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Decreased expression of ubiquilin-1 following neonatal hypoxia-ischemic brain injury in mice
Ubiquilin-1 (Ubqln), a ubiquitin-like protein, regulates degradation of misfolded proteins and has been reported to have a crucial role in multiple pathologic and physiologic conditions. The current study was undertaken to investigate the expression of Ubqln in the brain of a neonatal hypoxia-ischem...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522830/ https://www.ncbi.nlm.nih.gov/pubmed/31059032 http://dx.doi.org/10.3892/mmr.2019.10168 |
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author | Luo, Li Liu, Yilin Tu, Xing Ren, Xuxin Zhao, Wenyan Liu, Jing Zhang, Li Chen, Weiqiang Zhang, Pei Wang, Weicai Lü, Lanhai Wang, Mengxia |
author_facet | Luo, Li Liu, Yilin Tu, Xing Ren, Xuxin Zhao, Wenyan Liu, Jing Zhang, Li Chen, Weiqiang Zhang, Pei Wang, Weicai Lü, Lanhai Wang, Mengxia |
author_sort | Luo, Li |
collection | PubMed |
description | Ubiquilin-1 (Ubqln), a ubiquitin-like protein, regulates degradation of misfolded proteins and has been reported to have a crucial role in multiple pathologic and physiologic conditions. The current study was undertaken to investigate the expression of Ubqln in the brain of a neonatal hypoxia-ischemic (HI) brain injury model induced using the Rice method with some modifications. Mouse pups at postnatal day 7 day were used in this study. Pups underwent permanent ligation of the left common carotid artery and a consecutive hypoxic challenge (8% O(2) and 92% N(2) for 120 min). The expression of Ubqln in the brain of pups following HI was analyzed by immunofluorescence staining and western blot analysis. Immunofluorescence staining demonstrated that Ubqln was extensively distributed in the cerebral cortex and hippocampus, and Ubqln was expressed in neurons, astrocytes and microglia in the brains of the HI brain injury model mice. Western blot analyses revealed decreased expression of Ubqln in the HI penumbra of the mouse model compared with Ubqln in the sham control group. The results of this study revealed that HI alters the expression of Ubqln, thus may provide a novel understanding of role of Ubqln in neonatal hypoxic ischemic encephalopathy. |
format | Online Article Text |
id | pubmed-6522830 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-65228302019-06-18 Decreased expression of ubiquilin-1 following neonatal hypoxia-ischemic brain injury in mice Luo, Li Liu, Yilin Tu, Xing Ren, Xuxin Zhao, Wenyan Liu, Jing Zhang, Li Chen, Weiqiang Zhang, Pei Wang, Weicai Lü, Lanhai Wang, Mengxia Mol Med Rep Articles Ubiquilin-1 (Ubqln), a ubiquitin-like protein, regulates degradation of misfolded proteins and has been reported to have a crucial role in multiple pathologic and physiologic conditions. The current study was undertaken to investigate the expression of Ubqln in the brain of a neonatal hypoxia-ischemic (HI) brain injury model induced using the Rice method with some modifications. Mouse pups at postnatal day 7 day were used in this study. Pups underwent permanent ligation of the left common carotid artery and a consecutive hypoxic challenge (8% O(2) and 92% N(2) for 120 min). The expression of Ubqln in the brain of pups following HI was analyzed by immunofluorescence staining and western blot analysis. Immunofluorescence staining demonstrated that Ubqln was extensively distributed in the cerebral cortex and hippocampus, and Ubqln was expressed in neurons, astrocytes and microglia in the brains of the HI brain injury model mice. Western blot analyses revealed decreased expression of Ubqln in the HI penumbra of the mouse model compared with Ubqln in the sham control group. The results of this study revealed that HI alters the expression of Ubqln, thus may provide a novel understanding of role of Ubqln in neonatal hypoxic ischemic encephalopathy. D.A. Spandidos 2019-06 2019-04-15 /pmc/articles/PMC6522830/ /pubmed/31059032 http://dx.doi.org/10.3892/mmr.2019.10168 Text en Copyright: © Luo et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Luo, Li Liu, Yilin Tu, Xing Ren, Xuxin Zhao, Wenyan Liu, Jing Zhang, Li Chen, Weiqiang Zhang, Pei Wang, Weicai Lü, Lanhai Wang, Mengxia Decreased expression of ubiquilin-1 following neonatal hypoxia-ischemic brain injury in mice |
title | Decreased expression of ubiquilin-1 following neonatal hypoxia-ischemic brain injury in mice |
title_full | Decreased expression of ubiquilin-1 following neonatal hypoxia-ischemic brain injury in mice |
title_fullStr | Decreased expression of ubiquilin-1 following neonatal hypoxia-ischemic brain injury in mice |
title_full_unstemmed | Decreased expression of ubiquilin-1 following neonatal hypoxia-ischemic brain injury in mice |
title_short | Decreased expression of ubiquilin-1 following neonatal hypoxia-ischemic brain injury in mice |
title_sort | decreased expression of ubiquilin-1 following neonatal hypoxia-ischemic brain injury in mice |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522830/ https://www.ncbi.nlm.nih.gov/pubmed/31059032 http://dx.doi.org/10.3892/mmr.2019.10168 |
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