralizing ability of two hydraulic calcium-silicate cements (Biodentine and TheraCal LC). MATERIAL AND METHODS: Artificial carious lesions were introduced into the pulpal floors (1-1.5 mm) and axial walls of occlusal prepared cavity halves through pH cyc...

Descripción completa

Detalles Bibliográficos
Autor principal: Fathy, Salma M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medicina Oral S.L. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522110/
https://www.ncbi.nlm.nih.gov/pubmed/31110616
http://dx.doi.org/10.4317/jced.55689
_version_ 1783419077458395136
author Fathy, Salma M.
author_facet Fathy, Salma M.
author_sort Fathy, Salma M.
collection PubMed
description BACKGROUND: This study aimed to evaluate remineralizing ability of two hydraulic calcium-silicate cements (Biodentine and TheraCal LC). MATERIAL AND METHODS: Artificial carious lesions were introduced into the pulpal floors (1-1.5 mm) and axial walls of occlusal prepared cavity halves through pH cycling. Cycling was made through demineralizing solution (pH 3), for 8 hours and remineralizing solution (pH 7) for 16 hours. The total period of pH cycling was 14 days. Prepared cavities with the tested materials seated directly on the pulpal floor and in contact with the axial walls were stored in phosphate buffer solutions (PBS) (pH 7.2–7.4). The changes in the weight percentages (wt%) of calcium (Ca) and phosphorus (P) were detected using SEM and energy dispersive X-ray spectroscopy with reference to sound dentin after three intervals (one week, 3 and 6 months). Data were statistically analyzed using two-way ANOVA and Tukey’s post hoc test. RESULTS: Demineralized dentin, next to Biodentine, showed statistically higher intensities of Ca and P wt% after the three periods of incubation (p< 0.05). Surface mapping of both tested cements and their adjacent demineralized dentin showed increase in overall distribution of previous ions. SEM of subsurface layer under both materials showed filling of most intra-tubular areas with rod-like mineralized structure without significant difference. CONCLUSIONS: Biodentine has a higher ability to enrich the artificial carious dentin with significantly higher mineral contents available for remineralization. Both pulp-capping materials have significantly induced remineralization of demineralized dentin beneath them after total period of incubation. Key words:Artificial Caries, Hydraulic Cements, pH Cycling, Remineralization.
format Online
Article
Text
id pubmed-6522110
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Medicina Oral S.L.
record_format MEDLINE/PubMed
spelling pubmed-65221102019-05-20 Remineralization ability of two hydraulic calcium-silicate based dental pulp capping materials: Cell-independent model Fathy, Salma M. J Clin Exp Dent Research BACKGROUND: This study aimed to evaluate remineralizing ability of two hydraulic calcium-silicate cements (Biodentine and TheraCal LC). MATERIAL AND METHODS: Artificial carious lesions were introduced into the pulpal floors (1-1.5 mm) and axial walls of occlusal prepared cavity halves through pH cycling. Cycling was made through demineralizing solution (pH 3), for 8 hours and remineralizing solution (pH 7) for 16 hours. The total period of pH cycling was 14 days. Prepared cavities with the tested materials seated directly on the pulpal floor and in contact with the axial walls were stored in phosphate buffer solutions (PBS) (pH 7.2–7.4). The changes in the weight percentages (wt%) of calcium (Ca) and phosphorus (P) were detected using SEM and energy dispersive X-ray spectroscopy with reference to sound dentin after three intervals (one week, 3 and 6 months). Data were statistically analyzed using two-way ANOVA and Tukey’s post hoc test. RESULTS: Demineralized dentin, next to Biodentine, showed statistically higher intensities of Ca and P wt% after the three periods of incubation (p< 0.05). Surface mapping of both tested cements and their adjacent demineralized dentin showed increase in overall distribution of previous ions. SEM of subsurface layer under both materials showed filling of most intra-tubular areas with rod-like mineralized structure without significant difference. CONCLUSIONS: Biodentine has a higher ability to enrich the artificial carious dentin with significantly higher mineral contents available for remineralization. Both pulp-capping materials have significantly induced remineralization of demineralized dentin beneath them after total period of incubation. Key words:Artificial Caries, Hydraulic Cements, pH Cycling, Remineralization. Medicina Oral S.L. 2019-04-01 /pmc/articles/PMC6522110/ /pubmed/31110616 http://dx.doi.org/10.4317/jced.55689 Text en Copyright: © 2019 Medicina Oral S.L. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Fathy, Salma M.
Remineralization ability of two hydraulic calcium-silicate based dental pulp capping materials: Cell-independent model
title Remineralization ability of two hydraulic calcium-silicate based dental pulp capping materials: Cell-independent model
title_full Remineralization ability of two hydraulic calcium-silicate based dental pulp capping materials: Cell-independent model
title_fullStr Remineralization ability of two hydraulic calcium-silicate based dental pulp capping materials: Cell-independent model
title_full_unstemmed Remineralization ability of two hydraulic calcium-silicate based dental pulp capping materials: Cell-independent model
title_short Remineralization ability of two hydraulic calcium-silicate based dental pulp capping materials: Cell-independent model
title_sort remineralization ability of two hydraulic calcium-silicate based dental pulp capping materials: cell-independent model
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522110/
https://www.ncbi.nlm.nih.gov/pubmed/31110616
http://dx.doi.org/10.4317/jced.55689
work_keys_str_mv AT fathysalmam remineralizationabilityoftwohydrauliccalciumsilicatebaseddentalpulpcappingmaterialscellindependentmodel