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Fe(3)O(4) Nanoparticles for Complex Targeted Delivery and Boron Neutron Capture Therapy
Magnetic Fe(3)O(4) nanoparticles (NPs) and their surface modification with therapeutic substances are of great interest, especially drug delivery for cancer therapy, including boron-neutron capture therapy (BNCT). In this paper, we present the results of boron-rich compound (carborane borate) attach...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523109/ https://www.ncbi.nlm.nih.gov/pubmed/30935156 http://dx.doi.org/10.3390/nano9040494 |
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author | Dukenbayev, Kanat Korolkov, Ilya V. Tishkevich, Daria I. Kozlovskiy, Artem L. Trukhanov, Sergey V. Gorin, Yevgeniy G. Shumskaya, Elena E. Kaniukov, Egor Y. Vinnik, Denis A. Zdorovets, Maxim V. Anisovich, Marina Trukhanov, Alex V. Tosi, Daniele Molardi, Carlo |
author_facet | Dukenbayev, Kanat Korolkov, Ilya V. Tishkevich, Daria I. Kozlovskiy, Artem L. Trukhanov, Sergey V. Gorin, Yevgeniy G. Shumskaya, Elena E. Kaniukov, Egor Y. Vinnik, Denis A. Zdorovets, Maxim V. Anisovich, Marina Trukhanov, Alex V. Tosi, Daniele Molardi, Carlo |
author_sort | Dukenbayev, Kanat |
collection | PubMed |
description | Magnetic Fe(3)O(4) nanoparticles (NPs) and their surface modification with therapeutic substances are of great interest, especially drug delivery for cancer therapy, including boron-neutron capture therapy (BNCT). In this paper, we present the results of boron-rich compound (carborane borate) attachment to previously aminated by (3-aminopropyl)-trimethoxysilane (APTMS) iron oxide NPs. Fourier transform infrared spectroscopy with Attenuated total reflectance accessory (ATR-FTIR) and energy-dispersive X-ray analysis confirmed the change of the element content of NPs after modification and formation of new bonds between Fe(3)O(4) NPs and the attached molecules. Transmission (TEM) and scanning electron microscopy (SEM) showed Fe(3)O(4) NPs’ average size of 18.9 nm. Phase parameters were studied by powder X-ray diffraction (XRD), and the magnetic behavior of Fe(3)O(4) NPs was elucidated by Mössbauer spectroscopy. The colloidal and chemical stability of NPs was studied using simulated body fluid (phosphate buffer—PBS). Modified NPs have shown excellent stability in PBS (pH = 7.4), characterized by XRD, Mössbauer spectroscopy, and dynamic light scattering (DLS). Biocompatibility was evaluated in-vitro using cultured mouse embryonic fibroblasts (MEFs). The results show us an increasing of IC(50) from 0.110 mg/mL for Fe(3)O(4) NPs to 0.405 mg/mL for Fe(3)O(4)-Carborane NPs. The obtained data confirm the biocompatibility and stability of synthesized NPs and the potential to use them in BNCT. |
format | Online Article Text |
id | pubmed-6523109 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65231092019-06-03 Fe(3)O(4) Nanoparticles for Complex Targeted Delivery and Boron Neutron Capture Therapy Dukenbayev, Kanat Korolkov, Ilya V. Tishkevich, Daria I. Kozlovskiy, Artem L. Trukhanov, Sergey V. Gorin, Yevgeniy G. Shumskaya, Elena E. Kaniukov, Egor Y. Vinnik, Denis A. Zdorovets, Maxim V. Anisovich, Marina Trukhanov, Alex V. Tosi, Daniele Molardi, Carlo Nanomaterials (Basel) Article Magnetic Fe(3)O(4) nanoparticles (NPs) and their surface modification with therapeutic substances are of great interest, especially drug delivery for cancer therapy, including boron-neutron capture therapy (BNCT). In this paper, we present the results of boron-rich compound (carborane borate) attachment to previously aminated by (3-aminopropyl)-trimethoxysilane (APTMS) iron oxide NPs. Fourier transform infrared spectroscopy with Attenuated total reflectance accessory (ATR-FTIR) and energy-dispersive X-ray analysis confirmed the change of the element content of NPs after modification and formation of new bonds between Fe(3)O(4) NPs and the attached molecules. Transmission (TEM) and scanning electron microscopy (SEM) showed Fe(3)O(4) NPs’ average size of 18.9 nm. Phase parameters were studied by powder X-ray diffraction (XRD), and the magnetic behavior of Fe(3)O(4) NPs was elucidated by Mössbauer spectroscopy. The colloidal and chemical stability of NPs was studied using simulated body fluid (phosphate buffer—PBS). Modified NPs have shown excellent stability in PBS (pH = 7.4), characterized by XRD, Mössbauer spectroscopy, and dynamic light scattering (DLS). Biocompatibility was evaluated in-vitro using cultured mouse embryonic fibroblasts (MEFs). The results show us an increasing of IC(50) from 0.110 mg/mL for Fe(3)O(4) NPs to 0.405 mg/mL for Fe(3)O(4)-Carborane NPs. The obtained data confirm the biocompatibility and stability of synthesized NPs and the potential to use them in BNCT. MDPI 2019-03-31 /pmc/articles/PMC6523109/ /pubmed/30935156 http://dx.doi.org/10.3390/nano9040494 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dukenbayev, Kanat Korolkov, Ilya V. Tishkevich, Daria I. Kozlovskiy, Artem L. Trukhanov, Sergey V. Gorin, Yevgeniy G. Shumskaya, Elena E. Kaniukov, Egor Y. Vinnik, Denis A. Zdorovets, Maxim V. Anisovich, Marina Trukhanov, Alex V. Tosi, Daniele Molardi, Carlo Fe(3)O(4) Nanoparticles for Complex Targeted Delivery and Boron Neutron Capture Therapy |
title | Fe(3)O(4) Nanoparticles for Complex Targeted Delivery and Boron Neutron Capture Therapy |
title_full | Fe(3)O(4) Nanoparticles for Complex Targeted Delivery and Boron Neutron Capture Therapy |
title_fullStr | Fe(3)O(4) Nanoparticles for Complex Targeted Delivery and Boron Neutron Capture Therapy |
title_full_unstemmed | Fe(3)O(4) Nanoparticles for Complex Targeted Delivery and Boron Neutron Capture Therapy |
title_short | Fe(3)O(4) Nanoparticles for Complex Targeted Delivery and Boron Neutron Capture Therapy |
title_sort | fe(3)o(4) nanoparticles for complex targeted delivery and boron neutron capture therapy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523109/ https://www.ncbi.nlm.nih.gov/pubmed/30935156 http://dx.doi.org/10.3390/nano9040494 |
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