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Detection of Type VII collagen in odontogenic keratocyst: An immunohistochemical study

BACKGROUND: Separation of the epithelial lining from the underlying connective tissue wall has been a frequently observed and unique feature in odontogenic keratocysts (OKC), but not in other odontogenic cysts nor neoplasms. No study on OKC has been reported evaluating the role of type VII Collagen,...

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Autores principales: Cota, Jochima-Eudora, Spadigam, Anita, Dhupar, Anita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medicina Oral S.L. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522116/
https://www.ncbi.nlm.nih.gov/pubmed/31110608
http://dx.doi.org/10.4317/jced.54946
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author Cota, Jochima-Eudora
Spadigam, Anita
Dhupar, Anita
author_facet Cota, Jochima-Eudora
Spadigam, Anita
Dhupar, Anita
author_sort Cota, Jochima-Eudora
collection PubMed
description BACKGROUND: Separation of the epithelial lining from the underlying connective tissue wall has been a frequently observed and unique feature in odontogenic keratocysts (OKC), but not in other odontogenic cysts nor neoplasms. No study on OKC has been reported evaluating the role of type VII Collagen, the anchoring fibrils, which function in stabilising the epithelial structure. The purpose of this study was to assess the role of type VII collagen in the fragility of the epithelium leading to a high recurrence rate in OKCs. MATERIAL AND METHODS: Immunohistochemical staining with Abcam® Monoclonal Mouse Anti-Collagen VII Antibody [LH7.2] (used at a dilution of 1:200) on 30 tissues of OKC. The chi-square test was applied to confirm the statistical significance between the control and test groups. The frequencies of the pattern of distribution for the staining characteristics of collagen VII were calculated in the OKC samples. RESULTS: Out of the 30 OKC samples 22 (73.3%) showed negative staining for type VII Collagen. Among the infected cases, 7 showed a positive basement membrane staining and one of the non-infected OKC showed positive basement membrane staining. However, none of the syndrome associated or recurrent OKCs showed any evidence of type VII collagen reactivity. CONCLUSIONS: Considering the distribution of type VII collagen in OKCs it can be concluded that type VII collagen expression is altered in OKCs, leading to destabilisation of the epithelium connective tissue interface thus rendering the epithelium-connective tissue interface fragile. Key words:Type VII Collagen, Odontogenic Keratocyst, Basal Lamina, Immunohistochemistry.
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spelling pubmed-65221162019-05-20 Detection of Type VII collagen in odontogenic keratocyst: An immunohistochemical study Cota, Jochima-Eudora Spadigam, Anita Dhupar, Anita J Clin Exp Dent Research BACKGROUND: Separation of the epithelial lining from the underlying connective tissue wall has been a frequently observed and unique feature in odontogenic keratocysts (OKC), but not in other odontogenic cysts nor neoplasms. No study on OKC has been reported evaluating the role of type VII Collagen, the anchoring fibrils, which function in stabilising the epithelial structure. The purpose of this study was to assess the role of type VII collagen in the fragility of the epithelium leading to a high recurrence rate in OKCs. MATERIAL AND METHODS: Immunohistochemical staining with Abcam® Monoclonal Mouse Anti-Collagen VII Antibody [LH7.2] (used at a dilution of 1:200) on 30 tissues of OKC. The chi-square test was applied to confirm the statistical significance between the control and test groups. The frequencies of the pattern of distribution for the staining characteristics of collagen VII were calculated in the OKC samples. RESULTS: Out of the 30 OKC samples 22 (73.3%) showed negative staining for type VII Collagen. Among the infected cases, 7 showed a positive basement membrane staining and one of the non-infected OKC showed positive basement membrane staining. However, none of the syndrome associated or recurrent OKCs showed any evidence of type VII collagen reactivity. CONCLUSIONS: Considering the distribution of type VII collagen in OKCs it can be concluded that type VII collagen expression is altered in OKCs, leading to destabilisation of the epithelium connective tissue interface thus rendering the epithelium-connective tissue interface fragile. Key words:Type VII Collagen, Odontogenic Keratocyst, Basal Lamina, Immunohistochemistry. Medicina Oral S.L. 2019-04-01 /pmc/articles/PMC6522116/ /pubmed/31110608 http://dx.doi.org/10.4317/jced.54946 Text en Copyright: © 2019 Medicina Oral S.L. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Cota, Jochima-Eudora
Spadigam, Anita
Dhupar, Anita
Detection of Type VII collagen in odontogenic keratocyst: An immunohistochemical study
title Detection of Type VII collagen in odontogenic keratocyst: An immunohistochemical study
title_full Detection of Type VII collagen in odontogenic keratocyst: An immunohistochemical study
title_fullStr Detection of Type VII collagen in odontogenic keratocyst: An immunohistochemical study
title_full_unstemmed Detection of Type VII collagen in odontogenic keratocyst: An immunohistochemical study
title_short Detection of Type VII collagen in odontogenic keratocyst: An immunohistochemical study
title_sort detection of type vii collagen in odontogenic keratocyst: an immunohistochemical study
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522116/
https://www.ncbi.nlm.nih.gov/pubmed/31110608
http://dx.doi.org/10.4317/jced.54946
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