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Role of Intestinal Microbiota in Metabolism of Gastrodin In Vitro and In Vivo

Alteration in the number and composition of intestinal microbiota affects the metabolism of several xenobiotics. Gastrodin, isolated from Gastrodia elata, is prone to be hydrolyzed by intestinal microbiota. In the present study, the role of intestinal microbiota in gastrodin metabolism was investiga...

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Autores principales: Nepal, Mahesh Raj, Jeong, Ki Sun, Kim, Geon Ho, Cha, Dong Ho, Kang, Mi Jeong, Kim, Jin Sung, Kim, Ju-Hyun, Jeong, Tae Cheon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523420/
https://www.ncbi.nlm.nih.gov/pubmed/30965644
http://dx.doi.org/10.3390/metabo9040069
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author Nepal, Mahesh Raj
Jeong, Ki Sun
Kim, Geon Ho
Cha, Dong Ho
Kang, Mi Jeong
Kim, Jin Sung
Kim, Ju-Hyun
Jeong, Tae Cheon
author_facet Nepal, Mahesh Raj
Jeong, Ki Sun
Kim, Geon Ho
Cha, Dong Ho
Kang, Mi Jeong
Kim, Jin Sung
Kim, Ju-Hyun
Jeong, Tae Cheon
author_sort Nepal, Mahesh Raj
collection PubMed
description Alteration in the number and composition of intestinal microbiota affects the metabolism of several xenobiotics. Gastrodin, isolated from Gastrodia elata, is prone to be hydrolyzed by intestinal microbiota. In the present study, the role of intestinal microbiota in gastrodin metabolism was investigated in vitro and in vivo. Gastrodin was incubated in an anaerobic condition with intestinal contents prepared from vehicle- and antibiotics-treated rats and the disappearance of gastrodin and formation of 4-hydroxybenzyl alcohol (4-HBA) was measured by liquid chromatography coupled to mass spectroscopy (LC-MS/MS). The results showed that almost all gastrodin incubated with control intestinal contents was metabolized to its aglycone in time- and concentration-dependent manners. In contrast, much less formation of 4-HBA was detected in intestinal contents from antibiotics-treated rats. Subsequently, in vivo pharmacokinetic study revealed that the antibiotic pretreatment of rats significantly affected the metabolism of gastrodin to 4-HBA. When administered orally, gastrodin was rapidly absorbed rapidly into plasma, metabolized to 4-HBA, and disappeared from the body within six hours. Interestingly, the pharmacokinetic parameters of 4-HBA were changed remarkably in antibiotics-treated rats, compared to control rats. The results clearly indicated that the antibiotics treatment of rats suppressed the ability of intestinal microbiota to metabolize gastrodin to 4-HBA and that, thereby, the pharmacodynamic action was significantly modulated.
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spelling pubmed-65234202019-06-03 Role of Intestinal Microbiota in Metabolism of Gastrodin In Vitro and In Vivo Nepal, Mahesh Raj Jeong, Ki Sun Kim, Geon Ho Cha, Dong Ho Kang, Mi Jeong Kim, Jin Sung Kim, Ju-Hyun Jeong, Tae Cheon Metabolites Article Alteration in the number and composition of intestinal microbiota affects the metabolism of several xenobiotics. Gastrodin, isolated from Gastrodia elata, is prone to be hydrolyzed by intestinal microbiota. In the present study, the role of intestinal microbiota in gastrodin metabolism was investigated in vitro and in vivo. Gastrodin was incubated in an anaerobic condition with intestinal contents prepared from vehicle- and antibiotics-treated rats and the disappearance of gastrodin and formation of 4-hydroxybenzyl alcohol (4-HBA) was measured by liquid chromatography coupled to mass spectroscopy (LC-MS/MS). The results showed that almost all gastrodin incubated with control intestinal contents was metabolized to its aglycone in time- and concentration-dependent manners. In contrast, much less formation of 4-HBA was detected in intestinal contents from antibiotics-treated rats. Subsequently, in vivo pharmacokinetic study revealed that the antibiotic pretreatment of rats significantly affected the metabolism of gastrodin to 4-HBA. When administered orally, gastrodin was rapidly absorbed rapidly into plasma, metabolized to 4-HBA, and disappeared from the body within six hours. Interestingly, the pharmacokinetic parameters of 4-HBA were changed remarkably in antibiotics-treated rats, compared to control rats. The results clearly indicated that the antibiotics treatment of rats suppressed the ability of intestinal microbiota to metabolize gastrodin to 4-HBA and that, thereby, the pharmacodynamic action was significantly modulated. MDPI 2019-04-08 /pmc/articles/PMC6523420/ /pubmed/30965644 http://dx.doi.org/10.3390/metabo9040069 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nepal, Mahesh Raj
Jeong, Ki Sun
Kim, Geon Ho
Cha, Dong Ho
Kang, Mi Jeong
Kim, Jin Sung
Kim, Ju-Hyun
Jeong, Tae Cheon
Role of Intestinal Microbiota in Metabolism of Gastrodin In Vitro and In Vivo
title Role of Intestinal Microbiota in Metabolism of Gastrodin In Vitro and In Vivo
title_full Role of Intestinal Microbiota in Metabolism of Gastrodin In Vitro and In Vivo
title_fullStr Role of Intestinal Microbiota in Metabolism of Gastrodin In Vitro and In Vivo
title_full_unstemmed Role of Intestinal Microbiota in Metabolism of Gastrodin In Vitro and In Vivo
title_short Role of Intestinal Microbiota in Metabolism of Gastrodin In Vitro and In Vivo
title_sort role of intestinal microbiota in metabolism of gastrodin in vitro and in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523420/
https://www.ncbi.nlm.nih.gov/pubmed/30965644
http://dx.doi.org/10.3390/metabo9040069
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