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Characterization of Brassica rapa RAP2.4-Related Proteins in Stress Response and as CUL3-Dependent E3 Ligase Substrates

The turnip Brassica rapa has important economic value and represents a good model system to study gene function in crop plants. ERF/AP2 transcription factors are a major group of proteins that are often involved in regulating stress-responses and developmental programs. Some ERF/AP2 proteins are tar...

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Autores principales: Mooney, Sutton, Al-Saharin, Raed, Choi, Christina M., Tucker, Kyle, Beathard, Chase, Hellmann, Hanjo A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523098/
https://www.ncbi.nlm.nih.gov/pubmed/30974760
http://dx.doi.org/10.3390/cells8040336
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author Mooney, Sutton
Al-Saharin, Raed
Choi, Christina M.
Tucker, Kyle
Beathard, Chase
Hellmann, Hanjo A.
author_facet Mooney, Sutton
Al-Saharin, Raed
Choi, Christina M.
Tucker, Kyle
Beathard, Chase
Hellmann, Hanjo A.
author_sort Mooney, Sutton
collection PubMed
description The turnip Brassica rapa has important economic value and represents a good model system to study gene function in crop plants. ERF/AP2 transcription factors are a major group of proteins that are often involved in regulating stress-responses and developmental programs. Some ERF/AP2 proteins are targets of CULLIN3-based E3 ligases that use BTB/POZ-MATH proteins as substrate receptors. These receptors bind the transcription factor and facilitate their ubiquitylation and subsequent degradation via the 26S proteasome. Here, we show tissue and stress-dependent expression patterns for three Brassica rapa ERF/AP2 proteins that are closely related to Arabidopsis thaliana AtRAP2.4. Cloning of the Brassica genes showed that the corresponding proteins can assemble with a BPM protein and CULLIN3, and that they are instable in a 26S proteasome dependent manner. This work demonstrates the conserved nature of the ERF/AP2-CULLIN3-based E3 ligase interplay, and represents a first step to analyze their function in a commercially relevant crop plant.
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spelling pubmed-65230982019-06-03 Characterization of Brassica rapa RAP2.4-Related Proteins in Stress Response and as CUL3-Dependent E3 Ligase Substrates Mooney, Sutton Al-Saharin, Raed Choi, Christina M. Tucker, Kyle Beathard, Chase Hellmann, Hanjo A. Cells Article The turnip Brassica rapa has important economic value and represents a good model system to study gene function in crop plants. ERF/AP2 transcription factors are a major group of proteins that are often involved in regulating stress-responses and developmental programs. Some ERF/AP2 proteins are targets of CULLIN3-based E3 ligases that use BTB/POZ-MATH proteins as substrate receptors. These receptors bind the transcription factor and facilitate their ubiquitylation and subsequent degradation via the 26S proteasome. Here, we show tissue and stress-dependent expression patterns for three Brassica rapa ERF/AP2 proteins that are closely related to Arabidopsis thaliana AtRAP2.4. Cloning of the Brassica genes showed that the corresponding proteins can assemble with a BPM protein and CULLIN3, and that they are instable in a 26S proteasome dependent manner. This work demonstrates the conserved nature of the ERF/AP2-CULLIN3-based E3 ligase interplay, and represents a first step to analyze their function in a commercially relevant crop plant. MDPI 2019-04-10 /pmc/articles/PMC6523098/ /pubmed/30974760 http://dx.doi.org/10.3390/cells8040336 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mooney, Sutton
Al-Saharin, Raed
Choi, Christina M.
Tucker, Kyle
Beathard, Chase
Hellmann, Hanjo A.
Characterization of Brassica rapa RAP2.4-Related Proteins in Stress Response and as CUL3-Dependent E3 Ligase Substrates
title Characterization of Brassica rapa RAP2.4-Related Proteins in Stress Response and as CUL3-Dependent E3 Ligase Substrates
title_full Characterization of Brassica rapa RAP2.4-Related Proteins in Stress Response and as CUL3-Dependent E3 Ligase Substrates
title_fullStr Characterization of Brassica rapa RAP2.4-Related Proteins in Stress Response and as CUL3-Dependent E3 Ligase Substrates
title_full_unstemmed Characterization of Brassica rapa RAP2.4-Related Proteins in Stress Response and as CUL3-Dependent E3 Ligase Substrates
title_short Characterization of Brassica rapa RAP2.4-Related Proteins in Stress Response and as CUL3-Dependent E3 Ligase Substrates
title_sort characterization of brassica rapa rap2.4-related proteins in stress response and as cul3-dependent e3 ligase substrates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523098/
https://www.ncbi.nlm.nih.gov/pubmed/30974760
http://dx.doi.org/10.3390/cells8040336
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