Cargando…

The Dlg Module and Clathrin-Mediated Endocytosis Regulate EGFR Signaling and Cyst Cell-Germline Coordination in the Drosophila Testis

Tissue homeostasis and repair relies on proper communication of stem cells and their differentiating daughters with the local tissue microenvironment. In the Drosophila male germline adult stem cell lineage, germ cells proliferate and progressively differentiate enclosed in supportive somatic cyst c...

Descripción completa

Detalles Bibliográficos
Autores principales: Papagiannouli, Fani, Berry, Cameron Wynn, Fuller, Margaret T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523063/
https://www.ncbi.nlm.nih.gov/pubmed/31006632
http://dx.doi.org/10.1016/j.stemcr.2019.03.008
_version_ 1783419246371405824
author Papagiannouli, Fani
Berry, Cameron Wynn
Fuller, Margaret T.
author_facet Papagiannouli, Fani
Berry, Cameron Wynn
Fuller, Margaret T.
author_sort Papagiannouli, Fani
collection PubMed
description Tissue homeostasis and repair relies on proper communication of stem cells and their differentiating daughters with the local tissue microenvironment. In the Drosophila male germline adult stem cell lineage, germ cells proliferate and progressively differentiate enclosed in supportive somatic cyst cells, forming a small organoid, the functional unit of differentiation. Here we show that cell polarity and vesicle trafficking influence signal transduction in cyst cells, with profound effects on the germ cells they enclose. Our data suggest that the cortical components Dlg, Scrib, Lgl and the clathrin-mediated endocytic (CME) machinery downregulate epidermal growth factor receptor (EGFR) signaling. Knockdown of dlg, scrib, lgl, or CME components in cyst cells resulted in germ cell death, similar to increased signal transduction via the EGFR, while lowering EGFR or downstream signaling components rescued the defects. This work provides insights into how cell polarity and endocytosis cooperate to regulate signal transduction and sculpt developing tissues.
format Online
Article
Text
id pubmed-6523063
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-65230632019-05-24 The Dlg Module and Clathrin-Mediated Endocytosis Regulate EGFR Signaling and Cyst Cell-Germline Coordination in the Drosophila Testis Papagiannouli, Fani Berry, Cameron Wynn Fuller, Margaret T. Stem Cell Reports Article Tissue homeostasis and repair relies on proper communication of stem cells and their differentiating daughters with the local tissue microenvironment. In the Drosophila male germline adult stem cell lineage, germ cells proliferate and progressively differentiate enclosed in supportive somatic cyst cells, forming a small organoid, the functional unit of differentiation. Here we show that cell polarity and vesicle trafficking influence signal transduction in cyst cells, with profound effects on the germ cells they enclose. Our data suggest that the cortical components Dlg, Scrib, Lgl and the clathrin-mediated endocytic (CME) machinery downregulate epidermal growth factor receptor (EGFR) signaling. Knockdown of dlg, scrib, lgl, or CME components in cyst cells resulted in germ cell death, similar to increased signal transduction via the EGFR, while lowering EGFR or downstream signaling components rescued the defects. This work provides insights into how cell polarity and endocytosis cooperate to regulate signal transduction and sculpt developing tissues. Elsevier 2019-04-18 /pmc/articles/PMC6523063/ /pubmed/31006632 http://dx.doi.org/10.1016/j.stemcr.2019.03.008 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Papagiannouli, Fani
Berry, Cameron Wynn
Fuller, Margaret T.
The Dlg Module and Clathrin-Mediated Endocytosis Regulate EGFR Signaling and Cyst Cell-Germline Coordination in the Drosophila Testis
title The Dlg Module and Clathrin-Mediated Endocytosis Regulate EGFR Signaling and Cyst Cell-Germline Coordination in the Drosophila Testis
title_full The Dlg Module and Clathrin-Mediated Endocytosis Regulate EGFR Signaling and Cyst Cell-Germline Coordination in the Drosophila Testis
title_fullStr The Dlg Module and Clathrin-Mediated Endocytosis Regulate EGFR Signaling and Cyst Cell-Germline Coordination in the Drosophila Testis
title_full_unstemmed The Dlg Module and Clathrin-Mediated Endocytosis Regulate EGFR Signaling and Cyst Cell-Germline Coordination in the Drosophila Testis
title_short The Dlg Module and Clathrin-Mediated Endocytosis Regulate EGFR Signaling and Cyst Cell-Germline Coordination in the Drosophila Testis
title_sort dlg module and clathrin-mediated endocytosis regulate egfr signaling and cyst cell-germline coordination in the drosophila testis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523063/
https://www.ncbi.nlm.nih.gov/pubmed/31006632
http://dx.doi.org/10.1016/j.stemcr.2019.03.008
work_keys_str_mv AT papagiannoulifani thedlgmoduleandclathrinmediatedendocytosisregulateegfrsignalingandcystcellgermlinecoordinationinthedrosophilatestis
AT berrycameronwynn thedlgmoduleandclathrinmediatedendocytosisregulateegfrsignalingandcystcellgermlinecoordinationinthedrosophilatestis
AT fullermargarett thedlgmoduleandclathrinmediatedendocytosisregulateegfrsignalingandcystcellgermlinecoordinationinthedrosophilatestis
AT papagiannoulifani dlgmoduleandclathrinmediatedendocytosisregulateegfrsignalingandcystcellgermlinecoordinationinthedrosophilatestis
AT berrycameronwynn dlgmoduleandclathrinmediatedendocytosisregulateegfrsignalingandcystcellgermlinecoordinationinthedrosophilatestis
AT fullermargarett dlgmoduleandclathrinmediatedendocytosisregulateegfrsignalingandcystcellgermlinecoordinationinthedrosophilatestis