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Bioelectrochemical Properties of Enzyme-Containing Multilayer Polyelectrolyte Microcapsules Modified with Multiwalled Carbon Nanotubes
This work investigated changes in the biochemical parameters of multilayer membrane structures, emerging at their modification with multiwalled carbon nanotubes (MWCNTs). The structures were represented by polyelectrolyte microcapsules (PMCs) containing glucose oxidase (GOx). PMCs were made using so...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523181/ https://www.ncbi.nlm.nih.gov/pubmed/31013718 http://dx.doi.org/10.3390/membranes9040053 |
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author | Reshetilov, Anatoly Plekhanova, Yulia Tarasov, Sergei Tikhonenko, Sergei Dubrovsky, Alexey Kim, Alexander Kashin, Vadim Machulin, Andrey Wang, Gou-Jen Kolesov, Vladimir Kuznetsova, Iren |
author_facet | Reshetilov, Anatoly Plekhanova, Yulia Tarasov, Sergei Tikhonenko, Sergei Dubrovsky, Alexey Kim, Alexander Kashin, Vadim Machulin, Andrey Wang, Gou-Jen Kolesov, Vladimir Kuznetsova, Iren |
author_sort | Reshetilov, Anatoly |
collection | PubMed |
description | This work investigated changes in the biochemical parameters of multilayer membrane structures, emerging at their modification with multiwalled carbon nanotubes (MWCNTs). The structures were represented by polyelectrolyte microcapsules (PMCs) containing glucose oxidase (GOx). PMCs were made using sodium polystyrene sulfonate (polyanion) and poly(allylamine hydrochloride) (polycation). Three compositions were considered: with MWCNTs incorporated between polyelectrolyte layers; with MWCNTs inserted into the hollow of the microcapsule; and with MWCNTs incorporated simultaneously into the hollow and between polyelectrolyte layers. The impedance spectra showed modifications using MWCNTs to cause a significant decrease in the PMC active resistance from 2560 to 25 kOhm. The cyclic current–voltage curves featured a current rise at modifications of multilayer MWCNT structures. A PMC-based composition was the basis of a receptor element of an amperometric biosensor. The sensitivity of glucose detection by the biosensor was 0.30 and 0.05 μA/mM for PMCs/MWCNTs/GOx and PMCs/GOx compositions, respectively. The biosensor was insensitive to the presence of ethanol or citric acid in the sample. Polyelectrolyte microcapsules based on a multilayer membrane incorporating the enzyme and MWCNTs can be efficient in developing biosensors and microbial fuel cells. |
format | Online Article Text |
id | pubmed-6523181 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65231812019-06-03 Bioelectrochemical Properties of Enzyme-Containing Multilayer Polyelectrolyte Microcapsules Modified with Multiwalled Carbon Nanotubes Reshetilov, Anatoly Plekhanova, Yulia Tarasov, Sergei Tikhonenko, Sergei Dubrovsky, Alexey Kim, Alexander Kashin, Vadim Machulin, Andrey Wang, Gou-Jen Kolesov, Vladimir Kuznetsova, Iren Membranes (Basel) Article This work investigated changes in the biochemical parameters of multilayer membrane structures, emerging at their modification with multiwalled carbon nanotubes (MWCNTs). The structures were represented by polyelectrolyte microcapsules (PMCs) containing glucose oxidase (GOx). PMCs were made using sodium polystyrene sulfonate (polyanion) and poly(allylamine hydrochloride) (polycation). Three compositions were considered: with MWCNTs incorporated between polyelectrolyte layers; with MWCNTs inserted into the hollow of the microcapsule; and with MWCNTs incorporated simultaneously into the hollow and between polyelectrolyte layers. The impedance spectra showed modifications using MWCNTs to cause a significant decrease in the PMC active resistance from 2560 to 25 kOhm. The cyclic current–voltage curves featured a current rise at modifications of multilayer MWCNT structures. A PMC-based composition was the basis of a receptor element of an amperometric biosensor. The sensitivity of glucose detection by the biosensor was 0.30 and 0.05 μA/mM for PMCs/MWCNTs/GOx and PMCs/GOx compositions, respectively. The biosensor was insensitive to the presence of ethanol or citric acid in the sample. Polyelectrolyte microcapsules based on a multilayer membrane incorporating the enzyme and MWCNTs can be efficient in developing biosensors and microbial fuel cells. MDPI 2019-04-12 /pmc/articles/PMC6523181/ /pubmed/31013718 http://dx.doi.org/10.3390/membranes9040053 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Reshetilov, Anatoly Plekhanova, Yulia Tarasov, Sergei Tikhonenko, Sergei Dubrovsky, Alexey Kim, Alexander Kashin, Vadim Machulin, Andrey Wang, Gou-Jen Kolesov, Vladimir Kuznetsova, Iren Bioelectrochemical Properties of Enzyme-Containing Multilayer Polyelectrolyte Microcapsules Modified with Multiwalled Carbon Nanotubes |
title | Bioelectrochemical Properties of Enzyme-Containing Multilayer Polyelectrolyte Microcapsules Modified with Multiwalled Carbon Nanotubes |
title_full | Bioelectrochemical Properties of Enzyme-Containing Multilayer Polyelectrolyte Microcapsules Modified with Multiwalled Carbon Nanotubes |
title_fullStr | Bioelectrochemical Properties of Enzyme-Containing Multilayer Polyelectrolyte Microcapsules Modified with Multiwalled Carbon Nanotubes |
title_full_unstemmed | Bioelectrochemical Properties of Enzyme-Containing Multilayer Polyelectrolyte Microcapsules Modified with Multiwalled Carbon Nanotubes |
title_short | Bioelectrochemical Properties of Enzyme-Containing Multilayer Polyelectrolyte Microcapsules Modified with Multiwalled Carbon Nanotubes |
title_sort | bioelectrochemical properties of enzyme-containing multilayer polyelectrolyte microcapsules modified with multiwalled carbon nanotubes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523181/ https://www.ncbi.nlm.nih.gov/pubmed/31013718 http://dx.doi.org/10.3390/membranes9040053 |
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