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miR-194 Accelerates Apoptosis of Aβ(1–42)-Transduced Hippocampal Neurons by Inhibiting Nrn1 and Decreasing PI3K/Akt Signaling Pathway Activity
This article explores the mechanism of miR-194 on the proliferation and apoptosis of Aβ(1–42)-transduced hippocampal neurons. Aβ(1–42)-transduced hippocampal neuron model was established by inducing hippocampal neurons with Aβ(1–42). MTT assay and flow cytometry were used to detect the viability and...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523401/ https://www.ncbi.nlm.nih.gov/pubmed/31010100 http://dx.doi.org/10.3390/genes10040313 |
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author | Wang, Tingting Cheng, Yaling Han, Haibin Liu, Jie Tian, Bo Liu, Xiaocui |
author_facet | Wang, Tingting Cheng, Yaling Han, Haibin Liu, Jie Tian, Bo Liu, Xiaocui |
author_sort | Wang, Tingting |
collection | PubMed |
description | This article explores the mechanism of miR-194 on the proliferation and apoptosis of Aβ(1–42)-transduced hippocampal neurons. Aβ(1–42)-transduced hippocampal neuron model was established by inducing hippocampal neurons with Aβ(1–42). MTT assay and flow cytometry were used to detect the viability and apoptosis of hippocampal neurons, respectively. qRT-PCR was used to detect changes in miR-194 and Nrn1 expression after Aβ(1–42) induction. Aβ(1–42)-transduced hippocampal neurons were transfected with miR-194 mimics and/or Nrn1 overexpression vectors. Their viability and neurite length were detected by MTT assay and immunofluorescence, respectively. Western blot was used to detect protein expression. Aβ(1–42) inhibited Aβ(1–42)-transduced hippocampal neuron activity and promoted their apoptosis in a dose-dependent manner. miR-194 was upregulated and Nrn1 was downregulated in Aβ(1–42)-transduced hippocampal neurons (p < 0.05). Compared with the model group, Aβ(1–42)-transduced hippocampal neurons of the miR-194 mimic group had much lower activity, average longest neurite length, Nrn1, p-AkT, and Bcl-2 protein expression and had much higher Bax, Caspase-3, and Cleaved Caspase-3 protein expression. Compared with the model group, Aβ(1–42)-transduced hippocampal neurons of the LV-Nrn1 group had much higher activity, average longest neurite length, Nrn1, p-AkT, and Bcl-2 protein expression and had much lower Bax, Caspase-3, and Cleaved Caspase-3 protein expression. Nrn1 is a target gene of miR-194. miR-194 inhibited apoptosis of Aβ(1–42)-transduced hippocampal neurons by inhibiting Nrn1 and decreasing PI3K/AkT signaling pathway activity. |
format | Online Article Text |
id | pubmed-6523401 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65234012019-06-03 miR-194 Accelerates Apoptosis of Aβ(1–42)-Transduced Hippocampal Neurons by Inhibiting Nrn1 and Decreasing PI3K/Akt Signaling Pathway Activity Wang, Tingting Cheng, Yaling Han, Haibin Liu, Jie Tian, Bo Liu, Xiaocui Genes (Basel) Article This article explores the mechanism of miR-194 on the proliferation and apoptosis of Aβ(1–42)-transduced hippocampal neurons. Aβ(1–42)-transduced hippocampal neuron model was established by inducing hippocampal neurons with Aβ(1–42). MTT assay and flow cytometry were used to detect the viability and apoptosis of hippocampal neurons, respectively. qRT-PCR was used to detect changes in miR-194 and Nrn1 expression after Aβ(1–42) induction. Aβ(1–42)-transduced hippocampal neurons were transfected with miR-194 mimics and/or Nrn1 overexpression vectors. Their viability and neurite length were detected by MTT assay and immunofluorescence, respectively. Western blot was used to detect protein expression. Aβ(1–42) inhibited Aβ(1–42)-transduced hippocampal neuron activity and promoted their apoptosis in a dose-dependent manner. miR-194 was upregulated and Nrn1 was downregulated in Aβ(1–42)-transduced hippocampal neurons (p < 0.05). Compared with the model group, Aβ(1–42)-transduced hippocampal neurons of the miR-194 mimic group had much lower activity, average longest neurite length, Nrn1, p-AkT, and Bcl-2 protein expression and had much higher Bax, Caspase-3, and Cleaved Caspase-3 protein expression. Compared with the model group, Aβ(1–42)-transduced hippocampal neurons of the LV-Nrn1 group had much higher activity, average longest neurite length, Nrn1, p-AkT, and Bcl-2 protein expression and had much lower Bax, Caspase-3, and Cleaved Caspase-3 protein expression. Nrn1 is a target gene of miR-194. miR-194 inhibited apoptosis of Aβ(1–42)-transduced hippocampal neurons by inhibiting Nrn1 and decreasing PI3K/AkT signaling pathway activity. MDPI 2019-04-21 /pmc/articles/PMC6523401/ /pubmed/31010100 http://dx.doi.org/10.3390/genes10040313 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Tingting Cheng, Yaling Han, Haibin Liu, Jie Tian, Bo Liu, Xiaocui miR-194 Accelerates Apoptosis of Aβ(1–42)-Transduced Hippocampal Neurons by Inhibiting Nrn1 and Decreasing PI3K/Akt Signaling Pathway Activity |
title | miR-194 Accelerates Apoptosis of Aβ(1–42)-Transduced Hippocampal Neurons by Inhibiting Nrn1 and Decreasing PI3K/Akt Signaling Pathway Activity |
title_full | miR-194 Accelerates Apoptosis of Aβ(1–42)-Transduced Hippocampal Neurons by Inhibiting Nrn1 and Decreasing PI3K/Akt Signaling Pathway Activity |
title_fullStr | miR-194 Accelerates Apoptosis of Aβ(1–42)-Transduced Hippocampal Neurons by Inhibiting Nrn1 and Decreasing PI3K/Akt Signaling Pathway Activity |
title_full_unstemmed | miR-194 Accelerates Apoptosis of Aβ(1–42)-Transduced Hippocampal Neurons by Inhibiting Nrn1 and Decreasing PI3K/Akt Signaling Pathway Activity |
title_short | miR-194 Accelerates Apoptosis of Aβ(1–42)-Transduced Hippocampal Neurons by Inhibiting Nrn1 and Decreasing PI3K/Akt Signaling Pathway Activity |
title_sort | mir-194 accelerates apoptosis of aβ(1–42)-transduced hippocampal neurons by inhibiting nrn1 and decreasing pi3k/akt signaling pathway activity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523401/ https://www.ncbi.nlm.nih.gov/pubmed/31010100 http://dx.doi.org/10.3390/genes10040313 |
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