Hsa_circ_0004370 promotes esophageal cancer progression through miR-1294/LASP1 pathway

Circular RNAs (circRNAs) formed by back-splicing play multiple roles in the occurrence and development of cancer. Here, we found that hsa_circ_0004370 was up-regulated in both esophageal cancer (EC) tissues and cell lines. Loss function of hsa_circ_0004370 by si-RNA significantly suppressed prolifer...

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Autores principales: Zhang, Zhenyang, Lin, Wenwei, Gao, Lei, Chen, Keqing, Yang, Chuangcai, Zhuang, Linwei, Peng, Shuai, Kang, Mingqiang, Lin, Jiangbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2019
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522713/
https://www.ncbi.nlm.nih.gov/pubmed/30988074
http://dx.doi.org/10.1042/BSR20182377
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author Zhang, Zhenyang
Lin, Wenwei
Gao, Lei
Chen, Keqing
Yang, Chuangcai
Zhuang, Linwei
Peng, Shuai
Kang, Mingqiang
Lin, Jiangbo
author_facet Zhang, Zhenyang
Lin, Wenwei
Gao, Lei
Chen, Keqing
Yang, Chuangcai
Zhuang, Linwei
Peng, Shuai
Kang, Mingqiang
Lin, Jiangbo
author_sort Zhang, Zhenyang
collection PubMed
description Circular RNAs (circRNAs) formed by back-splicing play multiple roles in the occurrence and development of cancer. Here, we found that hsa_circ_0004370 was up-regulated in both esophageal cancer (EC) tissues and cell lines. Loss function of hsa_circ_0004370 by si-RNA significantly suppressed proliferation and invasion and promoted apoptosis in both EC cell lines. The sponging of miR-1294 by hsa_circ_0004370 was bioinformatically predicted and subsequently verified by luciferase reporter assay and RNA immunoprecipitation assay. Further, hsa_circ_0004370 involved in the up-regulation of LASP1 by sponging miR-1294. Besides, the inhibition of the down-regulated hsa_circ_0004370 on cell malignant behaviors was rescued by miR-1294 inhibitor. Finally, this rescue effect was abrogated by suppressing the expression of LASP1. The results present here suggest that hsa_circ_0004370 functions as an oncogene on cell proliferation, apoptosis, and invasion via miR-1294/LASP1 axis.
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spelling pubmed-65227132019-05-28 Hsa_circ_0004370 promotes esophageal cancer progression through miR-1294/LASP1 pathway Zhang, Zhenyang Lin, Wenwei Gao, Lei Chen, Keqing Yang, Chuangcai Zhuang, Linwei Peng, Shuai Kang, Mingqiang Lin, Jiangbo Biosci Rep Research Articles Circular RNAs (circRNAs) formed by back-splicing play multiple roles in the occurrence and development of cancer. Here, we found that hsa_circ_0004370 was up-regulated in both esophageal cancer (EC) tissues and cell lines. Loss function of hsa_circ_0004370 by si-RNA significantly suppressed proliferation and invasion and promoted apoptosis in both EC cell lines. The sponging of miR-1294 by hsa_circ_0004370 was bioinformatically predicted and subsequently verified by luciferase reporter assay and RNA immunoprecipitation assay. Further, hsa_circ_0004370 involved in the up-regulation of LASP1 by sponging miR-1294. Besides, the inhibition of the down-regulated hsa_circ_0004370 on cell malignant behaviors was rescued by miR-1294 inhibitor. Finally, this rescue effect was abrogated by suppressing the expression of LASP1. The results present here suggest that hsa_circ_0004370 functions as an oncogene on cell proliferation, apoptosis, and invasion via miR-1294/LASP1 axis. Portland Press Ltd. 2019-05-14 /pmc/articles/PMC6522713/ /pubmed/30988074 http://dx.doi.org/10.1042/BSR20182377 Text en © 2019 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Zhang, Zhenyang
Lin, Wenwei
Gao, Lei
Chen, Keqing
Yang, Chuangcai
Zhuang, Linwei
Peng, Shuai
Kang, Mingqiang
Lin, Jiangbo
Hsa_circ_0004370 promotes esophageal cancer progression through miR-1294/LASP1 pathway
title Hsa_circ_0004370 promotes esophageal cancer progression through miR-1294/LASP1 pathway
title_full Hsa_circ_0004370 promotes esophageal cancer progression through miR-1294/LASP1 pathway
title_fullStr Hsa_circ_0004370 promotes esophageal cancer progression through miR-1294/LASP1 pathway
title_full_unstemmed Hsa_circ_0004370 promotes esophageal cancer progression through miR-1294/LASP1 pathway
title_short Hsa_circ_0004370 promotes esophageal cancer progression through miR-1294/LASP1 pathway
title_sort hsa_circ_0004370 promotes esophageal cancer progression through mir-1294/lasp1 pathway
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522713/
https://www.ncbi.nlm.nih.gov/pubmed/30988074
http://dx.doi.org/10.1042/BSR20182377
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