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Role of Intestinal Microbiota in Metabolism of Gastrodin In Vitro and In Vivo
Alteration in the number and composition of intestinal microbiota affects the metabolism of several xenobiotics. Gastrodin, isolated from Gastrodia elata, is prone to be hydrolyzed by intestinal microbiota. In the present study, the role of intestinal microbiota in gastrodin metabolism was investiga...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523420/ https://www.ncbi.nlm.nih.gov/pubmed/30965644 http://dx.doi.org/10.3390/metabo9040069 |
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author | Nepal, Mahesh Raj Jeong, Ki Sun Kim, Geon Ho Cha, Dong Ho Kang, Mi Jeong Kim, Jin Sung Kim, Ju-Hyun Jeong, Tae Cheon |
author_facet | Nepal, Mahesh Raj Jeong, Ki Sun Kim, Geon Ho Cha, Dong Ho Kang, Mi Jeong Kim, Jin Sung Kim, Ju-Hyun Jeong, Tae Cheon |
author_sort | Nepal, Mahesh Raj |
collection | PubMed |
description | Alteration in the number and composition of intestinal microbiota affects the metabolism of several xenobiotics. Gastrodin, isolated from Gastrodia elata, is prone to be hydrolyzed by intestinal microbiota. In the present study, the role of intestinal microbiota in gastrodin metabolism was investigated in vitro and in vivo. Gastrodin was incubated in an anaerobic condition with intestinal contents prepared from vehicle- and antibiotics-treated rats and the disappearance of gastrodin and formation of 4-hydroxybenzyl alcohol (4-HBA) was measured by liquid chromatography coupled to mass spectroscopy (LC-MS/MS). The results showed that almost all gastrodin incubated with control intestinal contents was metabolized to its aglycone in time- and concentration-dependent manners. In contrast, much less formation of 4-HBA was detected in intestinal contents from antibiotics-treated rats. Subsequently, in vivo pharmacokinetic study revealed that the antibiotic pretreatment of rats significantly affected the metabolism of gastrodin to 4-HBA. When administered orally, gastrodin was rapidly absorbed rapidly into plasma, metabolized to 4-HBA, and disappeared from the body within six hours. Interestingly, the pharmacokinetic parameters of 4-HBA were changed remarkably in antibiotics-treated rats, compared to control rats. The results clearly indicated that the antibiotics treatment of rats suppressed the ability of intestinal microbiota to metabolize gastrodin to 4-HBA and that, thereby, the pharmacodynamic action was significantly modulated. |
format | Online Article Text |
id | pubmed-6523420 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65234202019-06-03 Role of Intestinal Microbiota in Metabolism of Gastrodin In Vitro and In Vivo Nepal, Mahesh Raj Jeong, Ki Sun Kim, Geon Ho Cha, Dong Ho Kang, Mi Jeong Kim, Jin Sung Kim, Ju-Hyun Jeong, Tae Cheon Metabolites Article Alteration in the number and composition of intestinal microbiota affects the metabolism of several xenobiotics. Gastrodin, isolated from Gastrodia elata, is prone to be hydrolyzed by intestinal microbiota. In the present study, the role of intestinal microbiota in gastrodin metabolism was investigated in vitro and in vivo. Gastrodin was incubated in an anaerobic condition with intestinal contents prepared from vehicle- and antibiotics-treated rats and the disappearance of gastrodin and formation of 4-hydroxybenzyl alcohol (4-HBA) was measured by liquid chromatography coupled to mass spectroscopy (LC-MS/MS). The results showed that almost all gastrodin incubated with control intestinal contents was metabolized to its aglycone in time- and concentration-dependent manners. In contrast, much less formation of 4-HBA was detected in intestinal contents from antibiotics-treated rats. Subsequently, in vivo pharmacokinetic study revealed that the antibiotic pretreatment of rats significantly affected the metabolism of gastrodin to 4-HBA. When administered orally, gastrodin was rapidly absorbed rapidly into plasma, metabolized to 4-HBA, and disappeared from the body within six hours. Interestingly, the pharmacokinetic parameters of 4-HBA were changed remarkably in antibiotics-treated rats, compared to control rats. The results clearly indicated that the antibiotics treatment of rats suppressed the ability of intestinal microbiota to metabolize gastrodin to 4-HBA and that, thereby, the pharmacodynamic action was significantly modulated. MDPI 2019-04-08 /pmc/articles/PMC6523420/ /pubmed/30965644 http://dx.doi.org/10.3390/metabo9040069 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nepal, Mahesh Raj Jeong, Ki Sun Kim, Geon Ho Cha, Dong Ho Kang, Mi Jeong Kim, Jin Sung Kim, Ju-Hyun Jeong, Tae Cheon Role of Intestinal Microbiota in Metabolism of Gastrodin In Vitro and In Vivo |
title | Role of Intestinal Microbiota in Metabolism of Gastrodin In Vitro and In Vivo |
title_full | Role of Intestinal Microbiota in Metabolism of Gastrodin In Vitro and In Vivo |
title_fullStr | Role of Intestinal Microbiota in Metabolism of Gastrodin In Vitro and In Vivo |
title_full_unstemmed | Role of Intestinal Microbiota in Metabolism of Gastrodin In Vitro and In Vivo |
title_short | Role of Intestinal Microbiota in Metabolism of Gastrodin In Vitro and In Vivo |
title_sort | role of intestinal microbiota in metabolism of gastrodin in vitro and in vivo |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523420/ https://www.ncbi.nlm.nih.gov/pubmed/30965644 http://dx.doi.org/10.3390/metabo9040069 |
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