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The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles
The floral perianth, comprising sepals and petals, conceals the sexual organs and attracts pollinators. The coordination of growth and scent emission is not fully understood. We have analyzed the effect of knocking down CHANEL (PhCHL), the ZEITLUPE ortholog in petunia (PhCHL) by hairpin RNAs. Plants...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523265/ https://www.ncbi.nlm.nih.gov/pubmed/30979023 http://dx.doi.org/10.3390/cells8040343 |
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author | Terry, Marta I. Pérez-Sanz, Fernando Díaz-Galián, M. Victoria Pérez de los Cobos, Felipe Navarro, Pedro J. Egea-Cortines, Marcos Weiss, Julia |
author_facet | Terry, Marta I. Pérez-Sanz, Fernando Díaz-Galián, M. Victoria Pérez de los Cobos, Felipe Navarro, Pedro J. Egea-Cortines, Marcos Weiss, Julia |
author_sort | Terry, Marta I. |
collection | PubMed |
description | The floral perianth, comprising sepals and petals, conceals the sexual organs and attracts pollinators. The coordination of growth and scent emission is not fully understood. We have analyzed the effect of knocking down CHANEL (PhCHL), the ZEITLUPE ortholog in petunia (PhCHL) by hairpin RNAs. Plants with low PhCHL mRNA had overall decreased size. Growth evaluation using time lapse image analysis showed that early leaf movement was not affected by RNAi:PhCHL, but flower angle movement was modified, moving earlier during the day in knockdown plants than in wild types. Despite differences in stem length, growth rate was not significantly affected by loss of PhCHL. In contrast, petal growth displayed lower growth rate in RNAi:PhCHL. Decreased levels of PhCHL caused strongly modified scent profiles, including changes in composition and timing of emission resulting in volatile profiles highly divergent from the wild type. Our results show a role of PhCHL in controlling growth and development of vegetative and reproductive organs in petunia. The different effects of PhCHL on organ development indicate an organ-specific interpretation of the down regulation of PhCHL. Through the control of both timing and quantitative volatile emissions, PhCHL appears to be a major coordinator of scent profiles. |
format | Online Article Text |
id | pubmed-6523265 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65232652019-06-03 The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles Terry, Marta I. Pérez-Sanz, Fernando Díaz-Galián, M. Victoria Pérez de los Cobos, Felipe Navarro, Pedro J. Egea-Cortines, Marcos Weiss, Julia Cells Article The floral perianth, comprising sepals and petals, conceals the sexual organs and attracts pollinators. The coordination of growth and scent emission is not fully understood. We have analyzed the effect of knocking down CHANEL (PhCHL), the ZEITLUPE ortholog in petunia (PhCHL) by hairpin RNAs. Plants with low PhCHL mRNA had overall decreased size. Growth evaluation using time lapse image analysis showed that early leaf movement was not affected by RNAi:PhCHL, but flower angle movement was modified, moving earlier during the day in knockdown plants than in wild types. Despite differences in stem length, growth rate was not significantly affected by loss of PhCHL. In contrast, petal growth displayed lower growth rate in RNAi:PhCHL. Decreased levels of PhCHL caused strongly modified scent profiles, including changes in composition and timing of emission resulting in volatile profiles highly divergent from the wild type. Our results show a role of PhCHL in controlling growth and development of vegetative and reproductive organs in petunia. The different effects of PhCHL on organ development indicate an organ-specific interpretation of the down regulation of PhCHL. Through the control of both timing and quantitative volatile emissions, PhCHL appears to be a major coordinator of scent profiles. MDPI 2019-04-11 /pmc/articles/PMC6523265/ /pubmed/30979023 http://dx.doi.org/10.3390/cells8040343 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Terry, Marta I. Pérez-Sanz, Fernando Díaz-Galián, M. Victoria Pérez de los Cobos, Felipe Navarro, Pedro J. Egea-Cortines, Marcos Weiss, Julia The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles |
title | The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles |
title_full | The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles |
title_fullStr | The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles |
title_full_unstemmed | The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles |
title_short | The Petunia CHANEL Gene is a ZEITLUPE Ortholog Coordinating Growth and Scent Profiles |
title_sort | petunia chanel gene is a zeitlupe ortholog coordinating growth and scent profiles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523265/ https://www.ncbi.nlm.nih.gov/pubmed/30979023 http://dx.doi.org/10.3390/cells8040343 |
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